| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Remi Cavagna | Soudal Quick-Step | 04:03:03 | |
| 2 | Mauro Schmid | Soudal Quick-Step | + 32 | + 32 |
| 3 | Rick Pluimers | Tudor Pro Cycling Team | + 32 | + 32 |
| 4 | Loe van Belle | Jumbo-Visma | + 32 | + 32 |
| 5 | Natnael Tesfazion | Lidl-Trek | + 32 | + 32 |
| 6 | Cristian Scaroni | Astana Qazaqstan Team | + 32 | + 32 |
| 7 | Davide Gabburo | Green Project-Bardiani CSF-Faizanè | + 32 | + 32 |
| 8 | Walter Calzoni | Q36.5 Pro Cycling Team | + 32 | + 32 |
| 9 | Milan Vader | Jumbo-Visma | + 32 | + 32 |
| 10 | Luc Wirtgen | Tudor Pro Cycling Team | + 32 | + 32 |
| 11 | Thomas Pesenti | Beltrami TSA-Tre Colli | + 32 | + 32 |
| 12 | Felix Engelhardt | Team Jayco-AlUla | + 32 | + 32 |
| 13 | Tijmen Graat | Jumbo-Visma | + 32 | + 32 |
| 14 | Marco Tizza | Bingoal WB | + 32 | + 32 |
| 15 | Ben Zwiehoff | BORA-hansgrohe | + 32 | + 32 |
| 16 | Alessio Martinelli | Green Project-Bardiani CSF-Faizanè | + 32 | + 32 |
| 17 | James Knox | Soudal Quick-Step | + 32 | + 32 |
| 18 | Sean Quinn | EF Education-EasyPost | + 32 | + 32 |
| 19 | Junior Lecerf William | Soudal Quick-Step | + 32 | + 32 |
| 20 | Alex Martin | EOLO-Kometa Cycling Team | + 32 | + 32 |
| 21 | Leo Hayter | INEOS Grenadiers | + 32 | + 32 |
| 22 | Luca Covili | Green Project-Bardiani CSF-Faizanè | + 32 | + 32 |
| 23 | Ben Healy | EF Education-EasyPost | + 32 | + 32 |
| 24 | Domenico Pozzovivo | Israel-Premier Tech | + 32 | + 32 |
| 25 | James Shaw | EF Education-EasyPost | + 32 | + 32 |
| 26 | Fernando Tercero | EOLO-Kometa Cycling Team | + 32 | + 32 |
| 27 | Alessandro Monaco | Team Technipes #InEmiliaRomagna | + 32 | + 32 |
| 28 | Gijs Leemreize | Jumbo-Visma | + 32 | + 32 |
| 29 | Stefan de Bod | EF Education-EasyPost | + 32 | + 32 |
| 30 | Aloïs Charrin | Tudor Pro Cycling Team | + 32 | + 32 |
| 31 | Marco Frigo | Israel-Premier Tech | + 32 | + 32 |
| 32 | Mark Padun | EF Education-EasyPost | + 32 | + 32 |
| 33 | Mathias Vacek | Lidl-Trek | + 32 | + 32 |
| 34 | Floris de Tier | Bingoal WB | + 32 | + 32 |
| 35 | Tony Gallopin | Lidl-Trek | + 32 | + 32 |
| 36 | Gianluca Brambilla | Q36.5 Pro Cycling Team | + 32 | + 32 |
| 37 | Johannes Staune-mittet | Jumbo-Visma | + 32 | + 32 |
| 38 | Valerio Conti | Corratec Selle Italia | + 01:54 | + 01:54 |
| 39 | Luis-joe Lührs | BORA-hansgrohe | + 01:54 | + 01:54 |
| 40 | Antonio Nibali | Astana Qazaqstan Team | + 01:54 | + 01:54 |
| 41 | Alessandro Pinarello | Green Project-Bardiani CSF-Faizanè | + 01:54 | + 01:54 |
| 42 | Andrés Mancipe | GW Shimano Sidermec | + 01:54 | + 01:54 |
| 43 | Alessandro Bisolti | GW Shimano Sidermec | + 01:54 | + 01:54 |
| 44 | Omar El Gouzi | Green Project-Bardiani CSF-Faizanè | + 01:54 | + 01:54 |
| 45 | Martin Nessler | Team Technipes #InEmiliaRomagna | + 01:54 | + 01:54 |
| 46 | Ben Hermans | Israel-Premier Tech | + 01:54 | + 01:54 |
| 47 | Mark Stewart | Bolton Equities Black Spoke | + 01:54 | + 01:54 |
| 48 | Davide Piganzoli | EOLO-Kometa Cycling Team | + 01:54 | + 01:54 |
| 49 | Brandon Rivera | INEOS Grenadiers | + 01:54 | + 01:54 |
| 50 | Francesco Gavazzi | EOLO-Kometa Cycling Team | + 01:54 | + 01:54 |
| 51 | Amanuel Ghebreigzabhier | Lidl-Trek | + 01:54 | + 01:54 |
| 52 | James Oram | Bolton Equities Black Spoke | + 01:54 | + 01:54 |
| 53 | Lawson Craddock | Team Jayco-AlUla | + 01:54 | + 01:54 |
| 54 | Thomas Gloag | Jumbo-Visma | + 03:57 | + 03:57 |
| 55 | Rudy Porter | Team Jayco-AlUla | + 03:57 | + 03:57 |
| 56 | Dimitri Peyskens | Bingoal WB | + 03:57 | + 03:57 |
| 57 | Patrick Gamper | BORA-hansgrohe | + 03:57 | + 03:57 |
| 58 | Florian Lipowitz | BORA-hansgrohe | + 03:57 | + 03:57 |
| 59 | Giacomo Garavaglia | Work Service-Vitalcare-Dynatek | + 03:57 | + 03:57 |
| 60 | Martin Svrcek | Soudal Quick-Step | + 03:57 | + 03:57 |
| 61 | Fredrik Hagen Carl | Q36.5 Pro Cycling Team | + 03:57 | + 03:57 |
| 62 | Stan van Tricht | Soudal Quick-Step | + 03:57 | + 03:57 |
| 63 | Marcel Camprubí | Q36.5 Pro Cycling Team | + 03:57 | + 03:57 |
| 64 | Lennert Teugels | Bingoal WB | + 03:57 | + 03:57 |
| 65 | Jhonatan Restrepo | GW Shimano Sidermec | + 03:57 | + 03:57 |
| 66 | Elia Viviani | INEOS Grenadiers | + 05:44 | + 05:44 |
| 67 | Andrea Garosio | EOLO-Kometa Cycling Team | + 05:44 | + 05:44 |
| 68 | Georg Steinhauser | EF Education-EasyPost | + 05:44 | + 05:44 |
| 69 | Nadav Raisberg | Israel-Premier Tech | + 05:44 | + 05:44 |
| 70 | Manuele Boaro | Astana Qazaqstan Team | + 07:00 | + 07:00 |
| 71 | Hannes Wilksch | Tudor Pro Cycling Team | + 07:00 | + 07:00 |
| 72 | Alastair Mackellar | Israel-Premier Tech | + 07:00 | + 07:00 |
| 73 | Alexis Guérin | Bingoal WB | + 07:00 | + 07:00 |
| 74 | Gabriele Petrelli | Team Technipes #InEmiliaRomagna | + 08:02 | + 08:02 |
| 75 | Joel Suter | Tudor Pro Cycling Team | + 08:04 | + 08:04 |
| 76 | Fabian Weiss | Tudor Pro Cycling Team | + 11:45 | + 11:45 |
| 77 | Marco Murgano | Corratec Selle Italia | + 11:45 | + 11:45 |
| 78 | Veljko Stojnic | Corratec Selle Italia | + 15:17 | + 15:17 |
| 79 | Etienne van Empel | Corratec Selle Italia | + 15:17 | + 15:17 |
| 80 | Aaron van der Beken | Bingoal WB | + 15:17 | + 15:17 |
| 81 | Conor Schunk | Sofer-Savini Due-OMZ | + 15:17 | + 15:17 |
| 82 | Filippo D'aiuto | General Store-Essegibi-F.Ili Curia | + 15:17 | + 15:17 |
| 83 | Alex Raimondi | Beltrami TSA-Tre Colli | + 15:17 | + 15:17 |
| 84 | Matteo Freddi | Beltrami TSA-Tre Colli | + 15:17 | + 15:17 |
| 85 | Ben Granger | MG.K vis Colors for Peace VPM | + 15:17 | + 15:17 |
| 86 | Yuriy Natarov | Astana Qazaqstan Team | + 15:17 | + 15:17 |
| 87 | Roland Thalmann | Tudor Pro Cycling Team | + 15:17 | + 15:17 |
| 88 | Igor Chzhan | Astana Qazaqstan Team | + 15:17 | + 15:17 |
| 89 | Cameron Wurf | INEOS Grenadiers | + 15:17 | + 15:17 |
| 90 | Davide Dapporto | Team Technipes #InEmiliaRomagna | + 15:17 | + 15:17 |
| 91 | Samuele Carpene | General Store-Essegibi-F.Ili Curia | + 15:17 | + 15:17 |
| 92 | Michael Leonard | INEOS Grenadiers | + 15:17 | + 15:17 |
| 93 | Miguel Florez | GW Shimano Sidermec | + 15:17 | + 15:17 |
| 94 | Cyrus Monk | Q36.5 Pro Cycling Team | + 15:17 | + 15:17 |
| 95 | Marc Pritzen | EF Education-EasyPost | + 15:17 | + 15:17 |
| 96 | Emanuele Ansaloni | Team Technipes #InEmiliaRomagna | + 15:17 | + 15:17 |
| 97 | Stefano Gandin | Corratec Selle Italia | + 15:17 | + 15:17 |
| 98 | Tommaso Bergagna | General Store-Essegibi-F.Ili Curia | + 15:17 | + 15:17 |
| 99 | David Peña Jesus | Team Jayco-AlUla | + 15:17 | + 15:17 |
| 100 | Didier Merchan | GW Shimano Sidermec | + 15:17 | + 15:17 |
| 101 | Jhonatan Guatibonza | GW Shimano Sidermec | + 15:17 | + 15:17 |
| 102 | Diego Pescador | GW Shimano Sidermec | + 15:17 | + 15:17 |
| 103 | Alessandro Santaromita | Green Project-Bardiani CSF-Faizanè | + 15:17 | + 15:17 |
| 104 | Henok Mulubrhan | Green Project-Bardiani CSF-Faizanè | + 15:17 | + 15:17 |
| 105 | Alessandro Fancellu | EOLO-Kometa Cycling Team | + 15:17 | + 15:17 |
| 106 | Antonio Puppio | Q36.5 Pro Cycling Team | + 15:17 | + 15:17 |
| 107 | Kevin Bonaldo | Work Service-Vitalcare-Dynatek | + 15:17 | + 15:17 |
| 108 | Danilo Pase Christian | Work Service-Vitalcare-Dynatek | + 15:17 | + 15:17 |
| 109 | Corey Davis | Q36.5 Pro Cycling Team | + 15:17 | + 15:17 |
| 110 | Karel Vacek | Corratec Selle Italia | + 15:17 | + 15:17 |
| 111 | Remy Mertz | Bingoal WB | + 15:17 | + 15:17 |
| 112 | Michael Hepburn | Team Jayco-AlUla | + 15:17 | + 15:17 |
| 113 | Luke Rowe | INEOS Grenadiers | + 15:17 | + 15:17 |
| 114 | Josh Kench | Bolton Equities Black Spoke | + 15:17 | + 15:17 |
| 115 | James Fouche | Bolton Equities Black Spoke | + 15:17 | + 15:17 |
| 116 | Francesco Carollo | MG.K vis Colors for Peace VPM | + 15:17 | + 15:17 |
| 117 | Andrea Piras | Beltrami TSA-Tre Colli | + 15:17 | + 15:17 |
| 118 | Omer Goldstein | Israel-Premier Tech | + 15:17 | + 15:17 |
| 119 | Matthew Walls | BORA-hansgrohe | + 17:39 | + 17:39 |
| 120 | Michael Vanni | Beltrami TSA-Tre Colli | + 17:39 | + 17:39 |
| 121 | Davide Bauce | MG.K vis Colors for Peace VPM | + 17:39 | + 17:39 |
| 122 | Alexandr Riabushenko | Astana Qazaqstan Team | + 17:39 | + 17:39 |
| 123 | Gidas Umbri | Team Technipes #InEmiliaRomagna | + 17:39 | + 17:39 |
| 124 | Gleb Brussenskiy | Astana Qazaqstan Team | + 17:39 | + 17:39 |
| 125 | Lucio Pierantozzi | Beltrami TSA-Tre Colli | + 17:39 | + 17:39 |
| 126 | Simone Raccani | EOLO-Kometa Cycling Team | + 17:39 | + 17:39 |
| 127 | Josef Cerný | Soudal Quick-Step | + 17:39 | + 17:39 |
| 128 | Michele Berasi | General Store-Essegibi-F.Ili Curia | + 19:39 | + 19:39 |
| 129 | Lorenzo Peschi | General Store-Essegibi-F.Ili Curia | + 19:39 | + 19:39 |
| 130 | Nikiforos Arvanitou | Sofer-Savini Due-OMZ | + 19:39 | + 19:39 |
| 131 | Lapo Gavilli | MG.K vis Colors for Peace VPM | + 19:39 | + 19:39 |
| 132 | Antal Lepold Zoltán | Sofer-Savini Due-OMZ | + 19:39 | + 19:39 |
| 133 | Charlie Quarterman | Corratec Selle Italia | + 19:39 | + 19:39 |
| 134 | Fabio Mazzucco | MG.K vis Colors for Peace VPM | + 19:39 | + 19:39 |
| 135 | Stefano Leali | General Store-Essegibi-F.Ili Curia | + 19:39 | + 19:39 |
| 136 | Andrea Mearelli | MG.K vis Colors for Peace VPM | + 19:39 | + 19:39 |
| 137 | Nicola Venchiarutti | Work Service-Vitalcare-Dynatek | + 19:39 | + 19:39 |
| 138 | Bailey O'donnell | Bolton Equities Black Spoke | + 19:39 | + 19:39 |
| 139 | Kevin Pezzo Rosola | General Store-Essegibi-F.Ili Curia | + 19:39 | + 19:39 |
| 140 | Samuele Mion | Work Service-Vitalcare-Dynatek | + 19:39 | + 19:39 |
| 141 | Luca Tornaboni | Work Service-Vitalcare-Dynatek | + 19:39 | + 19:39 |
| 142 | Emil Dima | Sofer-Savini Due-OMZ | + 19:39 | + 19:39 |
| 143 | Logan Currie | Bolton Equities Black Spoke | + 19:39 | + 19:39 |
| 144 | Soh Narumi | Sofer-Savini Due-OMZ | + 19:39 | + 19:39 |
| 145 | Romaric Forques | Team Technipes #InEmiliaRomagna | + 19:39 | + 19:39 |
| 146 | Lorenzo Di Camillo | Sofer-Savini Due-OMZ | + 19:39 | + 19:39 |
| 147 | Andrea Biancalani | Beltrami TSA-Tre Colli | + 19:39 | + 19:39 |
| 148 | Hager Andemariam | Sofer-Savini Due-OMZ | + 19:39 | + 19:39 |