| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Daniel Martin | Garmin-Transitions | 04:51:20 | |
| 2 | Domenico Pozzovivo | Colnago - CSF Inox | + 06 | + 06 |
| 3 | Jérôme Baugnies | Topsport Vlaanderen - Mercator | + 12 | + 12 |
| 4 | Kristof Vandewalle | Topsport Vlaanderen - Mercator | + 12 | + 12 |
| 5 | Damiano Cunego | Lampre-Farnese Vini | + 12 | + 12 |
| 6 | Brian Vandborg | Liquigas-Doimo | + 12 | + 12 |
| 7 | Giovanni Visconti | ISD - Neri | + 12 | + 12 |
| 8 | Mauro Santambrogio | BMC Racing Team | + 12 | + 12 |
| 9 | Francesco Gavazzi | Lampre-Farnese Vini | + 12 | + 12 |
| 10 | Mauro Finetto | Liquigas-Doimo | + 12 | + 12 |
| 11 | Manuele Mori | Lampre-Farnese Vini | + 12 | + 12 |
| 12 | Leonardo Bertagnolli | Androni Giocattoli | + 12 | + 12 |
| 13 | Paolo Bailetti | Ceramica Flaminia | + 12 | + 12 |
| 14 | Marco Pinotti | Team HTC - Columbia | + 12 | + 12 |
| 15 | Jure Golcer | De Rosa - Stac Plastic | + 12 | + 12 |
| 16 | Vincenzo Nibali | Liquigas-Doimo | + 12 | + 12 |
| 17 | Gustavo Dominguez Lemos | Xacobeo-Galicia | + 50 | + 50 |
| 18 | Antonio de Segovia Jose | Xacobeo-Galicia | + 01:22 | + 01:22 |
| 19 | Jackson Rodriguez | Androni Giocattoli | + 01:22 | + 01:22 |
| 20 | Cristiano Salerno | De Rosa - Stac Plastic | + 01:22 | + 01:22 |
| 21 | Pieter Jacobs | Topsport Vlaanderen - Mercator | + 01:22 | + 01:22 |
| 22 | Przemyslaw Niemiec | Miche Pro Cycling Team | + 01:22 | + 01:22 |
| 23 | Cristiano Fumagalli | Ceramica Flaminia | + 01:22 | + 01:22 |
| 24 | Pieter Serry | Topsport Vlaanderen - Mercator | + 01:22 | + 01:22 |
| 25 | Daniele Colli | Ceramica Flaminia | + 01:22 | + 01:22 |
| 26 | Manuel Calvente | Andalucía - Cajasur | + 01:22 | + 01:22 |
| 27 | Eros Capecchi | Footon-Servetto | + 01:22 | + 01:22 |
| 28 | Michele Scarponi | Androni Giocattoli | + 01:22 | + 01:22 |
| 29 | Andrea Tonti | Carmiooro NGC | + 01:22 | + 01:22 |
| 30 | Sander Armee | Topsport Vlaanderen - Mercator | + 01:22 | + 01:22 |
| 31 | Fortunato Baliani | Miche Pro Cycling Team | + 01:22 | + 01:22 |
| 32 | Maxime Monfort | Team HTC - Columbia | + 01:22 | + 01:22 |
| 33 | Fredrik Kessiakoff | Garmin-Transitions | + 01:22 | + 01:22 |
| 34 | Massimiliano Gentili | Ceramica Flaminia | + 01:22 | + 01:22 |
| 35 | Martin Velits | Team HTC - Columbia | + 01:22 | + 01:22 |
| 36 | Francesco Failli | Acqua e Sapone | + 01:22 | + 01:22 |
| 37 | Christian Meier | Garmin-Transitions | + 01:22 | + 01:22 |
| 38 | José Gomez | Andalucía - Cajasur | + 01:22 | + 01:22 |
| 39 | Serafín Martinez | Xacobeo-Galicia | + 01:22 | + 01:22 |
| 40 | Karsten Kroon | BMC Racing Team | + 01:22 | + 01:22 |
| 41 | Diego Ulissi | Lampre-Farnese Vini | + 01:22 | + 01:22 |
| 42 | Federico Canuti | Colnago - CSF Inox | + 01:22 | + 01:22 |
| 43 | Valerio Agnoli | Liquigas-Doimo | + 01:22 | + 01:22 |
| 44 | Ivan Basso | Liquigas-Doimo | + 01:22 | + 01:22 |
| 45 | Marco Marzano | Lampre-Farnese Vini | + 01:22 | + 01:22 |
| 46 | Francesco Ginanni | Androni Giocattoli | + 01:22 | + 01:22 |
| 47 | Fabio Taborre | Androni Giocattoli | + 01:22 | + 01:22 |
| 48 | Davide Torosantucci | CDC - Cavaliere | + 01:22 | + 01:22 |
| 49 | Preben van Hecke | Topsport Vlaanderen - Mercator | + 01:22 | + 01:22 |
| 50 | Giairo Ermeti | De Rosa - Stac Plastic | + 01:22 | + 01:22 |
| 51 | Vladimir Miholjevic | Acqua e Sapone | + 01:22 | + 01:22 |
| 52 | Alessandro Ballan | BMC Racing Team | + 01:22 | + 01:22 |
| 53 | Daniele Callegarin | CDC - Cavaliere | + 01:22 | + 01:22 |
| 54 | Massimo Codol | Acqua e Sapone | + 01:22 | + 01:22 |
| 55 | Cayetano Sarmiento Jose | Acqua e Sapone | + 01:22 | + 01:22 |
| 56 | Davide Bonuccelli | CDC - Cavaliere | + 01:46 | + 01:46 |
| 57 | Antonio Piedra | Andalucía - Cajasur | + 01:46 | + 01:46 |
| 58 | Riccardo Chiarini | De Rosa - Stac Plastic | + 01:46 | + 01:46 |
| 59 | Daniel Diaz | Footon-Servetto | + 02:40 | + 02:40 |
| 60 | Tyler Farrar | Garmin-Transitions | + 03:05 | + 03:05 |
| 61 | Giuseppe Muraglia | CDC - Cavaliere | + 03:05 | + 03:05 |
| 62 | Geert Steurs | Topsport Vlaanderen - Mercator | + 03:30 | + 03:30 |