| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Manuel Belletti | Colnago - CSF Inox | 04:43:35 | |
| 2 | Danilo Hondo | Lampre-Farnese Vini | + 00 | |
| 3 | Mark Cavendish | Team HTC - Columbia | + 00 | |
| 4 | Daniele Colli | Ceramica Flaminia | + 00 | |
| 5 | Daniele Bennati | Liquigas-Doimo | + 00 | |
| 6 | Tyler Farrar | Garmin-Transitions | + 00 | |
| 7 | Daniele Ratto | Carmiooro NGC | + 00 | |
| 8 | Pierpaolo de Negri | ISD - Neri | + 00 | |
| 9 | Enrico Rossi | Ceramica Flaminia | + 00 | |
| 10 | Francisco Pacheco Torres | Xacobeo-Galicia | + 00 | |
| 11 | Daniele Callegarin | CDC - Cavaliere | + 00 | |
| 12 | Domenico Loria | CDC - Cavaliere | + 00 | |
| 13 | Luca Paolini | Acqua e Sapone | + 00 | |
| 14 | Rafaâ Chtioui | Acqua e Sapone | + 00 | |
| 15 | Krzysztof Szczawinski | Miche Pro Cycling Team | + 00 | |
| 16 | Alessandro Bertolini | Androni Giocattoli | + 00 | |
| 17 | John Murphy | BMC Racing Team | + 00 | |
| 18 | Angel Rubiano Miguel | Meridiana - Kamen Team | + 00 | |
| 19 | Jure Kocjan | Carmiooro NGC | + 00 | |
| 20 | Cristian Benenati | De Rosa - Stac Plastic | + 00 | |
| 21 | Ruggero Marzoli | Acqua e Sapone | + 00 | |
| 22 | Murilo Fischer | Garmin-Transitions | + 15 | + 15 |
| 23 | Antonio de Segovia Jose | Xacobeo-Galicia | + 32 | + 32 |
| 24 | Kristjan Fajt | Adria Mobil | + 32 | + 32 |
| 25 | Davide Bonuccelli | CDC - Cavaliere | + 32 | + 32 |
| 26 | Gustavo Dominguez Lemos | Xacobeo-Galicia | + 32 | + 32 |
| 27 | Manuel Ortega | Andalucía - Cajasur | + 32 | + 32 |
| 28 | Fabio Taborre | Androni Giocattoli | + 32 | + 32 |
| 29 | Andrea Pasqualon | Lampre-Farnese Vini | + 32 | + 32 |
| 30 | Giorgio Brambilla | De Rosa - Stac Plastic | + 01:26 | + 01:26 |
| 31 | Fabio Felline | Footon-Servetto | + 01:26 | + 01:26 |
| 32 | Eros Capecchi | Footon-Servetto | + 01:26 | + 01:26 |
| 33 | Marino Palandri | Adria Mobil | + 01:26 | + 01:26 |
| 34 | Vicente Toribio Jose | Andalucía - Cajasur | + 01:26 | + 01:26 |
| 35 | Pablo Torres | Xacobeo-Galicia | + 01:26 | + 01:26 |
| 36 | Florian Stalder | BMC Racing Team | + 01:26 | + 01:26 |
| 37 | Jérôme Baugnies | Topsport Vlaanderen - Mercator | + 01:26 | + 01:26 |
| 38 | Daniele Pietropolli | Lampre-Farnese Vini | + 01:26 | + 01:26 |
| 39 | Martin Kohler | BMC Racing Team | + 01:26 | + 01:26 |
| 40 | Steve Morabito | BMC Racing Team | + 01:26 | + 01:26 |
| 41 | Volodymyr Bileka | Amore & Vita-Conad | + 01:26 | + 01:26 |
| 42 | Angel Vicioso | Andalucía - Cajasur | + 01:26 | + 01:26 |
| 43 | Kristof Vandewalle | Topsport Vlaanderen - Mercator | + 01:26 | + 01:26 |
| 44 | Fredrik Kessiakoff | Garmin-Transitions | + 01:26 | + 01:26 |
| 45 | Daniel Martin | Garmin-Transitions | + 01:26 | + 01:26 |
| 46 | Thomas Peterson | Garmin-Transitions | + 01:26 | + 01:26 |
| 47 | Dario Andriotto | Acqua e Sapone | + 01:26 | + 01:26 |
| 48 | Gianluca Mirenda | ISD - Neri | + 01:26 | + 01:26 |
| 49 | Bartosz Huzarski | ISD - Neri | + 01:26 | + 01:26 |
| 50 | Giovanni Visconti | ISD - Neri | + 01:26 | + 01:26 |
| 51 | Kristjan Koren | Liquigas-Doimo | + 01:26 | + 01:26 |
| 52 | Oleg Berdos | De Rosa - Stac Plastic | + 01:26 | + 01:26 |
| 53 | Edoardo Girardi | Ceramica Flaminia | + 01:26 | + 01:26 |
| 54 | Volodymyr Zagorodniy | Miche Pro Cycling Team | + 01:26 | + 01:26 |
| 55 | Juraj Sagan | Liquigas-Doimo | + 01:26 | + 01:26 |
| 56 | Marcello Pavarin | Colnago - CSF Inox | + 01:26 | + 01:26 |
| 57 | Antonio Piedra | Andalucía - Cajasur | + 01:26 | + 01:26 |
| 58 | Paolo Longo Borghini | ISD - Neri | + 01:26 | + 01:26 |
| 59 | Diego Caccia | ISD - Neri | + 01:26 | + 01:26 |
| 60 | Alessandro Proni | Acqua e Sapone | + 01:26 | + 01:26 |
| 61 | Przemyslaw Niemiec | Miche Pro Cycling Team | + 01:26 | + 01:26 |
| 62 | Sergio Carrasco Garcia | Andalucía - Cajasur | + 01:26 | + 01:26 |
| 63 | Maxime Monfort | Team HTC - Columbia | + 01:26 | + 01:26 |
| 64 | Federico Canuti | Colnago - CSF Inox | + 01:26 | + 01:26 |
| 65 | Alessandro Raisoni | Carmiooro NGC | + 01:26 | + 01:26 |
| 66 | Enrico Magazzini | Lampre-Farnese Vini | + 01:26 | + 01:26 |
| 67 | Alan Marangoni | Colnago - CSF Inox | + 01:26 | + 01:26 |
| 68 | Peter Sagan | Liquigas-Doimo | + 01:26 | + 01:26 |
| 69 | Giampaolo Cheula | Footon-Servetto | + 01:26 | + 01:26 |
| 70 | Ivan Basso | Liquigas-Doimo | + 01:26 | + 01:26 |
| 71 | Manuele Boaro | Carmiooro NGC | + 01:26 | + 01:26 |
| 72 | Vincenzo Nibali | Liquigas-Doimo | + 01:26 | + 01:26 |
| 73 | Marco Frapporti | Colnago - CSF Inox | + 01:26 | + 01:26 |
| 74 | Michele Gaia | Colnago - CSF Inox | + 01:26 | + 01:26 |
| 75 | Marco Cattaneo | De Rosa - Stac Plastic | + 01:26 | + 01:26 |
| 76 | Giairo Ermeti | De Rosa - Stac Plastic | + 01:35 | + 01:35 |
| 77 | Ricardo van der Velde | Garmin-Transitions | + 02:04 | + 02:04 |
| 78 | Fabio Terrenzio | Carmiooro NGC | + 02:04 | + 02:04 |
| 79 | Martin Velits | Team HTC - Columbia | + 02:04 | + 02:04 |
| 80 | Simone Campagnaro | Miche Pro Cycling Team | + 02:04 | + 02:04 |
| 81 | Leonardo Pinizzotto | Miche Pro Cycling Team | + 02:04 | + 02:04 |