| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Boris Shpilevsky | Kio Ene - Tonazzo - DMT | 44:48 | |
| 2 | Dario Cioni | Predictor - Lotto | + 01 | + 01 |
| 3 | Giovanni Visconti | Quick Step - Innergetic | + 13 | + 13 |
| 4 | Vincenzo Nibali | Liquigas | + 19 | + 19 |
| 5 | Vladimir Gusev | Discovery Channel | + 37 | + 37 |
| 6 | Oleksandr Kvachuk | Cinelli - Endeka - OPD | + 01:36 | + 01:36 |
| 7 | Paolo Bettini | Quick Step - Innergetic | + 01:42 | + 01:42 |
| 8 | Alessandro Ballan | Lampre-Fondital | + 01:43 | + 01:43 |
| 9 | Andriy Grivko | Team Milram | + 01:59 | + 01:59 |
| 10 | Fabio Sabatini | Team Milram | + 02:04 | + 02:04 |
| 11 | Roberto Traficante | Team LPR | + 02:06 | + 02:06 |
| 12 | Gabriele Bosisio | Tenax - Menikini | + 02:07 | + 02:07 |
| 13 | Filippo Pozzato | Liquigas | + 02:18 | + 02:18 |
| 14 | Manuele Spadi | Ceramica Flaminia | + 02:49 | + 02:49 |
| 15 | Andrea Tonti | Quick Step - Innergetic | + 03:01 | + 03:01 |
| 16 | Leonardo Scarselli | Quick Step - Innergetic | + 03:21 | + 03:21 |
| 17 | Dmytro Grabovskyy | Quick Step - Innergetic | + 03:25 | + 03:25 |
| 18 | Daniele Righi | Lampre-Fondital | + 03:30 | + 03:30 |
| 19 | Alessandro Bertolini | Serramenti - Selle Italia | + 03:31 | + 03:31 |
| 20 | Luca Sabatino | Tenax - Menikini | + 04:11 | + 04:11 |
| 21 | Slawomyr Bury | Amore & Vita - McDonald's | + 05:26 | + 05:26 |
| 22 | Alessio Signego | OTC Doors - Lauretana | + 05:27 | + 05:27 |
| 23 | Salvatore Commesso | Tinkoff Credit Systems | + 05:53 | + 05:53 |
| 24 | Eros Capecchi | Liquigas | + 06:03 | + 06:03 |
| 25 | Artur Krol | Amore & Vita - McDonald's | + 06:04 | + 06:04 |