| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Óscar Freire | Rabobank | 06:57:28 | |
| 2 | Tom Boonen | Quick Step | + 00 | |
| 3 | Alessandro Petacchi | Lampre-Farnese Vini | + 00 | |
| 4 | Sacha Modolo | Colnago - CSF Inox | + 00 | |
| 5 | Daniele Bennati | Liquigas-Doimo | + 00 | |
| 6 | Thor Hushovd | Cervelo TestTeam | + 00 | |
| 7 | Francesco Ginanni | Androni Giocattoli | + 00 | |
| 8 | Maxim Iglinskiy | Astana | + 00 | |
| 9 | Philippe Gilbert | Omega Pharma-Lotto | + 00 | |
| 10 | Luca Paolini | Acqua e Sapone | + 00 | |
| 11 | Matti Breschel | Team Saxo Bank | + 00 | |
| 12 | Anthony Geslin | Française des Jeux | + 00 | |
| 13 | Enrico Gasparotto | Astana | + 00 | |
| 14 | Geoffroy Lequatre | RadioShack | + 00 | |
| 15 | Paul Martens | Rabobank | + 00 | |
| 16 | Yoann Offredo | Française des Jeux | + 00 | |
| 17 | Fabian Cancellara | Team Saxo Bank | + 00 | |
| 18 | Antonio Flecha Juan | Sky Professional Cycling Team | + 00 | |
| 19 | Linus Gerdemann | Team Milram | + 00 | |
| 20 | Pablo Lastras | Caisse d'Epargne | + 00 | |
| 21 | Sylvain Chavanel | Quick Step | + 00 | |
| 22 | Marcus Burghardt | BMC Racing Team | + 00 | |
| 23 | Daniel Oss | Liquigas-Doimo | + 00 | |
| 24 | Xavier Florencio | Cervelo TestTeam | + 00 | |
| 25 | Michael Rogers | Team HTC - Columbia | + 00 | |
| 26 | Andriy Grivko | Astana | + 09 | + 09 |
| 27 | Thomas Löfkvist | Sky Professional Cycling Team | + 09 | + 09 |
| 28 | Vincenzo Nibali | Liquigas-Doimo | + 09 | + 09 |
| 29 | Filippo Pozzato | Team Katusha | + 18 | + 18 |
| 30 | Michele Scarponi | Androni Giocattoli | + 21 | + 21 |
| 31 | Sergei Ivanov | Team Katusha | + 39 | + 39 |
| 32 | Simon Clarke | ISD - Neri | + 01:35 | + 01:35 |
| 33 | Lorenzo Bernucci | Lampre-Farnese Vini | + 01:35 | + 01:35 |
| 34 | Damiano Cunego | Lampre-Farnese Vini | + 01:35 | + 01:35 |
| 35 | Andreas Klier | Cervelo TestTeam | + 01:35 | + 01:35 |
| 36 | Alan Perez Lezaun | Euskaltel-Euskadi | + 01:40 | + 01:40 |
| 37 | Dmitriy Muravyev | RadioShack | + 01:40 | + 01:40 |
| 38 | Lloyd Mondory | AG2R La Mondiale | + 01:40 | + 01:40 |
| 39 | Joaquin Rojas José | Caisse d'Epargne | + 01:40 | + 01:40 |
| 40 | Francisco Ventoso | Carmiooro NGC | + 01:40 | + 01:40 |
| 41 | Niki Terpstra | Team Milram | + 01:40 | + 01:40 |
| 42 | Tyler Farrar | Garmin-Transitions | + 01:40 | + 01:40 |
| 43 | José Oroz Juan | Euskaltel-Euskadi | + 01:40 | + 01:40 |
| 44 | Bernhard Eisel | Team HTC - Columbia | + 01:40 | + 01:40 |
| 45 | Rubén Pérez Moreno | Euskaltel-Euskadi | + 01:40 | + 01:40 |
| 46 | Alexandr Kolobnev | Team Katusha | + 01:40 | + 01:40 |
| 47 | Greg van Avermaet | Omega Pharma-Lotto | + 01:40 | + 01:40 |
| 48 | Eduard Vorganov | Team Katusha | + 01:40 | + 01:40 |
| 49 | Rui Costa | Caisse d'Epargne | + 01:40 | + 01:40 |
| 50 | Christian Knees | Team Milram | + 01:40 | + 01:40 |
| 51 | Markus Fothen | Team Milram | + 01:40 | + 01:40 |
| 52 | Karsten Kroon | BMC Racing Team | + 01:40 | + 01:40 |
| 53 | Wesley Sulzberger | Française des Jeux | + 01:40 | + 01:40 |
| 54 | Michael Barry | Sky Professional Cycling Team | + 01:40 | + 01:40 |
| 55 | George Hincapie | BMC Racing Team | + 01:40 | + 01:40 |
| 56 | Markel Irizar | RadioShack | + 01:40 | + 01:40 |
| 57 | Sebastien Rosseler | RadioShack | + 01:40 | + 01:40 |
| 58 | Jackson Rodriguez | Androni Giocattoli | + 01:40 | + 01:40 |
| 59 | Gabriel Rasch | Cervelo TestTeam | + 01:40 | + 01:40 |
| 60 | Bram Tankink | Rabobank | + 01:40 | + 01:40 |
| 61 | Nick Nuyens | Rabobank | + 01:40 | + 01:40 |
| 62 | Sebastian Langeveld | Rabobank | + 01:40 | + 01:40 |
| 63 | Oscar Gatto | ISD - Neri | + 01:40 | + 01:40 |
| 64 | Alessandro Ballan | BMC Racing Team | + 01:40 | + 01:40 |
| 65 | Sébastien Hinault | AG2R La Mondiale | + 01:40 | + 01:40 |
| 66 | René Mandri | AG2R La Mondiale | + 01:40 | + 01:40 |
| 67 | William Bonnet | BBOX Bouygues Telecom | + 01:40 | + 01:40 |
| 68 | Matteo Tosatto | Quick Step | + 01:40 | + 01:40 |
| 69 | Emanuele Sella | Carmiooro NGC | + 01:40 | + 01:40 |
| 70 | Francesco Gavazzi | Lampre-Farnese Vini | + 01:40 | + 01:40 |
| 71 | Jérôme Pineau | Quick Step | + 01:40 | + 01:40 |
| 72 | Iñaki Isasi | Euskaltel-Euskadi | + 01:40 | + 01:40 |
| 73 | Domenico Pozzovivo | Colnago - CSF Inox | + 01:40 | + 01:40 |
| 74 | Mirco Lorenzetto | Lampre-Farnese Vini | + 01:40 | + 01:40 |
| 75 | Dries Devenyns | Quick Step | + 01:40 | + 01:40 |
| 76 | José Serpa | Androni Giocattoli | + 01:40 | + 01:40 |
| 77 | Manuele Mori | Lampre-Farnese Vini | + 01:40 | + 01:40 |
| 78 | Assan Bazayev | Astana | + 01:40 | + 01:40 |
| 79 | Manuel Quinziato | Liquigas-Doimo | + 01:40 | + 01:40 |
| 80 | Benoît Vaugrenard | Française des Jeux | + 01:40 | + 01:40 |
| 81 | Rigoberto Uran | Caisse d'Epargne | + 01:40 | + 01:40 |
| 82 | Franco Pellizotti | Liquigas-Doimo | + 01:49 | + 01:49 |
| 83 | Stefano Garzelli | Acqua e Sapone | + 01:49 | + 01:49 |
| 84 | Dimitri Champion | AG2R La Mondiale | + 01:54 | + 01:54 |
| 85 | Francesco Failli | Acqua e Sapone | + 03:04 | + 03:04 |
| 86 | Roman Kreuziger | Liquigas-Doimo | + 03:13 | + 03:13 |
| 87 | Vasil Kiryienka | Caisse d'Epargne | + 03:13 | + 03:13 |
| 88 | Steve Chainel | BBOX Bouygues Telecom | + 05:29 | + 05:29 |
| 89 | Mark Cavendish | Team HTC - Columbia | + 06:12 | + 06:12 |
| 90 | Mirko Selvaggi | Astana | + 06:12 | + 06:12 |
| 91 | Giovanni Visconti | ISD - Neri | + 06:12 | + 06:12 |
| 92 | Gregory Rast | RadioShack | + 06:12 | + 06:12 |
| 93 | Tom Leezer | Rabobank | + 06:12 | + 06:12 |
| 94 | Lars Boom | Rabobank | + 06:12 | + 06:12 |
| 95 | Maarten Tjallingii | Rabobank | + 06:12 | + 06:12 |
| 96 | Greg Henderson | Sky Professional Cycling Team | + 06:12 | + 06:12 |
| 97 | Brett Lancaster | Cervelo TestTeam | + 06:12 | + 06:12 |
| 98 | Johan Vansummeren | Garmin-Transitions | + 06:12 | + 06:12 |
| 99 | Chris Sutton | Sky Professional Cycling Team | + 06:12 | + 06:12 |
| 100 | Cameron Wurf | Androni Giocattoli | + 06:12 | + 06:12 |
| 101 | Jürgen Roelandts | Omega Pharma-Lotto | + 06:12 | + 06:12 |
| 102 | Frédéric Guesdon | Française des Jeux | + 06:12 | + 06:12 |
| 103 | Mickaël Delage | Omega Pharma-Lotto | + 06:12 | + 06:12 |
| 104 | Anders Lund | Team Saxo Bank | + 06:12 | + 06:12 |
| 105 | David Millar | Garmin-Transitions | + 06:12 | + 06:12 |
| 106 | Edvald Boasson-hagen | Sky Professional Cycling Team | + 06:12 | + 06:12 |
| 107 | Marco Velo | Quick Step | + 06:12 | + 06:12 |
| 108 | Xabier Zandio | Caisse d'Epargne | + 06:12 | + 06:12 |
| 109 | Sébastien Turgot | BBOX Bouygues Telecom | + 10:07 | + 10:07 |
| 110 | Blel Kadri | AG2R La Mondiale | + 10:07 | + 10:07 |
| 111 | Luca Mazzanti | Team Katusha | + 10:07 | + 10:07 |
| 112 | Daryl Impey | RadioShack | + 10:07 | + 10:07 |
| 113 | Fumiyuki Beppu | RadioShack | + 10:07 | + 10:07 |
| 114 | Michael Schär | BMC Racing Team | + 10:07 | + 10:07 |
| 115 | Peter Wrolich | Team Milram | + 10:07 | + 10:07 |
| 116 | Alan Marangoni | Colnago - CSF Inox | + 10:07 | + 10:07 |
| 117 | Marco Frapporti | Colnago - CSF Inox | + 10:07 | + 10:07 |
| 118 | Vladimir Efimkin | AG2R La Mondiale | + 10:07 | + 10:07 |
| 119 | Mathieu Claude | BBOX Bouygues Telecom | + 10:07 | + 10:07 |
| 120 | Stuart O'grady | Team Saxo Bank | + 10:07 | + 10:07 |
| 121 | Lars Bak | Team HTC - Columbia | + 10:07 | + 10:07 |
| 122 | Matthew Wilson | Garmin-Transitions | + 10:07 | + 10:07 |
| 123 | Paul Voss | Team Milram | + 10:07 | + 10:07 |
| 124 | Danilo Hondo | Lampre-Farnese Vini | + 10:07 | + 10:07 |
| 125 | Staf Scheirlinckx | Omega Pharma-Lotto | + 10:07 | + 10:07 |
| 126 | Allan Davis | Astana | + 10:07 | + 10:07 |
| 127 | Frank Høj | Team Saxo Bank | + 10:07 | + 10:07 |
| 128 | Gustav Larsson | Team Saxo Bank | + 10:07 | + 10:07 |
| 129 | Mathew Hayman | Sky Professional Cycling Team | + 10:07 | + 10:07 |
| 130 | Dmytro Grabovskyy | ISD - Neri | + 10:07 | + 10:07 |
| 131 | Paolo Longo Borghini | ISD - Neri | + 10:07 | + 10:07 |
| 132 | Aristide Ratti | Carmiooro NGC | + 10:07 | + 10:07 |
| 133 | Jure Kocjan | Carmiooro NGC | + 10:07 | + 10:07 |
| 134 | Vladimir Miholjevic | Acqua e Sapone | + 10:07 | + 10:07 |
| 135 | Alessandro Fantini | Acqua e Sapone | + 10:07 | + 10:07 |
| 136 | Pablo Urtasun | Euskaltel-Euskadi | + 10:07 | + 10:07 |
| 137 | Jorge Azanza | Euskaltel-Euskadi | + 10:07 | + 10:07 |
| 138 | Laurent Beuret | Carmiooro NGC | + 10:07 | + 10:07 |
| 139 | Rubens Bertogliati | Androni Giocattoli | + 10:07 | + 10:07 |
| 140 | Eric Berthou | Carmiooro NGC | + 10:07 | + 10:07 |
| 141 | Luke Roberts | Team Milram | + 10:18 | + 10:18 |
| 142 | Jeremy Hunt | Cervelo TestTeam | + 14:24 | + 14:24 |
| 143 | Olivier Bonnaire | Française des Jeux | + 14:24 | + 14:24 |
| 144 | Alessandro Donati | Acqua e Sapone | + 14:24 | + 14:24 |
| 145 | Valeriy Dmitriyev | Astana | + 17:17 | + 17:17 |
| 146 | Danilo Wyss | BMC Racing Team | + 17:17 | + 17:17 |
| 147 | Roy Sentjens | Team Milram | + 17:17 | + 17:17 |
| 148 | Valerio Agnoli | Liquigas-Doimo | + 17:17 | + 17:17 |
| 149 | Yauheni Hutarovich | Française des Jeux | + 17:17 | + 17:17 |
| 150 | Diego Caccia | ISD - Neri | + 17:17 | + 17:17 |
| 151 | Daniele Ratto | Carmiooro NGC | + 17:17 | + 17:17 |
| 152 | Maxime Bouet | AG2R La Mondiale | + 17:17 | + 17:17 |
| 153 | Fabrice Piemontesi | Androni Giocattoli | + 17:17 | + 17:17 |