| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Sergio Marinangeli | Naturino - Sapore di Mare | 04:43:45 | |
| 2 | Ruggero Marzoli | + 00 | ||
| 3 | Riccardo Riccò | Saunier Duval - Prodir | + 00 | |
| 4 | Danilo Hondo | Team Lamonta | + 00 | |
| 5 | Manuele Mori | Saunier Duval - Prodir | + 00 | |
| 6 | Paolo Bailetti | Team 3C Casalinghi Jet | + 00 | |
| 7 | Daniele Callegarin | Team 3C Casalinghi Jet | + 00 | |
| 8 | Mikhaylo Khalilov | Team LPR | + 00 | |
| 9 | Raivis Belohvosciks | CB Immobiliare - Universal | + 00 | |
| 10 | Daniele Pietropolli | Team Tenax Salmilano | + 00 | |
| 11 | Błażej Janiaczyk | Team 3C Casalinghi Jet | + 00 | |
| 12 | Kjell Carlström | Liquigas | + 00 | |
| 13 | Lorenzo Cardellini | CB Immobiliare - Universal | + 00 | |
| 14 | Mirco Lorenzetto | Team Milram | + 00 | |
| 15 | Matteo Carrara | Lampre-Fondital | + 00 | |
| 16 | Matteo Tosatto | Quick Step - Innergetic | + 00 | |
| 17 | Hubert Schwab | Quick Step - Innergetic | + 00 | |
| 18 | Andrea Moletta | Gerolsteiner | + 00 | |
| 19 | Alexandr Kolobnev | Rabobank | + 00 | |
| 20 | Mauricio Ardila | Rabobank | + 00 | |
| 21 | Mikel Astarloza | AG2R Prévoyance | + 00 | |
| 22 | Manuele Spadi | Ceramica Flaminia | + 00 | |
| 23 | Hugo Sabido | Barloworld | + 00 | |
| 24 | Pieter Weening | Rabobank | + 00 | |
| 25 | Theo Eltink | Rabobank | + 00 | |
| 26 | David Mcpartland | Team Tenax Salmilano | + 00 | |
| 27 | Nicki Sørensen | Team CSC | + 00 | |
| 28 | Alberto Milani | CB Immobiliare - Universal | + 00 | |
| 29 | Oscar Gatto | Team LPR | + 00 | |
| 30 | Roman Kreuziger | Liquigas | + 00 | |
| 31 | Konstantin Klyuev | Team 3C Casalinghi Jet | + 00 | |
| 32 | Evgeni Petrov | Lampre-Fondital | + 00 | |
| 33 | Giuseppe Di Grande | Miche | + 00 | |
| 34 | Brian Vandborg | Team CSC | + 00 | |
| 35 | Fabio Gilioli | CB Immobiliare - Universal | + 00 | |
| 36 | Dmitri Nikandrov | CB Immobiliare - Universal | + 00 | |
| 37 | Massimiliano Maisto | CB Immobiliare - Universal | + 00 | |
| 38 | Dario Cioni | Liquigas | + 00 | |
| 39 | Vincenzo Nibali | Liquigas | + 00 | |
| 40 | Alexander Efimkin | Barloworld | + 00 | |
| 41 | Cédric Vasseur | Quick Step - Innergetic | + 00 | |
| 42 | Alexander Arekeev | Acqua e Sapone - Caffe Mokambo | + 00 | |
| 43 | Andrea Peron | Team CSC | + 00 | |
| 44 | Georg Totschnig | Gerolsteiner | + 00 | |
| 45 | Volodymir Gustov | Team CSC | + 00 | |
| 46 | Sergio Barbero | Selle Italia - Serramenti | + 00 | |
| 47 | Paride Grillo | Ceramica Panaria - Navigare | + 00 | |
| 48 | Fränk Schleck | Team CSC | + 00 | |
| 49 | Luca Mazzanti | Ceramica Panaria - Navigare | + 00 | |
| 50 | Luca Solari | Team 3C Casalinghi Jet | + 00 | |
| 51 | Niklas Axelsson | Selle Italia - Serramenti | + 00 | |
| 52 | Przemyslaw Niemiec | Miche | + 00 | |
| 53 | Alexandre Bazhenov | Naturino - Sapore di Mare | + 00 | |
| 54 | Eddy Ratti | Naturino - Sapore di Mare | + 00 | |
| 55 | Andrea Noè | Liquigas | + 00 | |
| 56 | Paolo Bettini | Quick Step - Innergetic | + 32 | + 32 |
| 57 | Domenico Pozzovivo | Ceramica Panaria - Navigare | + 01:12 | + 01:12 |
| 58 | Domenico Agosta | Selle Italia - Serramenti | + 02:25 | + 02:25 |
| 59 | Íñigo Cuesta | Team CSC | + 02:25 | + 02:25 |
| 60 | Tomasz Marczynski | Ceramica Flaminia | + 02:25 | + 02:25 |
| 61 | Inigo Chaurreau | AG2R Prévoyance | + 02:25 | + 02:25 |
| 62 | Philippe Schnyder | Selle Italia - Serramenti | + 02:25 | + 02:25 |
| 63 | Paolo Longo Borghini | Ceramica Flaminia | + 02:25 | + 02:25 |
| 64 | Luis Laverde | Ceramica Panaria - Navigare | + 02:25 | + 02:25 |
| 65 | Alessandro Bertolini | Selle Italia - Serramenti | + 02:25 | + 02:25 |
| 66 | Philip Deignan | AG2R Prévoyance | + 02:25 | + 02:25 |
| 67 | Sylwester Szmyd | Lampre-Fondital | + 02:25 | + 02:25 |
| 68 | Oleksandr Kvachuk | Team 3C Casalinghi Jet | + 02:25 | + 02:25 |
| 69 | Raffaele Ferrara | Team 3C Casalinghi Jet | + 02:25 | + 02:25 |
| 70 | William Walker | Rabobank CT | + 02:25 | + 02:25 |
| 71 | Gabriele Bosisio | Team Tenax Salmilano | + 02:25 | + 02:25 |
| 72 | Crescenzo D'amore | Acqua e Sapone - Caffe Mokambo | + 02:25 | + 02:25 |
| 73 | Ruslan Pidgornyy | Team Tenax Salmilano | + 02:25 | + 02:25 |
| 74 | Roberto Ferrari | Team Tenax Salmilano | + 02:25 | + 02:25 |
| 75 | Francesco Chicchi | Quick Step - Innergetic | + 04:12 | + 04:12 |
| 76 | Ivan Santaromita | Quick Step - Innergetic | + 04:12 | + 04:12 |
| 77 | Hannes Blank | Team Lamonta | + 04:12 | + 04:12 |
| 78 | Leonardo Giordani | Naturino - Sapore di Mare | + 04:12 | + 04:12 |
| 79 | Luca Pierfelici | Naturino - Sapore di Mare | + 04:12 | + 04:12 |
| 80 | Timothy Jones | Amore & Vita - McDonald's | + 04:12 | + 04:12 |
| 81 | Hubert Krys | Ceramica Flaminia | + 04:12 | + 04:12 |
| 82 | Rino Zampilli | Naturino - Sapore di Mare | + 04:12 | + 04:12 |
| 83 | Pasquale Muto | Miche | + 04:12 | + 04:12 |
| 84 | Rubens Bertogliati | Saunier Duval - Prodir | + 04:12 | + 04:12 |
| 85 | Diego Genovesi | OTC Doors - Lauretana | + 04:12 | + 04:12 |
| 86 | Yuriy Metlushenko | Team LPR | + 04:12 | + 04:12 |
| 87 | Alessandro Bertuola | Team Tenax Salmilano | + 04:12 | + 04:12 |
| 88 | Oliver Zaugg | Saunier Duval - Prodir | + 04:12 | + 04:12 |
| 89 | Fabian Wegmann | Gerolsteiner | + 04:12 | + 04:12 |
| 90 | Marco Marzano | Lampre-Fondital | + 04:12 | + 04:12 |
| 91 | Johannes Fröhlinger | Gerolsteiner | + 04:12 | + 04:12 |
| 92 | Donato Cannone | CB Immobiliare - Universal | + 04:12 | + 04:12 |
| 93 | Dainius Kairelis | Amore & Vita - McDonald's | + 04:12 | + 04:12 |
| 94 | Alberto Castaneda Jaime | Lampre-Fondital | + 04:12 | + 04:12 |
| 95 | Vladimir Duma | CB Immobiliare - Universal | + 04:12 | + 04:12 |
| 96 | Matej Mugerli | Liquigas | + 07:12 | + 07:12 |