| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Francisco Ventoso | Carmiooro - A-Style | 04:12:08 | |
| 2 | Giovanni Visconti | ISD - Neri | + 00 | |
| 3 | Enrico Rossi | Ceramica Flaminia-Bossini Docce | + 00 | |
| 4 | Luca Paolini | Acqua e Sapone - Caffe Mokambo | + 00 | |
| 5 | Dario Cataldo | Quick Step | + 00 | |
| 6 | Luca Mazzanti | Team Katusha | + 00 | |
| 7 | Krzysztof Szczawinski | Miche - Silver Cross | + 00 | |
| 8 | Andrea Moletta | Miche - Silver Cross | + 00 | |
| 9 | Damiano Cunego | Lampre-NGC | + 00 | |
| 10 | Daniele Callegarin | Centri della Calzatura | + 00 | |
| 11 | Marcello Pavarin | CSF Group - Navigare | + 00 | |
| 12 | Paolo Longo Borghini | Barloworld - Bianchi | + 00 | |
| 13 | Craig Lewis | Team Columbia - HTC | + 00 | |
| 14 | Fabian Wegmann | Team Milram | + 00 | |
| 15 | Fabrice Piemontesi | Team Piemonte | + 00 | |
| 16 | Kim Kirchen | Team Columbia - HTC | + 00 | |
| 17 | Nikita Eskov | Team Katusha | + 00 | |
| 18 | Volodymyr Starchyk | Amore & Vita - McDonald's | + 00 | |
| 19 | Davide Malacarne | Quick Step | + 00 | |
| 20 | Bauke Mollema | Rabobank | + 00 | |
| 21 | Grischa Niermann | Rabobank | + 00 | |
| 22 | Hubert Schwab | Quick Step | + 00 | |
| 23 | Paul Martens | Rabobank | + 00 | |
| 24 | Tadej Valjavec | AG2R La Mondiale | + 00 | |
| 25 | Diego Caccia | Barloworld - Bianchi | + 00 | |
| 26 | Matthew Lloyd | Silence - Lotto | + 00 | |
| 27 | Alexandr Kolobnev | Team Saxo Bank | + 00 | |
| 28 | Christophe Riblon | AG2R La Mondiale | + 00 | |
| 29 | Ludovic Turpin | AG2R La Mondiale | + 00 | |
| 30 | Yannick Eijssen | Silence - Lotto | + 00 | |
| 31 | Eugenio Loria | Centri della Calzatura | + 00 | |
| 32 | Maxime Monfort | Team Columbia - HTC | + 00 | |
| 33 | Serguei Klimov | Team Katusha | + 00 | |
| 34 | Alexandre Botcharov | Team Katusha | + 00 | |
| 35 | Eros Capecchi | Fuji-Servetto | + 00 | |
| 36 | Daniele Colli | Carmiooro - A-Style | + 00 | |
| 37 | Brian Vandborg | Liquigas | + 00 | |
| 38 | Alan Marangoni | CSF Group - Navigare | + 00 | |
| 39 | Anker Sørensen Chris | Team Saxo Bank | + 00 | |
| 40 | Alberto Fernandez | Fuji-Servetto | + 00 | |
| 41 | Gustav Larsson | Team Saxo Bank | + 00 | |
| 42 | Bolat Raimbekov | Astana | + 00 | |
| 43 | Thomas Löfkvist | Team Columbia - HTC | + 00 | |
| 44 | Carlos Barredo | Quick Step | + 00 | |
| 45 | Johannes Fröhlinger | Team Milram | + 00 | |
| 46 | Peter Velits | Team Milram | + 00 | |
| 47 | Pavel Brutt | Team Katusha | + 00 | |
| 48 | Manuele Mori | Lampre-NGC | + 00 | |
| 49 | Massimo Codol | Acqua e Sapone - Caffe Mokambo | + 00 | |
| 50 | Evgeni Petrov | Team Katusha | + 00 | |
| 51 | Simon Clarke | ISD - Neri | + 00 | |
| 52 | Domenico Pozzovivo | CSF Group - Navigare | + 00 | |
| 53 | Leonardo Giordani | Ceramica Flaminia-Bossini Docce | + 00 | |
| 54 | Marco Pinotti | Team Columbia - HTC | + 00 | |
| 55 | Leonardo Bertagnolli | Serramenti - Androni Giocattoli | + 00 | |
| 56 | Chris Horner | Astana | + 00 | |
| 57 | Alexandre Vinokourov | Astana | + 27 | + 27 |
| 58 | Maciej Paterski | Liquigas | + 01:56 | + 01:56 |
| 59 | Enrico Magazzini | Lampre-NGC | + 01:56 | + 01:56 |
| 60 | Tomasz Marczynski | Miche - Silver Cross | + 01:56 | + 01:56 |
| 61 | Roman Kireyev | Astana | + 01:56 | + 01:56 |
| 62 | Kanstantsin Sivtsov | Team Columbia - HTC | + 01:56 | + 01:56 |
| 63 | Carlo Scognamiglio | Barloworld - Bianchi | + 01:56 | + 01:56 |
| 64 | Alessandro Donati | Acqua e Sapone - Caffe Mokambo | + 01:56 | + 01:56 |
| 65 | Dmitriy Kozontchuk | Rabobank | + 01:56 | + 01:56 |
| 66 | Vladimir Efimkin | AG2R La Mondiale | + 01:56 | + 01:56 |
| 67 | Francesco Ginanni | Serramenti - Androni Giocattoli | + 01:56 | + 01:56 |
| 68 | Nicolas Roche | AG2R La Mondiale | + 01:56 | + 01:56 |
| 69 | Bram Tankink | Rabobank | + 01:56 | + 01:56 |
| 70 | Laurens ten Dam | Rabobank | + 01:56 | + 01:56 |
| 71 | Murilo Fischer | Liquigas | + 01:56 | + 01:56 |
| 72 | Thomas Bertolini | Serramenti - Androni Giocattoli | + 01:56 | + 01:56 |
| 73 | Artem Ovechkin | Team Katusha | + 02:15 | + 02:15 |
| 74 | Yauhen Sobal | Centri della Calzatura | + 09:38 | + 09:38 |
| 75 | Cesare Benedetti | Liquigas | + 09:38 | + 09:38 |
| 76 | Vladislav Borisov | Amore & Vita - McDonald's | + 09:38 | + 09:38 |
| 77 | Philipp Mamos | Amore & Vita - McDonald's | + 09:38 | + 09:38 |
| 78 | Thomas Kvist | Quick Step | + 09:38 | + 09:38 |
| 79 | Charles Wegelius | Silence - Lotto | + 09:38 | + 09:38 |
| 80 | Roberto Ferrari | LPR Brakes - Farnese Vini | + 09:38 | + 09:38 |
| 81 | Lars Boom | Rabobank | + 09:38 | + 09:38 |
| 82 | Julien Loubet | AG2R La Mondiale | + 09:38 | + 09:38 |
| 83 | Matteo Montaguti | LPR Brakes - Farnese Vini | + 09:38 | + 09:38 |