| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Harold Tejada | Astana Qazaqstan Team | 04:02:07 | |
| 2 | Andrea Piccolo | EF Education-EasyPost | + 00 | |
| 3 | Oscar Sevilla | Team Medellin | + 00 | |
| 4 | Rodrigo Contreras | Nu Colombia | + 00 | |
| 5 | Robinson Chalapud | Team Banco Guayaquil-Bianchi | + 00 | |
| 6 | Andres Pinzon Edgar | GW Erco Shimano | + 00 | |
| 7 | Yeison Reyes | Team Sistecredito | + 00 | |
| 8 | David Guavita Cesar | Colombia Potencia de la Vida-Strongman | + 00 | |
| 9 | Martin López | Astana Qazaqstan Team | + 23 | + 23 |
| 10 | Brandon Rivera | Colombia | + 23 | + 23 |
| 11 | Alejandro Osorio | GW Erco Shimano | + 23 | + 23 |
| 12 | Yeisson Casallas | Colombia Potencia de la Vida-Strongman | + 23 | + 23 |
| 13 | Nairo Quintana | Movistar Team | + 23 | + 23 |
| 14 | Esteban Chaves | EF Education-EasyPost | + 23 | + 23 |
| 15 | Egan Bernal | Colombia | + 23 | + 23 |
| 16 | Jonathan Caicedo | Petrolike | + 23 | + 23 |
| 17 | Richard Carapaz | EF Education-EasyPost | + 23 | + 23 |
| 18 | Diego Pescador | GW Erco Shimano | + 23 | + 23 |
| 19 | Cristian Munoz | Nu Colombia | + 23 | + 23 |
| 20 | Alexander Cepeda | EF Education-EasyPost | + 23 | + 23 |
| 21 | Alexey Lutsenko | Astana Qazaqstan Team | + 23 | + 23 |
| 22 | Rigoberto Uran | EF Education-EasyPost | + 23 | + 23 |
| 23 | Diego Camargo | Petrolike | + 23 | + 23 |
| 24 | Sergio Henao | Nu Colombia | + 23 | + 23 |
| 25 | Daniel Mendez | Nu Colombia | + 23 | + 23 |
| 26 | Aaron van der Beken | Bingoal WB | + 23 | + 23 |
| 27 | Javier Jamaica | Team Medellin | + 23 | + 23 |
| 28 | Byron Guama | Ecuador | + 23 | + 23 |
| 29 | Ramon Muniz Jose | Petrolike | + 38 | + 38 |
| 30 | Efren Santos | Canel's-Java | + 43 | + 43 |
| 31 | Diego Alba Juan | Colombia | + 43 | + 43 |
| 32 | Adrián Bustamante | GW Erco Shimano | + 50 | + 50 |
| 33 | David Castillo Kevin | Team Sistecredito | + 50 | + 50 |
| 34 | Paulo Pantoja | Orgullo Paisa | + 01:00 | + 01:00 |
| 35 | Edgar Cadena | Petrolike | + 01:00 | + 01:00 |
| 36 | Alexander Gil | Orgullo Paisa | + 01:00 | + 01:00 |
| 37 | Steven Haro | Ecuador | + 01:00 | + 01:00 |
| 38 | Daniel Muñoz | Nu Colombia | + 01:00 | + 01:00 |
| 39 | Santiago Montenegro | Ecuador | + 01:00 | + 01:00 |
| 40 | Aldemar Reyes | Team Banco Guayaquil-Bianchi | + 01:00 | + 01:00 |
| 41 | Ivan Sosa | Movistar Team | + 01:00 | + 01:00 |
| 42 | Wilmar Paredes | Team Medellin | + 01:00 | + 01:00 |
| 43 | Lorenzo Quartucci | Team corratec - Vini Fantini | + 01:18 | + 01:18 |
| 44 | Sebastian Castano | Team Sistecredito | + 01:18 | + 01:18 |
| 45 | Julian Cardona Tabares | Team Medellin | + 01:18 | + 01:18 |
| 46 | Camilo Tobar Cristian | Team Saitel | + 01:18 | + 01:18 |
| 47 | Danny Osorio | Orgullo Paisa | + 01:27 | + 01:27 |
| 48 | Oscar Quiroz | Nu Colombia | + 01:38 | + 01:38 |
| 49 | Richard Huera | Ecuador | + 01:38 | + 01:38 |
| 50 | Lennert Teugels | Bingoal WB | + 01:38 | + 01:38 |
| 51 | Diego Guerrero | Team Sistecredito | + 01:38 | + 01:38 |
| 52 | Miguel Florez | Team Banco Guayaquil-Bianchi | + 01:41 | + 01:41 |
| 53 | Hugo Rodriguez | Colombia | + 01:42 | + 01:42 |
| 54 | Michiel Lambrecht | Bingoal WB | + 01:49 | + 01:49 |
| 55 | Rait Ärm | Estonia | + 01:50 | + 01:50 |
| 56 | Ivan Ojeda | Colombia Potencia de la Vida-Strongman | + 01:50 | + 01:50 |
| 57 | Lars Quaedvlieg | Universe Cycling Team | + 01:50 | + 01:50 |
| 58 | Nicolas Sessler | Brazil | + 01:50 | + 01:50 |
| 59 | Luis Mora | Venezuela | + 01:50 | + 01:50 |
| 60 | Alexis Quinteros | Team Banco Guayaquil-Bianchi | + 01:50 | + 01:50 |
| 61 | Franklin Archibold | Panamá es Cultura y Valores | + 01:50 | + 01:50 |
| 62 | Sebastian Caicedo | Team Banco Guayaquil-Bianchi | + 01:50 | + 01:50 |
| 63 | Jose Hernandez | Orgullo Paisa | + 01:50 | + 01:50 |
| 64 | Kenny Nijssen | Universe Cycling Team | + 01:50 | + 01:50 |
| 65 | Franklin Revelo | Team Saitel | + 01:50 | + 01:50 |
| 66 | Milan Lanhove | Bingoal WB | + 02:10 | + 02:10 |
| 67 | Freddy Avila | Colombia Potencia de la Vida-Strongman | + 02:10 | + 02:10 |
| 68 | Brayan Obando | Ecuador | + 02:14 | + 02:14 |
| 69 | Brandon Rojas | GW Erco Shimano | + 02:14 | + 02:14 |
| 70 | Vladimir Lopez | Sidi Ali-Unlock Team | + 02:14 | + 02:14 |
| 71 | Andres Parra Carlos | Team Saitel | + 02:14 | + 02:14 |
| 72 | Davide Baldaccini | Team corratec - Vini Fantini | + 02:14 | + 02:14 |
| 73 | Heiner Parra | Canel's-Java | + 02:14 | + 02:14 |
| 74 | Fabio Duarte | Team Medellin | + 02:14 | + 02:14 |
| 75 | Victor Cesar de Paula | Brazil | + 02:32 | + 02:32 |
| 76 | Pedro Rossi Joao | SwiftCarbon Pro Cycling Brasil | + 02:48 | + 02:48 |
| 77 | Santiago Gomez Marin | Team Sistecredito | + 02:48 | + 02:48 |
| 78 | Jip Steman | Universe Cycling Team | + 03:02 | + 03:02 |
| 79 | Jeison Rujano | Venezuela | + 03:02 | + 03:02 |
| 80 | Erik Caiza | Team Saitel | + 03:02 | + 03:02 |
| 81 | Magno Rabelo Euller | Brazil | + 03:02 | + 03:02 |
| 82 | Dario Gomez Joya Hernan | Sidi Ali-Unlock Team | + 03:02 | + 03:02 |
| 83 | Brayan Sanchez | Team Medellin | + 03:02 | + 03:02 |
| 84 | Jhojan Garcia | Colombia Potencia de la Vida-Strongman | + 03:02 | + 03:02 |
| 85 | Kamal Mahroug | Sidi Ali-Unlock Team | + 03:02 | + 03:02 |
| 86 | Dimitri Peyskens | Bingoal WB | + 03:02 | + 03:02 |
| 87 | Niccolo Bonifazio | Team corratec - Vini Fantini | + 03:02 | + 03:02 |
| 88 | Laureano Rosas | SwiftCarbon Pro Cycling Brasil | + 03:24 | + 03:24 |
| 89 | Cees Bol | Astana Qazaqstan Team | + 04:22 | + 04:22 |
| 90 | Bart Buijk | Universe Cycling Team | + 04:22 | + 04:22 |
| 91 | Markus Pajur | Estonia | + 04:22 | + 04:22 |
| 92 | Jhonatan Restrepo | Colombia | + 04:31 | + 04:31 |
| 93 | Nelson Soto | Petrolike | + 04:45 | + 04:45 |
| 94 | Daniel Arroyave | GW Erco Shimano | + 04:45 | + 04:45 |
| 95 | Tito Rendon Juan | Orgullo Paisa | + 04:45 | + 04:45 |
| 96 | Felipe Chan Romero Hassan | Panamá es Cultura y Valores | + 05:02 | + 05:02 |
| 97 | Ignacio Prado | Canel's-Java | + 05:03 | + 05:03 |
| 98 | Bolivar Espinosa | Panamá es Cultura y Valores | + 06:08 | + 06:08 |
| 99 | Vinicius Rangel | Movistar Team | + 07:10 | + 07:10 |
| 100 | Lauro Chaman | SwiftCarbon Pro Cycling Brasil | + 07:10 | + 07:10 |
| 101 | Davide Persico | Bingoal WB | + 07:10 | + 07:10 |
| 102 | Albert Torres | Movistar Team | + 07:10 | + 07:10 |
| 103 | Andre Gohr | Brazil | + 07:10 | + 07:10 |
| 104 | Marlon Diágama | Colombia Potencia de la Vida-Strongman | + 07:10 | + 07:10 |
| 105 | Rick Nobel | Universe Cycling Team | + 07:10 | + 07:10 |
| 106 | Mark Stewart | Team corratec - Vini Fantini | + 07:10 | + 07:10 |
| 107 | Pablo Alarcon | Canel's-Java | + 07:10 | + 07:10 |
| 108 | Luis Pinto | Venezuela | + 07:10 | + 07:10 |
| 109 | Randish Lorenzo | Panamá es Cultura y Valores | + 07:10 | + 07:10 |
| 110 | Andrey André | Brazil | + 07:10 | + 07:10 |
| 111 | Diego Ochoa | Team Saitel | + 07:10 | + 07:10 |
| 112 | Kevin Jimenez Tepetl | Canel's-Java | + 07:10 | + 07:10 |
| 113 | Cristofer Jurado | Panamá es Cultura y Valores | + 07:10 | + 07:10 |
| 114 | Johan Colon | Orgullo Paisa | + 07:29 | + 07:29 |
| 115 | Diego Hoyos Juan | Team Sistecredito | + 07:29 | + 07:29 |
| 116 | David Villareal | Ecuador | + 08:37 | + 08:37 |
| 117 | Martin Laas | Estonia | + 09:11 | + 09:11 |
| 118 | Norman Vahtra | Estonia | + 09:11 | + 09:11 |
| 119 | Attilio Viviani | Team corratec - Vini Fantini | + 14:31 | + 14:31 |
| 120 | Manuel Aramayo Jose | SwiftCarbon Pro Cycling Brasil | + 14:31 | + 14:31 |
| 121 | Alejandro Gainza Rodriguez | Sidi Ali-Unlock Team | + 14:31 | + 14:31 |
| 122 | Martti Lenzius | Estonia | + 14:31 | + 14:31 |
| 123 | Bernardo Suaza | Petrolike | + 14:31 | + 14:31 |
| 124 | Giulio Masotto | Team corratec - Vini Fantini | + 14:31 | + 14:31 |
| 125 | Cristian Pita | Team Banco Guayaquil-Bianchi | + 14:31 | + 14:31 |
| 126 | Fernando Gaviria | Movistar Team | + 14:31 | + 14:31 |
| 127 | Alejandro Pita Hidrobo | Team Saitel | + 20:11 | + 20:11 |
| 128 | Enmanuel Viloria | Venezuela | + 26:11 | + 26:11 |
| 129 | Nikolaos Zeglis | Sidi Ali-Unlock Team | + 26:11 | + 26:11 |
| 130 | Lauri Tamm | Estonia | + 26:11 | + 26:11 |
| 131 | Sergio Samitier | Movistar Team | + 26:11 | + 26:11 |
| 132 | Alex Strah | Panamá es Cultura y Valores | + 26:11 | + 26:11 |
| 133 | Cormac Mcgeough | Canel's-Java | + 26:11 | + 26:11 |
| 134 | Alessandro Guimaraes | SwiftCarbon Pro Cycling Brasil | + 26:11 | + 26:11 |
| 135 | Tom Wijfje | Universe Cycling Team | + 26:11 | + 26:11 |
| 136 | Michael Mørkøv | Astana Qazaqstan Team | + 26:50 | + 26:50 |
| 137 | Mark Cavendish | Astana Qazaqstan Team | + 26:50 | + 26:50 |
| 138 | Jhonny Araujo | Venezuela | + 26:50 | + 26:50 |