| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Jhonatan Restrepo | Colombia | 03:06:24 | |
| 2 | Richard Carapaz | EF Education-EasyPost | + 00 | |
| 3 | Alejandro Osorio | GW Erco Shimano | + 00 | |
| 4 | Jonathan Caicedo | Petrolike | + 00 | |
| 5 | Harold Tejada | Astana Qazaqstan Team | + 00 | |
| 6 | Martin López | Astana Qazaqstan Team | + 00 | |
| 7 | Yeison Reyes | Team Sistecredito | + 00 | |
| 8 | Rodrigo Contreras | Nu Colombia | + 00 | |
| 9 | Rigoberto Uran | EF Education-EasyPost | + 00 | |
| 10 | Daniel Mendez | Nu Colombia | + 00 | |
| 11 | Ivan Sosa | Movistar Team | + 00 | |
| 12 | Brandon Rivera | Colombia | + 05 | + 05 |
| 13 | Egan Bernal | Colombia | + 09 | + 09 |
| 14 | Cees Bol | Astana Qazaqstan Team | + 13 | + 13 |
| 15 | Brandon Rojas | GW Erco Shimano | + 15 | + 15 |
| 16 | Diego Pescador | GW Erco Shimano | + 15 | + 15 |
| 17 | Wilmar Paredes | Team Medellin | + 15 | + 15 |
| 18 | David Castillo Kevin | Team Sistecredito | + 15 | + 15 |
| 19 | Esteban Chaves | EF Education-EasyPost | + 15 | + 15 |
| 20 | Adrián Bustamante | GW Erco Shimano | + 20 | + 20 |
| 21 | Aldemar Reyes | Team Banco Guayaquil-Bianchi | + 01:09 | + 01:09 |
| 22 | Javier Jamaica | Team Medellin | + 01:09 | + 01:09 |
| 23 | Alexander Cepeda | EF Education-EasyPost | + 01:09 | + 01:09 |
| 24 | Niccolo Bonifazio | Team corratec - Vini Fantini | + 01:22 | + 01:22 |
| 25 | Sergio Samitier | Movistar Team | + 01:22 | + 01:22 |
| 26 | Nairo Quintana | Movistar Team | + 01:22 | + 01:22 |
| 27 | Albert Torres | Movistar Team | + 01:56 | + 01:56 |
| 28 | Aaron van der Beken | Bingoal WB | + 01:56 | + 01:56 |
| 29 | Vinicius Rangel | Movistar Team | + 01:57 | + 01:57 |
| 30 | Andres Pinzon Edgar | GW Erco Shimano | + 01:57 | + 01:57 |
| 31 | Cristian Munoz | Nu Colombia | + 01:57 | + 01:57 |
| 32 | Diego Hoyos Juan | Team Sistecredito | + 01:57 | + 01:57 |
| 33 | Sergio Henao | Nu Colombia | + 01:59 | + 01:59 |
| 34 | Andrea Piccolo | EF Education-EasyPost | + 03:59 | + 03:59 |
| 35 | Ramon Muniz Jose | Petrolike | + 04:18 | + 04:18 |
| 36 | Yeisson Casallas | Colombia Potencia de la Vida-Strongman | + 04:18 | + 04:18 |
| 37 | Nicolas Sessler | Brazil | + 05:46 | + 05:46 |
| 38 | Edgar Cadena | Petrolike | + 07:10 | + 07:10 |
| 39 | Dario Gomez Joya Hernan | Sidi Ali-Unlock Team | + 07:10 | + 07:10 |
| 40 | Rait Ärm | Estonia | + 07:10 | + 07:10 |
| 41 | Jip Steman | Universe Cycling Team | + 07:28 | + 07:28 |
| 42 | Kenny Nijssen | Universe Cycling Team | + 07:28 | + 07:28 |
| 43 | Diego Guerrero | Team Sistecredito | + 07:31 | + 07:31 |
| 44 | Daniel Arroyave | GW Erco Shimano | + 07:50 | + 07:50 |
| 45 | Daniel Muñoz | Nu Colombia | + 07:50 | + 07:50 |
| 46 | Alexey Lutsenko | Astana Qazaqstan Team | + 08:39 | + 08:39 |
| 47 | Alexander Gil | Orgullo Paisa | + 08:56 | + 08:56 |
| 48 | Dimitri Peyskens | Bingoal WB | + 09:28 | + 09:28 |
| 49 | Davide Baldaccini | Team corratec - Vini Fantini | + 09:28 | + 09:28 |
| 50 | Michiel Lambrecht | Bingoal WB | + 10:34 | + 10:34 |
| 51 | Danny Osorio | Orgullo Paisa | + 10:35 | + 10:35 |
| 52 | Lennert Teugels | Bingoal WB | + 10:35 | + 10:35 |
| 53 | Lars Quaedvlieg | Universe Cycling Team | + 13:47 | + 13:47 |
| 54 | Miguel Florez | Team Banco Guayaquil-Bianchi | + 15:41 | + 15:41 |
| 55 | Fabio Duarte | Team Medellin | + 15:41 | + 15:41 |
| 56 | Oscar Quiroz | Nu Colombia | + 15:41 | + 15:41 |
| 57 | Freddy Avila | Colombia Potencia de la Vida-Strongman | + 15:41 | + 15:41 |
| 58 | Luis Mora | Venezuela | + 15:41 | + 15:41 |
| 59 | Luis Pinto | Venezuela | + 15:41 | + 15:41 |
| 60 | Jeison Rujano | Venezuela | + 15:41 | + 15:41 |
| 61 | Laureano Rosas | SwiftCarbon Pro Cycling Brasil | + 15:41 | + 15:41 |
| 62 | Camilo Tobar Cristian | Team Saitel | + 15:41 | + 15:41 |
| 63 | Franklin Archibold | Panamá es Cultura y Valores | + 15:41 | + 15:41 |
| 64 | Paulo Pantoja | Orgullo Paisa | + 15:41 | + 15:41 |
| 65 | Jhojan Garcia | Colombia Potencia de la Vida-Strongman | + 15:41 | + 15:41 |
| 66 | Bernardo Suaza | Petrolike | + 15:41 | + 15:41 |
| 67 | Erik Caiza | Team Saitel | + 15:41 | + 15:41 |
| 68 | Marlon Diágama | Colombia Potencia de la Vida-Strongman | + 15:41 | + 15:41 |
| 69 | Steven Haro | Ecuador | + 15:41 | + 15:41 |
| 70 | Alexis Quinteros | Team Banco Guayaquil-Bianchi | + 15:41 | + 15:41 |
| 71 | Santiago Montenegro | Ecuador | + 15:41 | + 15:41 |
| 72 | Robinson Chalapud | Team Banco Guayaquil-Bianchi | + 15:41 | + 15:41 |
| 73 | Richard Huera | Ecuador | + 15:41 | + 15:41 |
| 74 | Diego Alba Juan | Colombia | + 15:41 | + 15:41 |
| 75 | Diego Camargo | Petrolike | + 15:41 | + 15:41 |
| 76 | Andres Parra Carlos | Team Saitel | + 15:41 | + 15:41 |
| 77 | Heiner Parra | Canel's-Java | + 15:41 | + 15:41 |
| 78 | David Guavita Cesar | Colombia Potencia de la Vida-Strongman | + 15:41 | + 15:41 |
| 79 | Santiago Gomez Marin | Team Sistecredito | + 15:41 | + 15:41 |
| 80 | Diego Ochoa | Team Saitel | + 15:41 | + 15:41 |
| 81 | Franklin Revelo | Team Saitel | + 15:41 | + 15:41 |
| 82 | Giulio Masotto | Team corratec - Vini Fantini | + 15:41 | + 15:41 |
| 83 | Efren Santos | Canel's-Java | + 15:41 | + 15:41 |
| 84 | Sebastian Castano | Team Sistecredito | + 15:41 | + 15:41 |
| 85 | Julian Cardona Tabares | Team Medellin | + 15:41 | + 15:41 |
| 86 | Lorenzo Quartucci | Team corratec - Vini Fantini | + 15:41 | + 15:41 |
| 87 | Mark Stewart | Team corratec - Vini Fantini | + 15:41 | + 15:41 |
| 88 | Kevin Jimenez Tepetl | Canel's-Java | + 19:28 | + 19:28 |
| 89 | Vladimir Lopez | Sidi Ali-Unlock Team | + 20:00 | + 20:00 |
| 90 | Attilio Viviani | Team corratec - Vini Fantini | + 20:09 | + 20:09 |
| 91 | Martin Laas | Estonia | + 20:09 | + 20:09 |
| 92 | Cristian Pita | Team Banco Guayaquil-Bianchi | + 20:09 | + 20:09 |
| 93 | Davide Persico | Bingoal WB | + 20:09 | + 20:09 |
| 94 | Fernando Gaviria | Movistar Team | + 20:09 | + 20:09 |
| 95 | Markus Pajur | Estonia | + 20:09 | + 20:09 |
| 96 | Norman Vahtra | Estonia | + 20:09 | + 20:09 |
| 97 | Victor Cesar de Paula | Brazil | + 20:27 | + 20:27 |
| 98 | Cormac Mcgeough | Canel's-Java | + 20:43 | + 20:43 |
| 99 | Kamal Mahroug | Sidi Ali-Unlock Team | + 20:43 | + 20:43 |
| 100 | Ignacio Prado | Canel's-Java | + 20:47 | + 20:47 |
| 101 | Hugo Rodriguez | Colombia | + 20:47 | + 20:47 |
| 102 | Johan Colon | Orgullo Paisa | + 20:54 | + 20:54 |
| 103 | Ivan Ojeda | Colombia Potencia de la Vida-Strongman | + 20:54 | + 20:54 |
| 104 | Milan Lanhove | Bingoal WB | + 20:54 | + 20:54 |
| 105 | Pablo Alarcon | Canel's-Java | + 20:54 | + 20:54 |
| 106 | David Villareal | Ecuador | + 20:54 | + 20:54 |
| 107 | Martti Lenzius | Estonia | + 20:54 | + 20:54 |
| 108 | Nelson Soto | Petrolike | + 20:54 | + 20:54 |
| 109 | Andre Gohr | Brazil | + 20:54 | + 20:54 |
| 110 | Cristofer Jurado | Panamá es Cultura y Valores | + 20:54 | + 20:54 |
| 111 | Brayan Obando | Ecuador | + 21:25 | + 21:25 |
| 112 | Enmanuel Viloria | Venezuela | + 21:31 | + 21:31 |
| 113 | Jhonny Araujo | Venezuela | + 21:31 | + 21:31 |
| 114 | Pedro Rossi Joao | SwiftCarbon Pro Cycling Brasil | + 21:31 | + 21:31 |
| 115 | Magno Rabelo Euller | Brazil | + 21:31 | + 21:31 |
| 116 | Randish Lorenzo | Panamá es Cultura y Valores | + 21:31 | + 21:31 |
| 117 | Alejandro Pita Hidrobo | Team Saitel | + 21:31 | + 21:31 |
| 118 | Bolivar Espinosa | Panamá es Cultura y Valores | + 21:31 | + 21:31 |
| 119 | Andrey André | Brazil | + 21:31 | + 21:31 |
| 120 | Mark Cavendish | Astana Qazaqstan Team | + 21:31 | + 21:31 |
| 121 | Rick Nobel | Universe Cycling Team | + 22:31 | + 22:31 |
| 122 | Sebastian Caicedo | Team Banco Guayaquil-Bianchi | + 22:32 | + 22:32 |
| 123 | Lauro Chaman | SwiftCarbon Pro Cycling Brasil | + 24:15 | + 24:15 |
| 124 | Felipe Chan Romero Hassan | Panamá es Cultura y Valores | + 24:49 | + 24:49 |
| 125 | Alessandro Guimaraes | SwiftCarbon Pro Cycling Brasil | + 27:46 | + 27:46 |