| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Gabriele Bosisio | Tenax - Menikini | 05:08:33 | |
| 2 | Emanuele Sella | Ceramica Panaria - Navigare | + 00 | |
| 3 | Branislau Samoilau | Acqua e Sapone | + 02 | + 02 |
| 4 | Kanstantsin Sivtsov | Barloworld | + 02 | + 02 |
| 5 | Santo Anzà | Serramenti - Selle Italia | + 02 | + 02 |
| 6 | Ruslan Pidgornyy | Tenax - Menikini | + 25 | + 25 |
| 7 | Serguei Klimov | Tinkoff Credit Systems | + 02:13 | + 02:13 |
| 8 | Maurizio Varini | Ceramica Flaminia | + 03:10 | + 03:10 |
| 9 | Patxi Vila | Lampre-Fondital | + 03:14 | + 03:14 |
| 10 | Giairo Ermeti | Tenax - Menikini | + 03:24 | + 03:24 |
| 11 | Carlo Scognamiglio | Team Milram | + 03:34 | + 03:34 |
| 12 | Dario Cataldo | Liquigas | + 03:34 | + 03:34 |
| 13 | Volodymyr Zagorodniy | OTC Doors - Lauretana | + 07:31 | + 07:31 |
| 14 | Giuseppe Palumbo | Acqua e Sapone | + 07:31 | + 07:31 |
| 15 | Efrem Salvi | Team Milram | + 07:31 | + 07:31 |
| 16 | Matej Jurco | Team Milram | + 07:31 | + 07:31 |
| 17 | Alexander Efimkin | Barloworld | + 07:31 | + 07:31 |
| 18 | Alexander Serov | Tinkoff Credit Systems | + 07:31 | + 07:31 |
| 19 | Paolo Bailetti | Team LPR | + 07:31 | + 07:31 |
| 20 | Mikhail Ignatiev | Tinkoff Credit Systems | + 07:31 | + 07:31 |
| 21 | Alessandro Bertuola | Tenax - Menikini | + 07:31 | + 07:31 |
| 22 | Leonardo Moser | Serramenti - Selle Italia | + 07:31 | + 07:31 |
| 23 | Mirko Celestino | Team Milram | + 07:31 | + 07:31 |
| 24 | Stefano Cavallari | Acqua e Sapone | + 07:31 | + 07:31 |
| 25 | Alessandro Spezialetti | Liquigas | + 07:31 | + 07:31 |
| 26 | Angel Rubiano Miguel | Ceramica Panaria - Navigare | + 07:31 | + 07:31 |
| 27 | Francesco Tizza | OTC Doors - Lauretana | + 07:31 | + 07:31 |
| 28 | Filippo Pozzato | Liquigas | + 07:31 | + 07:31 |
| 29 | Andrea Noè | Liquigas | + 07:44 | + 07:44 |
| 30 | Gabriele Missaglia | Serramenti - Selle Italia | + 07:44 | + 07:44 |
| 31 | Niklas Axelsson | Serramenti - Selle Italia | + 07:44 | + 07:44 |
| 32 | Sergio Barbero | Serramenti - Selle Italia | + 07:44 | + 07:44 |
| 33 | Daniele Bennati | Lampre-Fondital | + 07:44 | + 07:44 |
| 34 | Luca Mazzanti | Ceramica Panaria - Navigare | + 07:44 | + 07:44 |
| 35 | Fortunato Baliani | Ceramica Panaria - Navigare | + 07:44 | + 07:44 |
| 36 | Giampaolo Cheula | Barloworld | + 07:44 | + 07:44 |
| 37 | Cristian Gasperoni | Ceramica Flaminia | + 07:44 | + 07:44 |
| 38 | Ivan Rovny | Tinkoff Credit Systems | + 07:44 | + 07:44 |
| 39 | Jure Golcer | Tenax - Menikini | + 07:44 | + 07:44 |
| 40 | Alessandro Bisolti | Tinkoff Credit Systems | + 14:02 | + 14:02 |
| 41 | Massimo Codol | Acqua e Sapone | + 14:02 | + 14:02 |
| 42 | Roberto Petito | Liquigas | + 14:02 | + 14:02 |
| 43 | Daniele Pietropolli | Tenax - Menikini | + 14:02 | + 14:02 |
| 44 | Antonio D'aniello | Ceramica Flaminia | + 14:02 | + 14:02 |
| 45 | Paolo Longo Borghini | Barloworld | + 14:02 | + 14:02 |
| 46 | Ilya Chernetskiy | Tinkoff Credit Systems | + 14:20 | + 14:20 |
| 47 | Anton Mindlin | Tinkoff Credit Systems | + 14:20 | + 14:20 |
| 48 | Mirco Lorenzetto | Team Milram | + 14:20 | + 14:20 |
| 49 | Pavel Brutt | Tinkoff Credit Systems | + 14:20 | + 14:20 |
| 50 | Marco Velo | Team Milram | + 14:20 | + 14:20 |
| 51 | Domenico Quagliarella | Ceramica Flaminia | + 14:20 | + 14:20 |
| 52 | Fabio Baldato | Lampre-Fondital | + 14:20 | + 14:20 |
| 53 | Sergio Ghisalberti | Team Milram | + 14:20 | + 14:20 |
| 54 | Andrea Pagoto | Ceramica Panaria - Navigare | + 14:20 | + 14:20 |
| 55 | Cristiano Fumagalli | Ceramica Flaminia | + 14:20 | + 14:20 |
| 56 | Luca Barla | Team Milram | + 18:32 | + 18:32 |
| 57 | Maurizio Girardini | Lampre-Fondital | + 18:32 | + 18:32 |
| 58 | Alberto Di Lorenzo | OTC Doors - Lauretana | + 18:32 | + 18:32 |
| 59 | Alessio Signego | OTC Doors - Lauretana | + 21:47 | + 21:47 |
| 60 | Enrico Rossi | OTC Doors - Lauretana | + 21:47 | + 21:47 |
| 61 | Alain van der Velde | Acqua e Sapone | + 21:47 | + 21:47 |
| 62 | Fabio Terrenzio | Acqua e Sapone | + 21:47 | + 21:47 |
| 63 | Ben Swift | Barloworld | + 22:21 | + 22:21 |
| 64 | Diego Genovesi | OTC Doors - Lauretana | + 22:21 | + 22:21 |