| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Andy Schleck | Team Saxo Bank | 06:34:32 | |
| 2 | Joaquim Rodríguez | Caisse d'Epargne | + 01:16 | + 01:16 |
| 3 | Davide Rebellin | Serramenti - Androni Giocattoli | + 01:24 | + 01:24 |
| 4 | Philippe Gilbert | Silence - Lotto | + 01:24 | + 01:24 |
| 5 | Sergei Ivanov | Team Katusha | + 01:24 | + 01:24 |
| 6 | Simon Gerrans | Cervelo TestTeam | + 01:24 | + 01:24 |
| 7 | Damiano Cunego | Lampre-NGC | + 01:24 | + 01:24 |
| 8 | Benoît Vaugrenard | Française des Jeux | + 01:24 | + 01:24 |
| 9 | Alexandr Kolobnev | Team Saxo Bank | + 01:24 | + 01:24 |
| 10 | Samuel Sánchez | Euskaltel-Euskadi | + 01:24 | + 01:24 |
| 11 | Ryder Hesjedal | Garmin-Slipstream | + 01:24 | + 01:24 |
| 12 | Matteo Carrara | Vacansoleil | + 01:24 | + 01:24 |
| 13 | Jérôme Pineau | Quick Step | + 01:24 | + 01:24 |
| 14 | Óscar Freire | Rabobank | + 01:24 | + 01:24 |
| 15 | Rinaldo Nocentini | AG2R La Mondiale | + 01:24 | + 01:24 |
| 16 | Cadel Evans | Silence - Lotto | + 01:24 | + 01:24 |
| 17 | Thomas Dekker | Silence - Lotto | + 01:24 | + 01:24 |
| 18 | Rémy Di Gregorio | Française des Jeux | + 01:24 | + 01:24 |
| 19 | Alejandro Valverde | Caisse d'Epargne | + 01:24 | + 01:24 |
| 20 | Vladimir Efimkin | AG2R La Mondiale | + 01:24 | + 01:24 |
| 21 | David Le Lay | Agritubel | + 01:24 | + 01:24 |
| 22 | Maxim Iglinskiy | Astana | + 01:24 | + 01:24 |
| 23 | Fränk Schleck | Team Saxo Bank | + 01:24 | + 01:24 |
| 24 | Christophe Moreau | Agritubel | + 01:24 | + 01:24 |
| 25 | Jelle Vanendert | Silence - Lotto | + 01:24 | + 01:24 |
| 26 | Nicki Sørensen | Team Saxo Bank | + 01:24 | + 01:24 |
| 27 | David Moncoutié | Cofidis | + 01:24 | + 01:24 |
| 28 | Carlos Sastre | Cervelo TestTeam | + 01:24 | + 01:24 |
| 29 | Fabian Wegmann | Team Milram | + 01:24 | + 01:24 |
| 30 | Luca Mazzanti | Team Katusha | + 01:24 | + 01:24 |
| 31 | Gorka Verdugo | Euskaltel-Euskadi | + 01:24 | + 01:24 |
| 32 | Paul Martens | Rabobank | + 01:24 | + 01:24 |
| 33 | Anker Sørensen Chris | Team Saxo Bank | + 01:24 | + 01:24 |
| 34 | Michele Scarponi | Serramenti - Androni Giocattoli | + 01:24 | + 01:24 |
| 35 | Maxime Monfort | Team Columbia - HTC | + 01:24 | + 01:24 |
| 36 | Pierrick Fedrigo | BBOX Bouygues Telecom | + 01:24 | + 01:24 |
| 37 | Karsten Kroon | Team Saxo Bank | + 01:24 | + 01:24 |
| 38 | Kanstantsin Sivtsov | Team Columbia - HTC | + 01:35 | + 01:35 |
| 39 | Vincenzo Nibali | Liquigas | + 01:35 | + 01:35 |
| 40 | Amaël Moinard | Cofidis | + 01:38 | + 01:38 |
| 41 | Carlos Barredo | Quick Step | + 01:43 | + 01:43 |
| 42 | José Serpa | Serramenti - Androni Giocattoli | + 02:09 | + 02:09 |
| 43 | Ivan Basso | Liquigas | + 02:24 | + 02:24 |
| 44 | Philip Deignan | Cervelo TestTeam | + 02:54 | + 02:54 |
| 45 | Chris Froome | Barloworld - Bianchi | + 02:54 | + 02:54 |
| 46 | Roman Kreuziger | Liquigas | + 02:54 | + 02:54 |
| 47 | Franco Pellizotti | Liquigas | + 02:54 | + 02:54 |
| 48 | Thomas Voeckler | BBOX Bouygues Telecom | + 02:54 | + 02:54 |
| 49 | Sergey Lagutin | Vacansoleil | + 02:54 | + 02:54 |
| 50 | Robert Gesink | Rabobank | + 02:54 | + 02:54 |
| 51 | Johnny Hoogerland | Vacansoleil | + 02:54 | + 02:54 |
| 52 | Jurgen van den Broeck | Silence - Lotto | + 02:54 | + 02:54 |
| 53 | Thomas Löfkvist | Team Columbia - HTC | + 02:54 | + 02:54 |
| 54 | Alexsandr Dyachenko | Astana | + 02:54 | + 02:54 |
| 55 | Christian Pfannberger | Team Katusha | + 02:54 | + 02:54 |
| 56 | Enrico Gasparotto | Lampre-NGC | + 02:57 | + 02:57 |
| 57 | Clément Lhotellerie | Vacansoleil | + 03:06 | + 03:06 |
| 58 | Christian Knees | Team Milram | + 03:19 | + 03:19 |
| 59 | Peter Velits | Team Milram | + 03:20 | + 03:20 |
| 60 | Jan Bakelants | Topsport Vlaanderen - Mercator | + 03:20 | + 03:20 |
| 61 | Iñaki Isasi | Euskaltel-Euskadi | + 03:20 | + 03:20 |
| 62 | Alexander Efimkin | AG2R La Mondiale | + 03:26 | + 03:26 |
| 63 | Leonardo Duque | Cofidis | + 03:48 | + 03:48 |
| 64 | Assan Bazayev | Cervelo TestTeam | + 05:37 | + 05:37 |
| 65 | Michael Albasini | Team Columbia - HTC | + 06:06 | + 06:06 |
| 66 | Manuele Mori | Lampre-NGC | + 06:25 | + 06:25 |
| 67 | Rubens Bertogliati | Serramenti - Androni Giocattoli | + 06:25 | + 06:25 |
| 68 | Cyril Gautier | BBOX Bouygues Telecom | + 06:25 | + 06:25 |
| 69 | John-lee Augustyn | Barloworld - Bianchi | + 06:25 | + 06:25 |
| 70 | Haimar Zubeldia | Astana | + 06:25 | + 06:25 |
| 71 | Pierre Rolland | BBOX Bouygues Telecom | + 06:25 | + 06:25 |
| 72 | Egoi Martinez | Euskaltel-Euskadi | + 06:25 | + 06:25 |
| 73 | Addy Engels | Quick Step | + 06:25 | + 06:25 |
| 74 | Tom Danielson | Garmin-Slipstream | + 08:01 | + 08:01 |
| 75 | Nicolas Vogondy | Agritubel | + 08:01 | + 08:01 |
| 76 | Paolo Tiralongo | Lampre-NGC | + 08:01 | + 08:01 |
| 77 | Jonathan Hivert | Skil - Shimano | + 09:05 | + 09:05 |
| 78 | Hubert Dupont | AG2R La Mondiale | + 10:37 | + 10:37 |
| 79 | Marcel Wyss | Cervelo TestTeam | + 11:05 | + 11:05 |
| 80 | Francis de Greef | Silence - Lotto | + 11:05 | + 11:05 |
| 81 | Christophe Kern | Cofidis | + 11:05 | + 11:05 |
| 82 | Guillaume Levarlet | Française des Jeux | + 11:05 | + 11:05 |
| 83 | Christophe Brandt | Silence - Lotto | + 11:05 | + 11:05 |
| 84 | Craig Lewis | Team Columbia - HTC | + 11:05 | + 11:05 |
| 85 | Jakob Fuglsang | Team Saxo Bank | + 11:05 | + 11:05 |
| 86 | Dries Devenyns | Quick Step | + 11:05 | + 11:05 |
| 87 | Luis Arrieta José | AG2R La Mondiale | + 11:05 | + 11:05 |
| 88 | Linus Gerdemann | Team Milram | + 11:05 | + 11:05 |
| 89 | Sylvain Calzati | Agritubel | + 11:05 | + 11:05 |
| 90 | Óscar Pereiro | Caisse d'Epargne | + 11:05 | + 11:05 |
| 91 | Rubén Pérez Moreno | Euskaltel-Euskadi | + 11:05 | + 11:05 |
| 92 | Valerio Agnoli | Liquigas | + 11:05 | + 11:05 |
| 93 | Luis Pasamontes | Caisse d'Epargne | + 11:05 | + 11:05 |
| 94 | Iñigo Landaluze | Euskaltel-Euskadi | + 11:05 | + 11:05 |
| 95 | Bram Tankink | Rabobank | + 11:05 | + 11:05 |
| 96 | Bert de Waele | Landbouwkrediet - Colnago | + 11:05 | + 11:05 |
| 97 | Nikita Eskov | Team Katusha | + 11:05 | + 11:05 |
| 98 | Pavel Brutt | Team Katusha | + 11:05 | + 11:05 |
| 99 | Daniel Martin | Garmin-Slipstream | + 11:05 | + 11:05 |
| 100 | Nicolas Roche | AG2R La Mondiale | + 11:05 | + 11:05 |
| 101 | Francesco Bellotti | Barloworld - Bianchi | + 11:05 | + 11:05 |
| 102 | Paolo Longo Borghini | Barloworld - Bianchi | + 11:05 | + 11:05 |
| 103 | Rigoberto Uran | Caisse d'Epargne | + 11:05 | + 11:05 |
| 104 | Volodymir Gustov | Cervelo TestTeam | + 11:05 | + 11:05 |
| 105 | Alexandre Botcharov | Team Katusha | + 11:05 | + 11:05 |
| 106 | Kevin Hulsmans | Quick Step | + 11:05 | + 11:05 |
| 107 | Nico Sijmens | Cofidis | + 11:05 | + 11:05 |
| 108 | Yukiya Arashiro | BBOX Bouygues Telecom | + 14:20 | + 14:20 |
| 109 | Simon Geschke | Skil - Shimano | + 14:21 | + 14:21 |
| 110 | Marco Marcato | Vacansoleil | + 14:21 | + 14:21 |
| 111 | Xavier Florencio | Cervelo TestTeam | + 15:26 | + 15:26 |
| 112 | Alberto Losada | Caisse d'Epargne | + 15:26 | + 15:26 |
| 113 | Pieter Jacobs | Silence - Lotto | + 15:26 | + 15:26 |
| 114 | Vasil Kiryienka | Caisse d'Epargne | + 15:26 | + 15:26 |
| 115 | Serguei Klimov | Team Katusha | + 16:29 | + 16:29 |
| 116 | Johannes Fröhlinger | Team Milram | + 16:29 | + 16:29 |
| 117 | Bert Scheirlinckx | Landbouwkrediet - Colnago | + 16:29 | + 16:29 |
| 118 | Jérémy Roy | Française des Jeux | + 16:29 | + 16:29 |
| 119 | Daniele Righi | Lampre-NGC | + 16:29 | + 16:29 |