| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Alexandre Vinokourov | Astana | 06:37:49 | |
| 2 | Alexandr Kolobnev | Team Katusha | + 06 | + 06 |
| 3 | Alejandro Valverde | Caisse d'Epargne | + 01:04 | + 01:04 |
| 4 | Philippe Gilbert | Omega Pharma-Lotto | + 01:04 | + 01:04 |
| 5 | Cadel Evans | BMC Racing Team | + 01:04 | + 01:04 |
| 6 | Andy Schleck | Team Saxo Bank | + 01:07 | + 01:07 |
| 7 | Igor Anton | Euskaltel-Euskadi | + 01:07 | + 01:07 |
| 8 | Chris Horner | RadioShack | + 01:07 | + 01:07 |
| 9 | Fränk Schleck | Team Saxo Bank | + 01:07 | + 01:07 |
| 10 | Alberto Contador | Astana | + 01:07 | + 01:07 |
| 11 | Thomas Voeckler | BBOX Bouygues Telecom | + 01:18 | + 01:18 |
| 12 | Simon Gerrans | Sky Professional Cycling Team | + 01:18 | + 01:18 |
| 13 | Ryder Hesjedal | Garmin-Transitions | + 01:18 | + 01:18 |
| 14 | Benoît Vaugrenard | Française des Jeux | + 01:18 | + 01:18 |
| 15 | Paul Martens | Rabobank | + 01:18 | + 01:18 |
| 16 | Robert Gesink | Rabobank | + 01:18 | + 01:18 |
| 17 | Fabian Wegmann | Team Milram | + 01:18 | + 01:18 |
| 18 | Stefano Garzelli | Acqua e Sapone | + 01:18 | + 01:18 |
| 19 | Jurgen van den Broeck | Omega Pharma-Lotto | + 01:18 | + 01:18 |
| 20 | Carlos Barredo | Quick Step | + 01:18 | + 01:18 |
| 21 | Damiano Cunego | Lampre-Farnese Vini | + 01:18 | + 01:18 |
| 22 | Nicolas Roche | AG2R La Mondiale | + 01:22 | + 01:22 |
| 23 | Jérôme Baugnies | Topsport Vlaanderen - Mercator | + 01:22 | + 01:22 |
| 24 | Steve Cummings | Sky Professional Cycling Team | + 01:22 | + 01:22 |
| 25 | Daniel Moreno | Omega Pharma-Lotto | + 01:25 | + 01:25 |
| 26 | Xavier Tondó | Cervelo TestTeam | + 01:25 | + 01:25 |
| 27 | Yury Trofimov | BBOX Bouygues Telecom | + 01:25 | + 01:25 |
| 28 | León Sánchez Luis | Caisse d'Epargne | + 01:25 | + 01:25 |
| 29 | Vincenzo Nibali | Liquigas-Doimo | + 01:29 | + 01:29 |
| 30 | Xavier Florencio | Cervelo TestTeam | + 01:33 | + 01:33 |
| 31 | Thomas Löfkvist | Sky Professional Cycling Team | + 01:33 | + 01:33 |
| 32 | Dimitri Champion | AG2R La Mondiale | + 01:33 | + 01:33 |
| 33 | Laurens ten Dam | Rabobank | + 01:45 | + 01:45 |
| 34 | Francesco Masciarelli | Acqua e Sapone | + 01:59 | + 01:59 |
| 35 | Bram Tankink | Rabobank | + 02:02 | + 02:02 |
| 36 | Linus Gerdemann | Team Milram | + 02:08 | + 02:08 |
| 37 | David Moncoutié | Cofidis | + 02:08 | + 02:08 |
| 38 | Leonardo Bertagnolli | Androni Giocattoli | + 02:08 | + 02:08 |
| 39 | Francesco Failli | Acqua e Sapone | + 02:35 | + 02:35 |
| 40 | José Oroz Juan | Euskaltel-Euskadi | + 02:59 | + 02:59 |
| 41 | Egoi Martinez | Euskaltel-Euskadi | + 02:59 | + 02:59 |
| 42 | Christophe Le Mével | Française des Jeux | + 03:00 | + 03:00 |
| 43 | Sergei Ivanov | Team Katusha | + 03:18 | + 03:18 |
| 44 | Joaquim Rodríguez | Team Katusha | + 05:07 | + 05:07 |
| 45 | Franco Pellizotti | Liquigas-Doimo | + 05:07 | + 05:07 |
| 46 | Giampaolo Caruso | Ceramica Flaminia | + 05:07 | + 05:07 |
| 47 | Denis Menchov | Rabobank | + 05:52 | + 05:52 |
| 48 | Eduard Vorganov | Team Katusha | + 05:52 | + 05:52 |
| 49 | Jérôme Pineau | Quick Step | + 05:52 | + 05:52 |
| 50 | Vladimir Miholjevic | Acqua e Sapone | + 06:08 | + 06:08 |
| 51 | Roman Kreuziger | Liquigas-Doimo | + 06:08 | + 06:08 |
| 52 | Haimar Zubeldia | RadioShack | + 06:08 | + 06:08 |
| 53 | Steve Morabito | BMC Racing Team | + 06:08 | + 06:08 |
| 54 | Bert de Waele | Landbouwkrediet | + 07:05 | + 07:05 |
| 55 | Manuele Mori | Lampre-Farnese Vini | + 07:05 | + 07:05 |
| 56 | Massimo Codol | Acqua e Sapone | + 07:05 | + 07:05 |
| 57 | Gorazd Stangelj | Astana | + 07:05 | + 07:05 |
| 58 | Jakob Fuglsang | Team Saxo Bank | + 07:05 | + 07:05 |
| 59 | Daniel Martin | Garmin-Transitions | + 07:05 | + 07:05 |
| 60 | Pieter Weening | Rabobank | + 07:05 | + 07:05 |
| 61 | Johan Coenen | Topsport Vlaanderen - Mercator | + 09:34 | + 09:34 |
| 62 | Bert Scheirlinckx | Landbouwkrediet | + 09:34 | + 09:34 |
| 63 | Christophe Moreau | Caisse d'Epargne | + 09:34 | + 09:34 |
| 64 | Kevin de Weert | Quick Step | + 09:34 | + 09:34 |
| 65 | Laurent Lefèvre | BBOX Bouygues Telecom | + 09:34 | + 09:34 |
| 66 | Christian Knees | Team Milram | + 09:34 | + 09:34 |
| 67 | John Gadret | AG2R La Mondiale | + 09:34 | + 09:34 |
| 68 | Edward King | Cervelo TestTeam | + 09:34 | + 09:34 |
| 69 | Sylvain Calzati | Sky Professional Cycling Team | + 09:34 | + 09:34 |
| 70 | Pierre Rolland | BBOX Bouygues Telecom | + 09:34 | + 09:34 |
| 71 | Iñaki Isasi | Euskaltel-Euskadi | + 09:34 | + 09:34 |
| 72 | Tony Martin | Team HTC - Columbia | + 09:34 | + 09:34 |
| 73 | Cyril Gautier | BBOX Bouygues Telecom | + 09:34 | + 09:34 |
| 74 | Bradley Wiggins | Sky Professional Cycling Team | + 09:34 | + 09:34 |
| 75 | Christian Vande Velde | Garmin-Transitions | + 09:34 | + 09:34 |
| 76 | Dario Cataldo | Quick Step | + 09:34 | + 09:34 |
| 77 | Amaël Moinard | Cofidis | + 09:34 | + 09:34 |
| 78 | Christophe Brandt | Omega Pharma-Lotto | + 09:34 | + 09:34 |
| 79 | Francesco Gavazzi | Lampre-Farnese Vini | + 09:34 | + 09:34 |
| 80 | Rubens Bertogliati | Androni Giocattoli | + 09:34 | + 09:34 |
| 81 | Kristjan Koren | Liquigas-Doimo | + 09:34 | + 09:34 |
| 82 | Luis Pasamontes | Caisse d'Epargne | + 09:34 | + 09:34 |
| 83 | Óscar Pereiro | Astana | + 09:34 | + 09:34 |
| 84 | Valerio Agnoli | Liquigas-Doimo | + 09:34 | + 09:34 |
| 85 | Nicki Sørensen | Team Saxo Bank | + 09:34 | + 09:34 |
| 86 | Sander Armee | Topsport Vlaanderen - Mercator | + 09:34 | + 09:34 |
| 87 | Ignatas Konovalovas | Cervelo TestTeam | + 10:22 | + 10:22 |
| 88 | Maxime Monfort | Team HTC - Columbia | + 12:27 | + 12:27 |
| 89 | Alessandro Spezialetti | Lampre-Farnese Vini | + 12:27 | + 12:27 |
| 90 | Geert Verheyen | Landbouwkrediet | + 12:27 | + 12:27 |
| 91 | Christophe Kern | Cofidis | + 12:27 | + 12:27 |
| 92 | Bart Dockx | Landbouwkrediet | + 12:27 | + 12:27 |
| 93 | Jens Voigt | Team Saxo Bank | + 12:27 | + 12:27 |
| 94 | Johannes Fröhlinger | Team Milram | + 12:27 | + 12:27 |
| 95 | Preben van Hecke | Topsport Vlaanderen - Mercator | + 12:27 | + 12:27 |
| 96 | Andrea Masciarelli | Acqua e Sapone | + 12:27 | + 12:27 |
| 97 | Brent Bookwalter | BMC Racing Team | + 12:27 | + 12:27 |
| 98 | Laurent Didier | Team Saxo Bank | + 12:27 | + 12:27 |
| 99 | Grischa Niermann | Rabobank | + 12:27 | + 12:27 |
| 100 | Michael Albasini | Team HTC - Columbia | + 12:27 | + 12:27 |
| 101 | Peter Stetina | Garmin-Transitions | + 12:27 | + 12:27 |
| 102 | David Loosli | Lampre-Farnese Vini | + 12:27 | + 12:27 |
| 103 | Pieter Vanspeybrouck | Topsport Vlaanderen - Mercator | + 12:27 | + 12:27 |
| 104 | Yaroslav Popovych | RadioShack | + 12:27 | + 12:27 |
| 105 | Peter Velits | Team HTC - Columbia | + 12:27 | + 12:27 |
| 106 | Andriy Grivko | Astana | + 12:27 | + 12:27 |
| 107 | Maxim Iglinskiy | Astana | + 12:27 | + 12:27 |
| 108 | Mikel Nieve | Euskaltel-Euskadi | + 12:27 | + 12:27 |
| 109 | Kjell Carlström | Sky Professional Cycling Team | + 12:27 | + 12:27 |
| 110 | Ivan Rovny | RadioShack | + 12:27 | + 12:27 |
| 111 | Serguei Klimov | Team Katusha | + 12:27 | + 12:27 |
| 112 | Pavel Brutt | Team Katusha | + 12:27 | + 12:27 |
| 113 | Sergio Paulinho | RadioShack | + 12:27 | + 12:27 |
| 114 | Mathias Frank | BMC Racing Team | + 12:27 | + 12:27 |
| 115 | Carlos Sastre | Cervelo TestTeam | + 12:27 | + 12:27 |
| 116 | Marcel Wyss | Cervelo TestTeam | + 12:27 | + 12:27 |
| 117 | Volodymir Gustov | Cervelo TestTeam | + 12:27 | + 12:27 |
| 118 | Hubert Dupont | AG2R La Mondiale | + 12:27 | + 12:27 |
| 119 | Craig Lewis | Team HTC - Columbia | + 12:27 | + 12:27 |
| 120 | Peter Wrolich | Team Milram | + 12:27 | + 12:27 |
| 121 | Pierrick Fedrigo | BBOX Bouygues Telecom | + 12:27 | + 12:27 |
| 122 | Tejay van Garderen | Team HTC - Columbia | + 12:27 | + 12:27 |
| 123 | Luca Mazzanti | Team Katusha | + 12:27 | + 12:27 |
| 124 | Leonardo Duque | Cofidis | + 12:27 | + 12:27 |
| 125 | Daniele Righi | Lampre-Farnese Vini | + 12:27 | + 12:27 |
| 126 | Philip Deignan | Cervelo TestTeam | + 12:27 | + 12:27 |
| 127 | Jean-christophe Péraud | Omega Pharma-Lotto | + 12:35 | + 12:35 |
| 128 | Thomas de Gendt | Topsport Vlaanderen - Mercator | + 16:04 | + 16:04 |
| 129 | Dirk Bellemakers | Landbouwkrediet | + 16:04 | + 16:04 |
| 130 | Imanol Erviti | Caisse d'Epargne | + 16:04 | + 16:04 |
| 131 | Davy Commeyne | Landbouwkrediet | + 16:04 | + 16:04 |
| 132 | Matthew Busche | RadioShack | + 16:04 | + 16:04 |
| 133 | Benjamin Gourgue | Landbouwkrediet | + 16:04 | + 16:04 |
| 134 | Reinier Honig | Acqua e Sapone | + 17:51 | + 17:51 |
| 135 | Kristof Vandewalle | Topsport Vlaanderen - Mercator | + 17:51 | + 17:51 |
| 136 | Matteo Bono | Lampre-Farnese Vini | + 17:51 | + 17:51 |
| 137 | Martin Velits | Team HTC - Columbia | + 17:51 | + 17:51 |
| 138 | Chris Froome | Sky Professional Cycling Team | + 17:51 | + 17:51 |
| 139 | Stijn Devolder | Quick Step | + 17:51 | + 17:51 |
| 140 | Jérémy Roy | Française des Jeux | + 17:51 | + 17:51 |