| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 2 | Chris Froome | Sky ProCycling | + 13 | + 13 |
| 3 | Bradley Wiggins | Sky ProCycling | + 01:39 | + 01:39 |
| 4 | Bauke Mollema | Rabobank | + 02:03 | + 02:03 |
| 5 | Denis Menchov | Geox-TMC | + 03:48 | + 03:48 |
| 6 | Maxime Monfort | Leopard-Trek | + 04:13 | + 04:13 |
| 7 | Vincenzo Nibali | Liquigas-Cannondale | + 04:31 | + 04:31 |
| 8 | Jurgen van den Broeck | Omega Pharma-Lotto | + 04:45 | + 04:45 |
| 9 | Daniel Moreno | Katusha Team | + 05:20 | + 05:20 |
| 10 | Mikel Nieve | Euskaltel-Euskadi | + 05:33 | + 05:33 |
| 11 | Jakob Fuglsang | Leopard-Trek | + 05:50 | + 05:50 |
| 12 | Anker Sørensen Chris | Saxo Bank - SunGard | + 07:04 | + 07:04 |
| 13 | Daniel Martin | Garmin-Cervélo | + 07:22 | + 07:22 |
| 14 | Marzio Bruseghin | Movistar Team | + 08:52 | + 08:52 |
| 15 | Sergey Lagutin | Vacansoleil - DCM Pro Cycling Team | + 08:57 | + 08:57 |
| 16 | Nicolas Roche | AG2R La Mondiale | + 10:25 | + 10:25 |
| 17 | Wout Poels | Vacansoleil - DCM Pro Cycling Team | + 11:42 | + 11:42 |
| 18 | Robert Kiserlovski | Pro Team Astana | + 13:27 | + 13:27 |
| 19 | Joaquim Rodríguez | Katusha Team | + 15:07 | + 15:07 |
| 20 | Carlos Sastre | Geox-TMC | + 20:08 | + 20:08 |
| 21 | Eros Capecchi | Liquigas-Cannondale | + 20:22 | + 20:22 |
| 22 | Janez Brajkovic | Team RadioShack | + 21:06 | + 21:06 |
| 23 | Kevin Seeldraeyers | Quick Step | + 22:25 | + 22:25 |
| 24 | Mathieu Perget | AG2R La Mondiale | + 33:26 | + 33:26 |
| 25 | Haimar Zubeldia | Team RadioShack | + 33:29 | + 33:29 |
| 26 | Guillaume Bonnafond | AG2R La Mondiale | + 36:38 | + 36:38 |
| 27 | Sylvain Chavanel | Quick Step | + 40:02 | + 40:02 |
| 28 | Thomas Rohregger | Leopard-Trek | + 40:40 | + 40:40 |
| 29 | Francis de Greef | Omega Pharma-Lotto | + 45:16 | + 45:16 |
| 30 | Amets Txurruka | Euskaltel-Euskadi | + 47:42 | + 47:42 |
| 31 | Jan Bakelants | Omega Pharma-Lotto | + 48:06 | + 48:06 |
| 32 | Tiago Machado | Team RadioShack | + 48:25 | + 48:25 |
| 33 | Igor Anton | Euskaltel-Euskadi | + 51:08 | + 51:08 |
| 34 | Fredrik Kessiakoff | Pro Team Astana | + 51:28 | + 51:28 |
| 35 | David de La Fuente | Geox-TMC | + 01:00:03 | + 01:00:03 |
| 36 | Gorka Verdugo | Euskaltel-Euskadi | + 01:00:53 | + 01:00:53 |
| 37 | David Moncoutié | Cofidis | + 01:12:35 | + 01:12:35 |
| 38 | Dominik Nerz | Liquigas-Cannondale | + 01:13:07 | + 01:13:07 |
| 39 | Fabio Duarte | Geox-TMC | + 01:13:16 | + 01:13:16 |
| 40 | Christophe Le Mével | Garmin-Cervélo | + 01:14:00 | + 01:14:00 |
| 41 | Steven Kruijswijk | Rabobank | + 01:16:19 | + 01:16:19 |
| 42 | Vladimir Karpets | Katusha Team | + 01:20:57 | + 01:20:57 |
| 43 | Eduard Vorganov | Katusha Team | + 01:21:57 | + 01:21:57 |
| 44 | Pablo Lastras | Movistar Team | + 01:31:16 | + 01:31:16 |
| 45 | Carlos Barredo | Rabobank | + 01:31:34 | + 01:31:34 |
| 46 | Alberto Losada | Katusha Team | + 01:33:08 | + 01:33:08 |
| 47 | David Blanco | Geox-TMC | + 01:41:37 | + 01:41:37 |
| 48 | Santo Anzà | Vacansoleil - DCM Pro Cycling Team | + 01:45:51 | + 01:45:51 |
| 49 | Johannes Fröhlinger | Skil - Shimano | + 01:47:24 | + 01:47:24 |
| 50 | José Oroz Juan | Euskaltel-Euskadi | + 01:47:47 | + 01:47:47 |
| 51 | David Le Lay | AG2R La Mondiale | + 01:48:15 | + 01:48:15 |
| 52 | Thomas Löfkvist | Sky ProCycling | + 01:48:47 | + 01:48:47 |
| 53 | León Sánchez Luis | Rabobank | + 01:50:11 | + 01:50:11 |
| 54 | Przemyslaw Niemiec | Lampre-ISD | + 01:50:36 | + 01:50:36 |
| 55 | Egoi Martinez | Euskaltel-Euskadi | + 01:52:12 | + 01:52:12 |
| 56 | Davide Malacarne | Quick Step | + 01:53:44 | + 01:53:44 |
| 57 | Josep Jufré | Pro Team Astana | + 01:59:10 | + 01:59:10 |
| 58 | Francesco Gavazzi | Lampre-ISD | + 01:59:39 | + 01:59:39 |
| 59 | Andrey Mizurov | Pro Team Astana | + 02:03:48 | + 02:03:48 |
| 60 | Cyril Dessel | AG2R La Mondiale | + 02:04:38 | + 02:04:38 |
| 61 | Marco Marzano | Lampre-ISD | + 01:04:58 | + 01:04:58 |
| 62 | Olivier Kaisen | Omega Pharma-Lotto | + 02:08:52 | + 02:08:52 |
| 63 | Angel Mate Luis | Cofidis | + 02:09:06 | + 02:09:06 |
| 64 | Tanel Kangert | Pro Team Astana | + 02:10:40 | + 02:10:40 |
| 65 | Evgeni Petrov | Pro Team Astana | + 02:13:50 | + 02:13:50 |
| 66 | Manuel Garate Juan | Rabobank | + 02:14:48 | + 02:14:48 |
| 67 | Joan Horrach | Katusha Team | + 02:16:19 | + 02:16:19 |
| 68 | Koen de Kort | Skil - Shimano | + 02:18:40 | + 02:18:40 |
| 69 | Xabier Zandio | Sky ProCycling | + 02:18:45 | + 02:18:45 |
| 70 | Johan Vansummeren | Garmin-Cervélo | + 02:18:45 | + 02:18:45 |
| 71 | Iñaki Isasi | Euskaltel-Euskadi | + 02:22:10 | + 02:22:10 |
| 72 | Antonio Piedra | Andalucia Caja Granada | + 02:23:26 | + 02:23:26 |
| 73 | Nico Sijmens | Cofidis | + 02:24:00 | + 02:24:00 |
| 74 | Damiano Caruso | Liquigas-Cannondale | + 02:25:50 | + 02:25:50 |
| 75 | Tom-jelte Slagter | Rabobank | + 02:27:27 | + 02:27:27 |
| 76 | Matteo Montaguti | AG2R La Mondiale | + 02:29:14 | + 02:29:14 |
| 77 | Sebastian Lang | Omega Pharma-Lotto | + 02:33:01 | + 02:33:01 |
| 78 | Enrico Gasparotto | Pro Team Astana | + 02:35:11 | + 02:35:11 |
| 79 | Andrew Talansky | Garmin-Cervélo | + 02:35:17 | + 02:35:17 |
| 80 | Morris Possoni | Sky ProCycling | + 02:36:28 | + 02:36:28 |
| 81 | Volodymir Gustov | Saxo Bank - SunGard | + 02:39:11 | + 02:39:11 |
| 82 | Marc de Maar | Quick Step | + 02:40:57 | + 02:40:57 |
| 83 | Greg van Avermaet | BMC Racing Team | + 02:44:59 | + 02:44:59 |
| 84 | Lloyd Mondory | AG2R La Mondiale | + 02:46:03 | + 02:46:03 |
| 85 | Sergio Paulinho | Team RadioShack | + 02:46:42 | + 02:46:42 |
| 86 | Benat Intxausti | Movistar Team | + 02:46:45 | + 02:46:45 |
| 87 | Vicente Reynès | Omega Pharma-Lotto | + 02:47:40 | + 02:47:40 |
| 88 | Mauro Santambrogio | BMC Racing Team | + 02:48:01 | + 02:48:01 |
| 89 | Andrey Kashechkin | Pro Team Astana | + 02:54:57 | + 02:54:57 |
| 90 | David Bernabeu | Andalucia Caja Granada | + 02:57:45 | + 02:57:45 |
| 91 | Kevin de Weert | Quick Step | + 02:58:47 | + 02:58:47 |
| 92 | Gert Dockx | Omega Pharma-Lotto | + 02:59:00 | + 02:59:00 |
| 93 | Stuart O'grady | Leopard-Trek | + 03:00:13 | + 03:00:13 |
| 94 | Mathias Frank | BMC Racing Team | + 03:00:40 | + 03:00:40 |
| 95 | Dimitri Champion | AG2R La Mondiale | + 03:01:28 | + 03:01:28 |
| 96 | Markel Irizar | Team RadioShack | + 03:01:37 | + 03:01:37 |
| 97 | Jorge Azanza | Euskaltel-Euskadi | + 03:03:45 | + 03:03:45 |
| 98 | Luis Roldán José | Andalucia Caja Granada | + 03:04:47 | + 03:04:47 |
| 99 | Jesus Rosendo Prado | Andalucia Caja Granada | + 03:05:09 | + 03:05:09 |
| 100 | Alessandro Petacchi | Lampre-ISD | + 03:07:01 | + 03:07:01 |
| 101 | Ruslan Pidgornyy | Vacansoleil - DCM Pro Cycling Team | + 03:07:41 | + 03:07:41 |
| 102 | Alexsandr Dyachenko | Pro Team Astana | + 03:08:09 | + 03:08:09 |
| 103 | Heinrich Haussler | Garmin-Cervélo | + 03:09:09 | + 03:09:09 |
| 104 | Kristof Vandewalle | Quick Step | + 03:10:18 | + 03:10:18 |
| 105 | Valerio Agnoli | Liquigas-Cannondale | + 03:12:43 | + 03:12:43 |
| 106 | Aitor Perez Arrieta | Lampre-ISD | + 03:15:08 | + 03:15:08 |
| 107 | Manuele Mori | Lampre-ISD | + 03:15:13 | + 03:15:13 |
| 108 | Pim Ligthart | Vacansoleil - DCM Pro Cycling Team | + 03:17:01 | + 03:17:01 |
| 109 | Francesco Bellotti | Liquigas-Cannondale | + 03:17:09 | + 03:17:09 |
| 110 | Geoffroy Lequatre | Team RadioShack | + 03:20:19 | + 03:20:19 |
| 111 | Martin Kohler | BMC Racing Team | + 03:24:38 | + 03:24:38 |
| 112 | Daniele Bennati | Leopard-Trek | + 03:25:02 | + 03:25:02 |
| 113 | Matthew Busche | Team RadioShack | + 03:25:48 | + 03:25:48 |
| 114 | Adrian Palomares | Andalucia Caja Granada | + 03:25:54 | + 03:25:54 |
| 115 | Simon Geschke | Skil - Shimano | + 03:28:05 | + 03:28:05 |
| 116 | Yoann Bagot | Cofidis | + 03:28:44 | + 03:28:44 |
| 117 | Ivan Santaromita | BMC Racing Team | + 03:32:20 | + 03:32:20 |
| 118 | Nelson Oliveira | Team RadioShack | + 03:32:44 | + 03:32:44 |
| 119 | Paul Martens | Rabobank | + 03:32:57 | + 03:32:57 |
| 120 | Nicki Sørensen | Saxo Bank - SunGard | + 03:33:58 | + 03:33:58 |
| 121 | Peter Sagan | Liquigas-Cannondale | + 03:37:24 | + 03:37:24 |
| 122 | Dmitriy Kozontchuk | Geox-TMC | + 03:38:45 | + 03:38:45 |
| 123 | Michael Albasini | Team HTC - Highroad | + 03:39:23 | + 03:39:23 |
| 124 | Yury Trofimov | Katusha Team | + 03:40:05 | + 03:40:05 |
| 125 | Martin Velits | Team HTC - Highroad | + 03:42:38 | + 03:42:38 |
| 126 | Imanol Erviti | Movistar Team | + 03:43:59 | + 03:43:59 |
| 127 | Jurgen van de Walle | Omega Pharma-Lotto | + 03:47:05 | + 03:47:05 |
| 128 | Ian Stannard | Sky ProCycling | + 03:49:52 | + 03:49:52 |
| 129 | Adam Hansen | Omega Pharma-Lotto | + 03:50:18 | + 03:50:18 |
| 130 | Alberto Benitez Jose | Andalucia Caja Granada | + 03:51:37 | + 03:51:37 |
| 131 | Manuel Quinziato | BMC Racing Team | + 03:51:52 | + 03:51:52 |
| 132 | Dario Cataldo | Quick Step | + 03:52:47 | + 03:52:47 |
| 133 | Steve Houanard | AG2R La Mondiale | + 03:53:39 | + 03:53:39 |
| 134 | Vicente Toribio Jose | Andalucia Caja Granada | + 03:55:35 | + 03:55:35 |
| 135 | Luca Paolini | Katusha Team | + 03:58:33 | + 03:58:33 |
| 136 | Bert Grabsch | Team HTC - Highroad | + 03:59:03 | + 03:59:03 |
| 137 | Julien Fouchard | Cofidis | + 04:08:07 | + 04:08:07 |
| 138 | Alan Marangoni | Liquigas-Cannondale | + 04:13:04 | + 04:13:04 |
| 139 | Aaen Jørgensen Jonas | Saxo Bank - SunGard | + 04:13:04 | + 04:13:04 |
| 140 | Sep Vanmarcke | Garmin-Cervélo | + 04:15:06 | + 04:15:06 |
| 141 | Daniele Righi | Lampre-ISD | + 04:15:54 | + 04:15:54 |
| 142 | Davide Vigano | Leopard-Trek | + 04:18:11 | + 04:18:11 |
| 143 | Aleksandr Kuschynski | Katusha Team | + 04:18:29 | + 04:18:29 |
| 144 | John Degenkolb | Team HTC - Highroad | + 04:19:56 | + 04:19:56 |
| 145 | Dario Cioni | Sky ProCycling | + 04:20:20 | + 04:20:20 |
| 146 | Matteo Carrara | Vacansoleil - DCM Pro Cycling Team | + 04:20:56 | + 04:20:56 |
| 147 | Mauro Da Dalto | Liquigas-Cannondale | + 04:22:32 | + 04:22:32 |
| 148 | Pierre Cazaux | Euskaltel-Euskadi | + 04:28:14 | + 04:28:14 |
| 149 | Jaroslaw Marycz | Saxo Bank - SunGard | + 04:29:18 | + 04:29:18 |
| 150 | Yukihiro Doi | Skil - Shimano | + 04:30:47 | + 04:30:47 |
| 151 | Nikolas Maes | Quick Step | + 04:36:21 | + 04:36:21 |
| 152 | Leigh Howard | Team HTC - Highroad | + 04:38:26 | + 04:38:26 |
| 153 | Martijn Keizer | Vacansoleil - DCM Pro Cycling Team | + 04:38:27 | + 04:38:27 |
| 154 | Stijn Devolder | Vacansoleil - DCM Pro Cycling Team | + 04:39:00 | + 04:39:00 |
| 155 | Matthias Brändle | Geox-TMC | + 04:44:09 | + 04:44:09 |
| 156 | Roy Curvers | Skil - Shimano | + 04:47:39 | + 04:47:39 |
| 157 | José Haedo Juan | Saxo Bank - SunGard | + 04:50:26 | + 04:50:26 |
| 158 | Andreas Klier | Garmin-Cervélo | + 04:51:15 | + 04:51:15 |
| 159 | Alexandre Geniez | Skil - Shimano | + 04:53:16 | + 04:53:16 |
| 160 | Mads Christensen | Saxo Bank - SunGard | + 04:56:20 | + 04:56:20 |
| 161 | Nick Nuyens | Saxo Bank - SunGard | + 04:58:38 | + 04:58:38 |
| 162 | Robert Wagner | Leopard-Trek | + 05:00:07 | + 05:00:07 |
| 163 | Chris Sutton | Sky ProCycling | + 05:00:46 | + 05:00:46 |
| 164 | Albert Timmer | Skil - Shimano | + 05:07:12 | + 05:07:12 |
| 165 | Antonio Cabello Baena | Andalucia Caja Granada | + 05:11:29 | + 05:11:29 |
| 166 | Aleksejs Saramotins | Cofidis | + 05:18:22 | + 05:18:22 |
| 167 | Tom Veelers | Skil - Shimano | + 05:24:52 | + 05:24:52 |