| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | José Cobo Juan | Geox-TMC | 04:01:56 | |
| 2 | Wout Poels | Vacansoleil - DCM Pro Cycling Team | + 48 | + 48 |
| 3 | Denis Menchov | Geox-TMC | + 48 | + 48 |
| 4 | Chris Froome | Sky ProCycling | + 48 | + 48 |
| 5 | Bradley Wiggins | Sky ProCycling | + 01:21 | + 01:21 |
| 6 | Igor Anton | Euskaltel-Euskadi | + 01:21 | + 01:21 |
| 7 | Joaquim Rodríguez | Katusha Team | + 01:35 | + 01:35 |
| 8 | Maxime Monfort | Leopard-Trek | + 01:35 | + 01:35 |
| 9 | Bauke Mollema | Rabobank | + 01:35 | + 01:35 |
| 10 | Sergey Lagutin | Vacansoleil - DCM Pro Cycling Team | + 01:35 | + 01:35 |
| 11 | Daniel Martin | Garmin-Cervélo | + 01:41 | + 01:41 |
| 12 | Fabio Duarte | Geox-TMC | + 01:52 | + 01:52 |
| 13 | Mikel Nieve | Euskaltel-Euskadi | + 02:02 | + 02:02 |
| 14 | Jurgen van den Broeck | Omega Pharma-Lotto | + 02:17 | + 02:17 |
| 15 | Vincenzo Nibali | Liquigas-Cannondale | + 02:37 | + 02:37 |
| 16 | Jakob Fuglsang | Leopard-Trek | + 02:43 | + 02:43 |
| 17 | Daniel Moreno | Katusha Team | + 02:43 | + 02:43 |
| 18 | Oliver Zaugg | Leopard-Trek | + 02:43 | + 02:43 |
| 19 | Sergio Pardilla | Movistar Team | + 03:01 | + 03:01 |
| 20 | Amets Txurruka | Euskaltel-Euskadi | + 03:22 | + 03:22 |
| 21 | Anker Sørensen Chris | Saxo Bank - SunGard | + 03:32 | + 03:32 |
| 22 | Carlos Sastre | Geox-TMC | + 03:52 | + 03:52 |
| 23 | Benat Intxausti | Movistar Team | + 04:31 | + 04:31 |
| 24 | Christophe Le Mével | Garmin-Cervélo | + 04:54 | + 04:54 |
| 25 | Kevin Seeldraeyers | Quick Step | + 04:54 | + 04:54 |
| 26 | Marzio Bruseghin | Movistar Team | + 05:08 | + 05:08 |
| 27 | Tiago Machado | Team RadioShack | + 05:27 | + 05:27 |
| 28 | Josep Jufré | Pro Team Astana | + 05:38 | + 05:38 |
| 29 | Robert Kiserlovski | Pro Team Astana | + 05:38 | + 05:38 |
| 30 | David de La Fuente | Geox-TMC | + 05:56 | + 05:56 |
| 31 | Francis de Greef | Omega Pharma-Lotto | + 06:05 | + 06:05 |
| 32 | Jan Bakelants | Omega Pharma-Lotto | + 06:12 | + 06:12 |
| 33 | Nicolas Roche | AG2R La Mondiale | + 06:19 | + 06:19 |
| 34 | Alberto Losada | Katusha Team | + 06:30 | + 06:30 |
| 35 | Carlos Barredo | Rabobank | + 06:48 | + 06:48 |
| 36 | Gorka Verdugo | Euskaltel-Euskadi | + 06:51 | + 06:51 |
| 37 | Thomas Rohregger | Leopard-Trek | + 07:01 | + 07:01 |
| 38 | David López | Movistar Team | + 07:14 | + 07:14 |
| 39 | Eros Capecchi | Liquigas-Cannondale | + 07:34 | + 07:34 |
| 40 | Mathieu Perget | AG2R La Mondiale | + 07:44 | + 07:44 |
| 41 | Przemyslaw Niemiec | Lampre-ISD | + 08:05 | + 08:05 |
| 42 | Matteo Montaguti | AG2R La Mondiale | + 08:10 | + 08:10 |
| 43 | Manuel Garate Juan | Rabobank | + 08:28 | + 08:28 |
| 44 | Eduard Vorganov | Katusha Team | + 08:28 | + 08:28 |
| 45 | Angel Madrazo | Movistar Team | + 08:31 | + 08:31 |
| 46 | David Moncoutié | Cofidis | + 09:11 | + 09:11 |
| 47 | Steven Kruijswijk | Rabobank | + 09:14 | + 09:14 |
| 48 | Guillaume Bonnafond | AG2R La Mondiale | + 09:14 | + 09:14 |
| 49 | Cyril Dessel | AG2R La Mondiale | + 09:14 | + 09:14 |
| 50 | Haimar Zubeldia | Team RadioShack | + 09:40 | + 09:40 |
| 51 | Janez Brajkovic | Team RadioShack | + 09:40 | + 09:40 |
| 52 | David Blanco | Geox-TMC | + 09:40 | + 09:40 |
| 53 | Steve Houanard | AG2R La Mondiale | + 10:00 | + 10:00 |
| 54 | Vicente Toribio Jose | Andalucia Caja Granada | + 10:00 | + 10:00 |
| 55 | Sylvain Chavanel | Quick Step | + 10:37 | + 10:37 |
| 56 | Egoi Martinez | Euskaltel-Euskadi | + 10:37 | + 10:37 |
| 57 | Vladimir Karpets | Katusha Team | + 10:37 | + 10:37 |
| 58 | Adrian Palomares | Andalucia Caja Granada | + 10:46 | + 10:46 |
| 59 | Nico Sijmens | Cofidis | + 11:20 | + 11:20 |
| 60 | Marc de Maar | Quick Step | + 11:27 | + 11:27 |
| 61 | Rafal Majka | Saxo Bank - SunGard | + 12:14 | + 12:14 |
| 62 | Dominik Nerz | Liquigas-Cannondale | + 12:20 | + 12:20 |
| 63 | Matthias Brändle | Geox-TMC | + 12:20 | + 12:20 |
| 64 | Pim Ligthart | Vacansoleil - DCM Pro Cycling Team | + 13:00 | + 13:00 |
| 65 | Johannes Fröhlinger | Skil - Shimano | + 13:19 | + 13:19 |
| 66 | David Le Lay | AG2R La Mondiale | + 13:51 | + 13:51 |
| 67 | Sebastian Lang | Omega Pharma-Lotto | + 13:58 | + 13:58 |
| 68 | Francesco Bellotti | Liquigas-Cannondale | + 14:00 | + 14:00 |
| 69 | Markel Irizar | Team RadioShack | + 14:04 | + 14:04 |
| 70 | Xabier Zandio | Sky ProCycling | + 14:04 | + 14:04 |
| 71 | Volodymir Gustov | Saxo Bank - SunGard | + 14:33 | + 14:33 |
| 72 | Mauro Santambrogio | BMC Racing Team | + 14:33 | + 14:33 |
| 73 | Kevin de Weert | Quick Step | + 14:36 | + 14:36 |
| 74 | Manuele Mori | Lampre-ISD | + 14:36 | + 14:36 |
| 75 | Peter Sagan | Liquigas-Cannondale | + 14:36 | + 14:36 |
| 76 | Santo Anzà | Vacansoleil - DCM Pro Cycling Team | + 14:36 | + 14:36 |
| 77 | Ian Stannard | Sky ProCycling | + 14:36 | + 14:36 |
| 78 | Fabian Cancellara | Leopard-Trek | + 14:36 | + 14:36 |
| 79 | Olivier Kaisen | Omega Pharma-Lotto | + 14:43 | + 14:43 |
| 80 | Evgeni Petrov | Pro Team Astana | + 14:45 | + 14:45 |
| 81 | Marco Marzano | Lampre-ISD | + 14:45 | + 14:45 |
| 82 | Gert Dockx | Omega Pharma-Lotto | + 14:58 | + 14:58 |
| 83 | Joan Horrach | Katusha Team | + 15:16 | + 15:16 |
| 84 | Pablo Lastras | Movistar Team | + 15:16 | + 15:16 |
| 85 | Tom-jelte Slagter | Rabobank | + 15:16 | + 15:16 |
| 86 | Jorge Azanza | Euskaltel-Euskadi | + 15:17 | + 15:17 |
| 87 | Koen de Kort | Skil - Shimano | + 15:20 | + 15:20 |
| 88 | Geoffroy Lequatre | Team RadioShack | + 15:20 | + 15:20 |
| 89 | Thomas Löfkvist | Sky ProCycling | + 15:29 | + 15:29 |
| 90 | Morris Possoni | Sky ProCycling | + 15:29 | + 15:29 |
| 91 | Kristof Vandewalle | Quick Step | + 15:37 | + 15:37 |
| 92 | Angel Mate Luis | Cofidis | + 15:41 | + 15:41 |
| 93 | Simon Geschke | Skil - Shimano | + 15:41 | + 15:41 |
| 94 | León Sánchez Luis | Rabobank | + 15:43 | + 15:43 |
| 95 | Jurgen van de Walle | Omega Pharma-Lotto | + 15:43 | + 15:43 |
| 96 | Martin Kohler | BMC Racing Team | + 15:43 | + 15:43 |
| 97 | Pierre Cazaux | Euskaltel-Euskadi | + 15:57 | + 15:57 |
| 98 | Imanol Erviti | Movistar Team | + 16:17 | + 16:17 |
| 99 | Michael Albasini | Team HTC - Highroad | + 16:17 | + 16:17 |
| 100 | Tony Martin | Team HTC - Highroad | + 16:27 | + 16:27 |
| 101 | Mathias Frank | BMC Racing Team | + 16:27 | + 16:27 |
| 102 | Francesco Gavazzi | Lampre-ISD | + 17:19 | + 17:19 |
| 103 | Martin Velits | Team HTC - Highroad | + 17:19 | + 17:19 |
| 104 | Vicente Reynès | Omega Pharma-Lotto | + 17:19 | + 17:19 |
| 105 | Andrew Talansky | Garmin-Cervélo | + 17:41 | + 17:41 |
| 106 | Dario Cioni | Sky ProCycling | + 17:48 | + 17:48 |
| 107 | Rein Taaramäe | Cofidis | + 18:04 | + 18:04 |
| 108 | Ignatas Konovalovas | Movistar Team | + 18:04 | + 18:04 |
| 109 | Dmitriy Kozontchuk | Geox-TMC | + 18:23 | + 18:23 |
| 110 | Alessandro Petacchi | Lampre-ISD | + 18:26 | + 18:26 |
| 111 | Alexsandr Dyachenko | Pro Team Astana | + 18:28 | + 18:28 |
| 112 | José Oroz Juan | Euskaltel-Euskadi | + 18:28 | + 18:28 |
| 113 | Iñaki Isasi | Euskaltel-Euskadi | + 18:28 | + 18:28 |
| 114 | Stijn Devolder | Vacansoleil - DCM Pro Cycling Team | + 18:47 | + 18:47 |
| 115 | Bert Grabsch | Team HTC - Highroad | + 18:47 | + 18:47 |
| 116 | Yukihiro Doi | Skil - Shimano | + 18:57 | + 18:57 |
| 117 | Yoann Bagot | Cofidis | + 19:00 | + 19:00 |
| 118 | Valerio Agnoli | Liquigas-Cannondale | + 19:10 | + 19:10 |
| 119 | Damiano Caruso | Liquigas-Cannondale | + 19:47 | + 19:47 |
| 120 | Daniele Bennati | Leopard-Trek | + 19:58 | + 19:58 |
| 121 | Matteo Carrara | Vacansoleil - DCM Pro Cycling Team | + 19:58 | + 19:58 |
| 122 | Ruslan Pidgornyy | Vacansoleil - DCM Pro Cycling Team | + 20:00 | + 20:00 |
| 123 | Lloyd Mondory | AG2R La Mondiale | + 20:05 | + 20:05 |
| 124 | Luca Paolini | Katusha Team | + 20:38 | + 20:38 |
| 125 | Ivan Santaromita | BMC Racing Team | + 20:38 | + 20:38 |
| 126 | Aleksandr Kuschynski | Katusha Team | + 20:38 | + 20:38 |
| 127 | Yury Trofimov | Katusha Team | + 20:38 | + 20:38 |
| 128 | Dario Cataldo | Quick Step | + 20:38 | + 20:38 |
| 129 | Johan Vansummeren | Garmin-Cervélo | + 20:38 | + 20:38 |
| 130 | Julien Fouchard | Cofidis | + 20:43 | + 20:43 |
| 131 | Alberto Benitez Jose | Andalucia Caja Granada | + 20:53 | + 20:53 |
| 132 | Jaroslaw Marycz | Saxo Bank - SunGard | + 21:04 | + 21:04 |
| 133 | Jesus Rosendo Prado | Andalucia Caja Granada | + 21:09 | + 21:09 |
| 134 | Davide Malacarne | Quick Step | + 21:11 | + 21:11 |
| 135 | Nicki Sørensen | Saxo Bank - SunGard | + 21:11 | + 21:11 |
| 136 | Robert Wagner | Leopard-Trek | + 21:25 | + 21:25 |
| 137 | Antonio Cabello Baena | Andalucia Caja Granada | + 21:28 | + 21:28 |
| 138 | Nelson Oliveira | Team RadioShack | + 21:30 | + 21:30 |
| 139 | Greg van Avermaet | BMC Racing Team | + 21:32 | + 21:32 |
| 140 | Tanel Kangert | Pro Team Astana | + 21:45 | + 21:45 |
| 141 | Fredrik Kessiakoff | Pro Team Astana | + 21:45 | + 21:45 |
| 142 | Andrey Mizurov | Pro Team Astana | + 21:45 | + 21:45 |
| 143 | Nick Nuyens | Saxo Bank - SunGard | + 22:05 | + 22:05 |
| 144 | Andrey Kashechkin | Pro Team Astana | + 22:05 | + 22:05 |
| 145 | Aleksejs Saramotins | Cofidis | + 22:05 | + 22:05 |
| 146 | Chris Sutton | Sky ProCycling | + 22:05 | + 22:05 |
| 147 | Heinrich Haussler | Garmin-Cervélo | + 22:05 | + 22:05 |
| 148 | Roy Curvers | Skil - Shimano | + 22:05 | + 22:05 |
| 149 | Alessandro Spezialetti | Lampre-ISD | + 22:05 | + 22:05 |
| 150 | Mauro Da Dalto | Liquigas-Cannondale | + 22:05 | + 22:05 |
| 151 | Adam Hansen | Omega Pharma-Lotto | + 22:05 | + 22:05 |
| 152 | Leigh Howard | Team HTC - Highroad | + 22:05 | + 22:05 |
| 153 | Mads Christensen | Saxo Bank - SunGard | + 22:05 | + 22:05 |
| 154 | David Bernabeu | Andalucia Caja Granada | + 22:05 | + 22:05 |
| 155 | Manuel Quinziato | BMC Racing Team | + 22:10 | + 22:10 |
| 156 | Albert Timmer | Skil - Shimano | + 22:17 | + 22:17 |
| 157 | Enrico Gasparotto | Pro Team Astana | + 22:26 | + 22:26 |
| 158 | Alexandre Geniez | Skil - Shimano | + 22:28 | + 22:28 |
| 159 | Luis Roldán José | Andalucia Caja Granada | + 22:46 | + 22:46 |
| 160 | Sep Vanmarcke | Garmin-Cervélo | + 22:46 | + 22:46 |
| 161 | Antonio Piedra | Andalucia Caja Granada | + 22:46 | + 22:46 |
| 162 | Dimitri Champion | AG2R La Mondiale | + 23:07 | + 23:07 |
| 163 | Aitor Perez Arrieta | Lampre-ISD | + 23:16 | + 23:16 |
| 164 | Daniele Righi | Lampre-ISD | + 23:20 | + 23:20 |
| 165 | Nikolas Maes | Quick Step | + 23:24 | + 23:24 |
| 166 | Tom Boonen | Quick Step | + 23:24 | + 23:24 |
| 167 | José Haedo Juan | Saxo Bank - SunGard | + 23:42 | + 23:42 |
| 168 | Stuart O'grady | Leopard-Trek | + 23:42 | + 23:42 |
| 169 | Paul Martens | Rabobank | + 23:42 | + 23:42 |
| 170 | John Degenkolb | Team HTC - Highroad | + 23:42 | + 23:42 |
| 171 | Andreas Klier | Garmin-Cervélo | + 23:42 | + 23:42 |
| 172 | Davide Vigano | Leopard-Trek | + 23:42 | + 23:42 |
| 173 | Tom Veelers | Skil - Shimano | + 23:42 | + 23:42 |
| 174 | Martijn Keizer | Vacansoleil - DCM Pro Cycling Team | + 23:42 | + 23:42 |
| 175 | Alan Marangoni | Liquigas-Cannondale | + 23:42 | + 23:42 |
| 176 | Sergio Paulinho | Team RadioShack | + 23:42 | + 23:42 |
| 177 | Matthew Busche | Team RadioShack | + 23:42 | + 23:42 |
| 178 | Aaen Jørgensen Jonas | Saxo Bank - SunGard | + 23:42 | + 23:42 |