| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Michael Albasini | Team HTC - Highroad | 04:19:39 | |
| 2 | Eros Capecchi | Liquigas-Cannondale | + 00 | |
| 3 | Daniel Moreno | Katusha Team | + 00 | |
| 4 | David de La Fuente | Geox-TMC | + 00 | |
| 5 | Nicolas Roche | AG2R La Mondiale | + 00 | |
| 6 | Oliver Zaugg | Leopard-Trek | + 00 | |
| 7 | Angel Madrazo | Movistar Team | + 00 | |
| 8 | David Blanco | Geox-TMC | + 00 | |
| 9 | Mikel Nieve | Euskaltel-Euskadi | + 00 | |
| 10 | Marc de Maar | Quick Step | + 00 | |
| 11 | David Moncoutié | Cofidis | + 00 | |
| 12 | David López | Movistar Team | + 00 | |
| 13 | Olivier Kaisen | Omega Pharma-Lotto | + 00 | |
| 14 | Kevin Seeldraeyers | Quick Step | + 00 | |
| 15 | Anker Sørensen Chris | Saxo Bank - SunGard | + 00 | |
| 16 | Alberto Losada | Katusha Team | + 00 | |
| 17 | Carlos Sastre | Geox-TMC | + 00 | |
| 18 | Gorka Verdugo | Euskaltel-Euskadi | + 00 | |
| 19 | Amets Txurruka | Euskaltel-Euskadi | + 03 | + 03 |
| 20 | Jan Bakelants | Omega Pharma-Lotto | + 00 | |
| 21 | Wout Poels | Vacansoleil - DCM Pro Cycling Team | + 01:29 | + 01:29 |
| 22 | Sergey Lagutin | Vacansoleil - DCM Pro Cycling Team | + 01:33 | + 01:33 |
| 23 | Maxime Monfort | Leopard-Trek | + 01:33 | + 01:33 |
| 24 | Koen de Kort | Skil - Shimano | + 01:33 | + 01:33 |
| 25 | Davide Malacarne | Quick Step | + 01:33 | + 01:33 |
| 26 | Bradley Wiggins | Sky ProCycling | + 01:33 | + 01:33 |
| 27 | Nico Sijmens | Cofidis | + 01:33 | + 01:33 |
| 28 | Vincenzo Nibali | Liquigas-Cannondale | + 01:33 | + 01:33 |
| 29 | Martin Kohler | BMC Racing Team | + 01:33 | + 01:33 |
| 30 | Tiago Machado | Team RadioShack | + 01:33 | + 01:33 |
| 31 | Bauke Mollema | Rabobank | + 01:33 | + 01:33 |
| 32 | Joaquim Rodríguez | Katusha Team | + 01:33 | + 01:33 |
| 33 | José Cobo Juan | Geox-TMC | + 01:33 | + 01:33 |
| 34 | Francesco Gavazzi | Lampre-ISD | + 01:33 | + 01:33 |
| 35 | Fredrik Kessiakoff | Pro Team Astana | + 01:33 | + 01:33 |
| 36 | Jakob Fuglsang | Leopard-Trek | + 01:33 | + 01:33 |
| 37 | Chris Froome | Sky ProCycling | + 01:33 | + 01:33 |
| 38 | Jurgen van den Broeck | Omega Pharma-Lotto | + 01:33 | + 01:33 |
| 39 | Tanel Kangert | Pro Team Astana | + 01:33 | + 01:33 |
| 40 | Sylvain Chavanel | Quick Step | + 01:33 | + 01:33 |
| 41 | Haimar Zubeldia | Team RadioShack | + 01:33 | + 01:33 |
| 42 | Janez Brajkovic | Team RadioShack | + 01:33 | + 01:33 |
| 43 | Marzio Bruseghin | Movistar Team | + 01:33 | + 01:33 |
| 44 | Przemyslaw Niemiec | Lampre-ISD | + 01:33 | + 01:33 |
| 45 | Mathieu Perget | AG2R La Mondiale | + 01:33 | + 01:33 |
| 46 | Pablo Lastras | Movistar Team | + 01:33 | + 01:33 |
| 47 | Rafal Majka | Saxo Bank - SunGard | + 01:33 | + 01:33 |
| 48 | Sergio Pardilla | Movistar Team | + 01:33 | + 01:33 |
| 49 | Daniel Martin | Garmin-Cervélo | + 01:33 | + 01:33 |
| 50 | Denis Menchov | Geox-TMC | + 01:33 | + 01:33 |
| 51 | Robert Kiserlovski | Pro Team Astana | + 01:33 | + 01:33 |
| 52 | Manuel Garate Juan | Rabobank | + 01:33 | + 01:33 |
| 53 | Guillaume Bonnafond | AG2R La Mondiale | + 01:33 | + 01:33 |
| 54 | Cyril Dessel | AG2R La Mondiale | + 01:33 | + 01:33 |
| 55 | Antonio Piedra | Andalucia Caja Granada | + 01:33 | + 01:33 |
| 56 | Christophe Le Mével | Garmin-Cervélo | + 01:33 | + 01:33 |
| 57 | Kevin de Weert | Quick Step | + 01:33 | + 01:33 |
| 58 | David Le Lay | AG2R La Mondiale | + 01:33 | + 01:33 |
| 59 | Thomas Löfkvist | Sky ProCycling | + 01:33 | + 01:33 |
| 60 | Francis de Greef | Omega Pharma-Lotto | + 01:33 | + 01:33 |
| 61 | Carlos Barredo | Rabobank | + 01:33 | + 01:33 |
| 62 | Ignatas Konovalovas | Movistar Team | + 01:33 | + 01:33 |
| 63 | Thomas Rohregger | Leopard-Trek | + 01:33 | + 01:33 |
| 64 | Josep Jufré | Pro Team Astana | + 01:33 | + 01:33 |
| 65 | Andrey Mizurov | Pro Team Astana | + 01:48 | + 01:48 |
| 66 | Dominik Nerz | Liquigas-Cannondale | + 01:48 | + 01:48 |
| 67 | Igor Anton | Euskaltel-Euskadi | + 02:12 | + 02:12 |
| 68 | Adrian Palomares | Andalucia Caja Granada | + 02:36 | + 02:36 |
| 69 | Xabier Zandio | Sky ProCycling | + 02:42 | + 02:42 |
| 70 | Enrico Gasparotto | Pro Team Astana | + 03:01 | + 03:01 |
| 71 | Sergio Paulinho | Team RadioShack | + 03:51 | + 03:51 |
| 72 | Nelson Oliveira | Team RadioShack | + 03:53 | + 03:53 |
| 73 | Lloyd Mondory | AG2R La Mondiale | + 14:39 | + 14:39 |
| 74 | Egoi Martinez | Euskaltel-Euskadi | + 14:39 | + 14:39 |
| 75 | Andrew Talansky | Garmin-Cervélo | + 14:39 | + 14:39 |
| 76 | Johannes Fröhlinger | Skil - Shimano | + 14:39 | + 14:39 |
| 77 | Francesco Bellotti | Liquigas-Cannondale | + 14:39 | + 14:39 |
| 78 | Sep Vanmarcke | Garmin-Cervélo | + 14:39 | + 14:39 |
| 79 | Simon Geschke | Skil - Shimano | + 14:39 | + 14:39 |
| 80 | Valerio Agnoli | Liquigas-Cannondale | + 18:30 | + 18:30 |
| 81 | Nicki Sørensen | Saxo Bank - SunGard | + 18:30 | + 18:30 |
| 82 | Jorge Azanza | Euskaltel-Euskadi | + 18:30 | + 18:30 |
| 83 | Mathias Frank | BMC Racing Team | + 18:30 | + 18:30 |
| 84 | Volodymir Gustov | Saxo Bank - SunGard | + 18:30 | + 18:30 |
| 85 | Matthew Busche | Team RadioShack | + 18:30 | + 18:30 |
| 86 | Yury Trofimov | Katusha Team | + 18:30 | + 18:30 |
| 87 | Alberto Benitez Jose | Andalucia Caja Granada | + 18:30 | + 18:30 |
| 88 | Iñaki Isasi | Euskaltel-Euskadi | + 18:30 | + 18:30 |
| 89 | Yoann Bagot | Cofidis | + 18:30 | + 18:30 |
| 90 | Joan Horrach | Katusha Team | + 18:30 | + 18:30 |
| 91 | Vicente Toribio Jose | Andalucia Caja Granada | + 18:30 | + 18:30 |
| 92 | Gert Dockx | Omega Pharma-Lotto | + 18:30 | + 18:30 |
| 93 | Tom-jelte Slagter | Rabobank | + 18:30 | + 18:30 |
| 94 | José Oroz Juan | Euskaltel-Euskadi | + 18:30 | + 18:30 |
| 95 | Alessandro Spezialetti | Lampre-ISD | + 18:30 | + 18:30 |
| 96 | Daniele Righi | Lampre-ISD | + 18:30 | + 18:30 |
| 97 | Rein Taaramäe | Cofidis | + 18:30 | + 18:30 |
| 98 | Geoffroy Lequatre | Team RadioShack | + 18:30 | + 18:30 |
| 99 | Dmitriy Kozontchuk | Geox-TMC | + 18:30 | + 18:30 |
| 100 | Morris Possoni | Sky ProCycling | + 18:30 | + 18:30 |
| 101 | Steven Kruijswijk | Rabobank | + 18:30 | + 18:30 |
| 102 | Vladimir Karpets | Katusha Team | + 18:30 | + 18:30 |
| 103 | Dimitri Champion | AG2R La Mondiale | + 18:30 | + 18:30 |
| 104 | Luca Paolini | Katusha Team | + 18:30 | + 18:30 |
| 105 | Matteo Montaguti | AG2R La Mondiale | + 18:30 | + 18:30 |
| 106 | Marco Marzano | Lampre-ISD | + 21:00 | + 21:00 |
| 107 | Jesus Rosendo Prado | Andalucia Caja Granada | + 21:00 | + 21:00 |
| 108 | Martijn Keizer | Vacansoleil - DCM Pro Cycling Team | + 21:00 | + 21:00 |
| 109 | Vicente Reynès | Omega Pharma-Lotto | + 21:00 | + 21:00 |
| 110 | Julien Fouchard | Cofidis | + 21:00 | + 21:00 |
| 111 | Imanol Erviti | Movistar Team | + 21:00 | + 21:00 |
| 112 | Angel Mate Luis | Cofidis | + 21:00 | + 21:00 |
| 113 | Jaroslaw Marycz | Saxo Bank - SunGard | + 21:00 | + 21:00 |
| 114 | Mads Christensen | Saxo Bank - SunGard | + 21:00 | + 21:00 |
| 115 | Dario Cataldo | Quick Step | + 21:00 | + 21:00 |
| 116 | Karsten Kroon | BMC Racing Team | + 21:00 | + 21:00 |
| 117 | Stijn Devolder | Vacansoleil - DCM Pro Cycling Team | + 21:00 | + 21:00 |
| 118 | Markel Irizar | Team RadioShack | + 21:00 | + 21:00 |
| 119 | Martin Velits | Team HTC - Highroad | + 21:00 | + 21:00 |
| 120 | Heinrich Haussler | Garmin-Cervélo | + 21:00 | + 21:00 |
| 121 | Matteo Carrara | Vacansoleil - DCM Pro Cycling Team | + 21:00 | + 21:00 |
| 122 | Ian Stannard | Sky ProCycling | + 21:00 | + 21:00 |
| 123 | Pim Ligthart | Vacansoleil - DCM Pro Cycling Team | + 21:00 | + 21:00 |
| 124 | Alessandro Petacchi | Lampre-ISD | + 21:00 | + 21:00 |
| 125 | Aleksandr Kuschynski | Katusha Team | + 21:00 | + 21:00 |
| 126 | Yukihiro Doi | Skil - Shimano | + 21:00 | + 21:00 |
| 127 | Benat Intxausti | Movistar Team | + 21:00 | + 21:00 |
| 128 | David Bernabeu | Andalucia Caja Granada | + 21:00 | + 21:00 |
| 129 | Matthias Brändle | Geox-TMC | + 21:00 | + 21:00 |
| 130 | Aitor Perez Arrieta | Lampre-ISD | + 21:00 | + 21:00 |
| 131 | Fabio Duarte | Geox-TMC | + 21:00 | + 21:00 |
| 132 | Mauro Santambrogio | BMC Racing Team | + 21:00 | + 21:00 |
| 133 | Michele Scarponi | Lampre-ISD | + 21:00 | + 21:00 |
| 134 | Andrey Kashechkin | Pro Team Astana | + 21:00 | + 21:00 |
| 135 | Manuel Quinziato | BMC Racing Team | + 21:00 | + 21:00 |
| 136 | Damiano Caruso | Liquigas-Cannondale | + 21:00 | + 21:00 |
| 137 | Aaen Jørgensen Jonas | Saxo Bank - SunGard | + 21:00 | + 21:00 |
| 138 | Eduard Vorganov | Katusha Team | + 21:00 | + 21:00 |
| 139 | Evgeni Petrov | Pro Team Astana | + 21:00 | + 21:00 |
| 140 | Alexsandr Dyachenko | Pro Team Astana | + 21:00 | + 21:00 |
| 141 | Johan Vansummeren | Garmin-Cervélo | + 21:00 | + 21:00 |
| 142 | Dario Cioni | Sky ProCycling | + 21:00 | + 21:00 |
| 143 | Steve Houanard | AG2R La Mondiale | + 21:00 | + 21:00 |
| 144 | Sebastian Lang | Omega Pharma-Lotto | + 21:00 | + 21:00 |
| 145 | León Sánchez Luis | Rabobank | + 21:00 | + 21:00 |
| 146 | Santo Anzà | Vacansoleil - DCM Pro Cycling Team | + 21:00 | + 21:00 |
| 147 | Mauro Da Dalto | Liquigas-Cannondale | + 27:50 | + 27:50 |
| 148 | Nick Nuyens | Saxo Bank - SunGard | + 27:50 | + 27:50 |
| 149 | Adam Hansen | Omega Pharma-Lotto | + 27:50 | + 27:50 |
| 150 | Kristof Vandewalle | Quick Step | + 27:50 | + 27:50 |
| 151 | Tony Martin | Team HTC - Highroad | + 27:50 | + 27:50 |
| 152 | Paul Martens | Rabobank | + 27:50 | + 27:50 |
| 153 | Chris Sutton | Sky ProCycling | + 27:50 | + 27:50 |
| 154 | Leigh Howard | Team HTC - Highroad | + 27:50 | + 27:50 |
| 155 | Daniele Bennati | Leopard-Trek | + 27:50 | + 27:50 |
| 156 | Bert Grabsch | Team HTC - Highroad | + 27:50 | + 27:50 |
| 157 | Albert Timmer | Skil - Shimano | + 27:50 | + 27:50 |
| 158 | Tom Boonen | Quick Step | + 27:50 | + 27:50 |
| 159 | Jurgen van de Walle | Omega Pharma-Lotto | + 27:50 | + 27:50 |
| 160 | José Haedo Juan | Saxo Bank - SunGard | + 27:50 | + 27:50 |
| 161 | Roy Curvers | Skil - Shimano | + 27:50 | + 27:50 |
| 162 | Alan Marangoni | Liquigas-Cannondale | + 27:50 | + 27:50 |
| 163 | Ruslan Pidgornyy | Vacansoleil - DCM Pro Cycling Team | + 27:50 | + 27:50 |
| 164 | Manuele Mori | Lampre-ISD | + 27:50 | + 27:50 |
| 165 | Greg van Avermaet | BMC Racing Team | + 27:50 | + 27:50 |
| 166 | Robert Wagner | Leopard-Trek | + 27:50 | + 27:50 |
| 167 | John Degenkolb | Team HTC - Highroad | + 27:50 | + 27:50 |
| 168 | Nikolas Maes | Quick Step | + 27:50 | + 27:50 |
| 169 | Tom Veelers | Skil - Shimano | + 27:50 | + 27:50 |
| 170 | Ivan Santaromita | BMC Racing Team | + 27:50 | + 27:50 |
| 171 | Peter Sagan | Liquigas-Cannondale | + 27:50 | + 27:50 |
| 172 | Stuart O'grady | Leopard-Trek | + 27:50 | + 27:50 |
| 173 | Andreas Klier | Garmin-Cervélo | + 27:50 | + 27:50 |
| 174 | Davide Vigano | Leopard-Trek | + 27:50 | + 27:50 |
| 175 | Antonio Cabello Baena | Andalucia Caja Granada | + 27:50 | + 27:50 |
| 176 | Luis Roldán José | Andalucia Caja Granada | + 27:50 | + 27:50 |
| 177 | Aleksejs Saramotins | Cofidis | + 27:50 | + 27:50 |
| 178 | Pierre Cazaux | Euskaltel-Euskadi | + 27:50 | + 27:50 |
| 179 | Fabian Cancellara | Leopard-Trek | + 27:50 | + 27:50 |
| 180 | Alexandre Geniez | Skil - Shimano | + 27:50 | + 27:50 |