| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Fabio Jakobsen | Soudal Quick-Step | 04:22:22 | |
| 2 | Fernando Gaviria | Movistar Team | + 00 | |
| 3 | Jon Aberasturi | Lidl-Trek | + 00 | |
| 4 | Sam Bennett | BORA-hansgrohe | + 00 | |
| 5 | Nicolas Tivani | Corratec Selle Italia | + 00 | |
| 6 | Giacomo Nizzolo | Israel-Premier Tech | + 00 | |
| 7 | Tobias Lund Andresen | Team dsm-firmenich | + 00 | |
| 8 | Elia Viviani | INEOS Grenadiers | + 00 | |
| 9 | Peter Sagan | TotalEnergies | + 00 | |
| 10 | Niklas Märkl | Team dsm-firmenich | + 00 | |
| 11 | Enrico Zanoncello | Green Project-Bardiani CSF-Faizanè | + 00 | |
| 12 | Attilio Viviani | Corratec Selle Italia | + 00 | |
| 13 | Danny van Poppel | BORA-hansgrohe | + 00 | |
| 14 | Daniel Oss | TotalEnergies | + 00 | |
| 15 | Gleb Syritsa | Astana Qazaqstan Team | + 00 | |
| 16 | Iker Bonillo | Green Project-Bardiani CSF-Faizanè | + 00 | |
| 17 | Filippo Magli | Green Project-Bardiani CSF-Faizanè | + 00 | |
| 18 | Maximiliano Richeze | Argentina | + 00 | |
| 19 | Cristian Pita | Team Banco Guayaquil | + 00 | |
| 20 | Michael Mørkøv | Soudal Quick-Step | + 00 | |
| 21 | Ángel López Miguel | Team Medellin-EPM | + 00 | |
| 22 | Miguel Salgueiro | AP Hotels & Resorts / Tavira / SC Farense | + 00 | |
| 23 | Sam Welsford | Team dsm-firmenich | + 00 | |
| 24 | Steven Haro | Team Banco Guayaquil | + 00 | |
| 25 | Remco Evenepoel | Soudal Quick-Step | + 00 | |
| 26 | Andrey Zeits | Astana Qazaqstan Team | + 00 | |
| 27 | Rick Zabel | Israel-Premier Tech | + 00 | |
| 28 | Bredio Ruiz | Panamá es Cultura y Valores | + 00 | |
| 29 | Daniel Martinez | INEOS Grenadiers | + 00 | |
| 30 | Quinn Simmons | Lidl-Trek | + 00 | |
| 31 | Egan Bernal | INEOS Grenadiers | + 00 | |
| 32 | Antwan Tolhoek | Lidl-Trek | + 00 | |
| 33 | Marco Brenner | Team dsm-firmenich | + 00 | |
| 34 | Ryan Mullen | BORA-hansgrohe | + 00 | |
| 35 | Vinicius Rangel | Movistar Team | + 00 | |
| 36 | Yves Lampaert | Soudal Quick-Step | + 00 | |
| 37 | Mathias Vacek | Lidl-Trek | + 00 | |
| 38 | Mattia Pinazzi | Italy | + 00 | |
| 39 | Alexander Konychev | Corratec Selle Italia | + 00 | |
| 40 | Iversby Hvideberg Jonas | Team dsm-firmenich | + 00 | |
| 41 | Laureano Rosas | Gremios por el Deporte Cutral Co. | + 00 | |
| 42 | Giovanni Lonardi | EOLO-Kometa Cycling Team | + 00 | |
| 43 | Harold Tejada | Astana Qazaqstan Team | + 00 | |
| 44 | Carlos Gutiérrez | Team Banco Guayaquil | + 00 | |
| 45 | Steff Cras | TotalEnergies | + 00 | |
| 46 | Albert Torres | Movistar Team | + 00 | |
| 47 | Filippo Ganna | INEOS Grenadiers | + 00 | |
| 48 | Sergio Higuita | BORA-hansgrohe | + 00 | |
| 49 | Einer Rubio | Movistar Team | + 00 | |
| 50 | Matthew Riccitello | Israel-Premier Tech | + 00 | |
| 51 | Leandro Messineo | Chimbas te Quiero | + 00 | |
| 52 | Nicolas Paredes | SEP San Juan | + 00 | |
| 53 | Lukas Dundic | Argentina | + 00 | |
| 54 | Erik Fetter | EOLO-Kometa Cycling Team | + 00 | |
| 55 | Brandon Rivera | INEOS Grenadiers | + 00 | |
| 56 | Yuriy Natarov | Astana Qazaqstan Team | + 00 | |
| 57 | Vicente Rojas | Chile | + 00 | |
| 58 | Jens Reynders | Israel-Premier Tech | + 00 | |
| 59 | Abner González | Movistar Team | + 00 | |
| 60 | Fabio Duarte | Team Medellin-EPM | + 00 | |
| 61 | Martin López | Astana Qazaqstan Team | + 00 | |
| 62 | Cristofer Jurado | Panamá es Cultura y Valores | + 00 | |
| 63 | Alan Jousseaume | TotalEnergies | + 00 | |
| 64 | Pablo Dotti Juan | SEP San Juan | + 00 | |
| 65 | Ricardo Escuela | EC Municipalidad de Pocito | + 00 | |
| 66 | Oscar Sevilla | Team Medellin-EPM | + 00 | |
| 67 | Davide Bais | EOLO-Kometa Cycling Team | + 00 | |
| 68 | Oier Lazkano | Movistar Team | + 00 | |
| 69 | Dario Cataldo | Lidl-Trek | + 00 | |
| 70 | Franco Buchanan | AV Fatima-San Juan Biker Motos-Electro 3 | + 00 | |
| 71 | Ricardo Dominguez Mauricio | AV Fatima-San Juan Biker Motos-Electro 3 | + 00 | |
| 72 | Manlio Moro | Italy | + 00 | |
| 73 | Victor Ocampo | Team Medellin-EPM | + 00 | |
| 74 | Alexis Quinteros | Team Banco Guayaquil | + 00 | |
| 75 | Emiliano Ibarra | Gremios por el Deporte Cutral Co. | + 00 | |
| 76 | Yevgeniy Gidich | Astana Qazaqstan Team | + 00 | |
| 77 | Venceslau Fernandes | AP Hotels & Resorts / Tavira / SC Farense | + 00 | |
| 78 | Maximiliano Navarrete | Gremios por el Deporte Cutral Co. | + 00 | |
| 79 | Alexander Gil | AV Fatima-San Juan Biker Motos-Electro 3 | + 00 | |
| 80 | Brayan Sanchez | Team Medellin-EPM | + 00 | |
| 81 | Delio Fernandez | AP Hotels & Resorts / Tavira / SC Farense | + 00 | |
| 82 | Eric Fagundez | Uruguay | + 00 | |
| 83 | Mateo Kalejmann | SEP San Juan | + 00 | |
| 84 | Jonas Koch | BORA-hansgrohe | + 00 | |
| 85 | Marco Frigo | Israel-Premier Tech | + 00 | |
| 86 | Daniel Diaz Rodrigo | Municipalidad de Rawson | + 00 | |
| 87 | Javier Jamaica | Team Medellin-EPM | + 00 | |
| 88 | Hugo Ruiz | SEP San Juan | + 00 | |
| 89 | Mattia Petrucci | Green Project-Bardiani CSF-Faizanè | + 00 | |
| 90 | Bolivar Espinosa | Panamá es Cultura y Valores | + 00 | |
| 91 | Cristhian Montoya | Team Banco Guayaquil | + 00 | |
| 92 | Franklin Archibold | Panamá es Cultura y Valores | + 00 | |
| 93 | Maciej Bodnar | TotalEnergies | + 39 | + 39 |
| 94 | Martin Reyes Jose | EC Municipalidad de Pocito | + 44 | + 44 |
| 95 | Manuel Lira Eguiguren | Chile | + 46 | + 46 |
| 96 | Hector Quintana | Chile | + 46 | + 46 |
| 97 | Javier Snihur Maximiliano | SEP San Juan | + 46 | + 46 |
| 98 | Manuele Tarozzi | Green Project-Bardiani CSF-Faizanè | + 46 | + 46 |
| 99 | Nicolas Traico Maximiliano | Gremios por el Deporte Cutral Co. | + 46 | + 46 |
| 100 | Alex Strah | Panamá es Cultura y Valores | + 46 | + 46 |
| 101 | Emiliano Contreras | Chimbas te Quiero | + 46 | + 46 |
| 102 | Alejandro Quilci | Gremios por el Deporte Cutral Co. | + 46 | + 46 |
| 103 | Luis Rodriguez Jose | Chile | + 46 | + 46 |
| 104 | German Lopez Franco | Argentina | + 46 | + 46 |
| 105 | Simone Bevilacqua | EOLO-Kometa Cycling Team | + 53 | + 53 |
| 106 | Stefano Gandin | Corratec Selle Italia | + 01:03 | + 01:03 |
| 107 | Diego Sevilla | EOLO-Kometa Cycling Team | + 01:06 | + 01:06 |
| 108 | Pieter Serry | Soudal Quick-Step | + 01:37 | + 01:37 |
| 109 | Florian Lipowitz | BORA-hansgrohe | + 02:37 | + 02:37 |
| 110 | Roderyck Asconeguy | Uruguay | + 01:37 | + 01:37 |
| 111 | Simone Raccani | EOLO-Kometa Cycling Team | + 01:37 | + 01:37 |
| 112 | Rafael Lourenço | AP Hotels & Resorts / Tavira / SC Farense | + 01:46 | + 01:46 |
| 113 | Tomas Contte | Argentina | + 02:01 | + 02:01 |
| 114 | Jan Hirt | Soudal Quick-Step | + 02:09 | + 02:09 |
| 115 | Kevin Vermaerke | Team dsm-firmenich | + 00 | |
| 116 | Liam Bertazzo | Italy | + 02:26 | + 02:26 |
| 117 | Fabien Grellier | TotalEnergies | + 03:20 | + 03:20 |
| 118 | Mauricio Paez | Gremios por el Deporte Cutral Co. | + 03:24 | + 03:24 |
| 119 | Ruben Ramos | EC Municipalidad de Pocito | + 03:24 | + 03:24 |
| 120 | Mauro Richeze | Chimbas te Quiero | + 03:31 | + 03:31 |
| 121 | Alessandro Santaromita | Green Project-Bardiani CSF-Faizanè | + 03:32 | + 03:32 |
| 122 | Stephen Williams | Israel-Premier Tech | + 03:32 | + 03:32 |
| 123 | Davide Boscaro | Italy | + 05:18 | + 05:18 |
| 124 | Michele Scartezzini | Italy | + 05:18 | + 05:18 |
| 125 | Francesco Lamon | Italy | + 05:18 | + 05:18 |
| 126 | Francisco Kotsakis Lagos | Chile | + 08:08 | + 08:08 |
| 127 | Marcos Mendez | Argentina | + 08:08 | + 08:08 |
| 128 | Ruben Simao | AP Hotels & Resorts / Tavira / SC Farense | + 08:08 | + 08:08 |
| 129 | Daniel Juarez | AV Fatima-San Juan Biker Motos-Electro 3 | + 12:21 | + 12:21 |
| 130 | Juan Rodriguez Ignacio | Argentina | + 12:21 | + 12:21 |
| 131 | Wladimir Martinez | AV Fatima-San Juan Biker Motos-Electro 3 | + 12:21 | + 12:21 |
| 132 | Gerardo Tivani | AV Fatima-San Juan Biker Motos-Electro 3 | + 12:21 | + 12:21 |
| 133 | Agustin Videla | Municipalidad de Rawson | + 12:21 | + 12:21 |
| 134 | Yelko Gomez | Panamá es Cultura y Valores | + 12:21 | + 12:21 |
| 135 | Nahuel Mendez Marcelo | Municipalidad de Rawson | + 12:21 | + 12:21 |
| 136 | Leandro Velardez | EC Municipalidad de Pocito | + 21:55 | + 21:55 |
| 137 | Sergio Aguirre | Municipalidad de Rawson | + 21:55 | + 21:55 |
| 138 | Leonardo Cobarrubia | SEP San Juan | + 21:55 | + 21:55 |
| 139 | Javier Melivillo Juan | EC Municipalidad de Pocito | + 32:15 | + 32:15 |
| 140 | Nixon Rosero | Team Banco Guayaquil | + 34:49 | + 34:49 |
| 141 | Fernando Mendez | Uruguay | + 34:49 | + 34:49 |
| 142 | Alejandro Duran | Chimbas te Quiero | + 34:49 | + 34:49 |
| 143 | Nicolas Cabrera | Chile | + 34:49 | + 34:49 |
| 144 | Javier Lopez Federico | EC Municipalidad de Pocito | + 34:49 | + 34:49 |
| 145 | Pedro Gordillo | Municipalidad de Rawson | + 34:49 | + 34:49 |
| 146 | Juan Caorsi | Uruguay | + 34:49 | + 34:49 |