| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Ángel López Miguel | Team Medellin-EPM | 04:07:10 | |
| 2 | Filippo Ganna | INEOS Grenadiers | + 30 | + 30 |
| 3 | Sergio Higuita | BORA-hansgrohe | + 38 | + 38 |
| 4 | Egan Bernal | INEOS Grenadiers | + 40 | + 40 |
| 5 | Einer Rubio | Movistar Team | + 40 | + 40 |
| 6 | Brandon Rivera | INEOS Grenadiers | + 51 | + 51 |
| 7 | Remco Evenepoel | Soudal Quick-Step | + 01:09 | + 01:09 |
| 8 | Nicolas Paredes | SEP San Juan | + 01:09 | + 01:09 |
| 9 | Kevin Vermaerke | Team dsm-firmenich | + 01:11 | + 01:11 |
| 10 | Quinn Simmons | Lidl-Trek | + 01:31 | + 01:31 |
| 11 | Matthew Riccitello | Israel-Premier Tech | + 01:31 | + 01:31 |
| 12 | Oier Lazkano | Movistar Team | + 01:41 | + 01:41 |
| 13 | Leandro Messineo | Chimbas te Quiero | + 01:46 | + 01:46 |
| 14 | Alessandro Santaromita | Green Project-Bardiani CSF-Faizanè | + 01:50 | + 01:50 |
| 15 | Nicolas Tivani | Corratec Selle Italia | + 01:51 | + 01:51 |
| 16 | Pablo Dotti Juan | SEP San Juan | + 02:02 | + 02:02 |
| 17 | Steff Cras | TotalEnergies | + 02:02 | + 02:02 |
| 18 | Vicente Rojas | Chile | + 02:02 | + 02:02 |
| 19 | Florian Lipowitz | BORA-hansgrohe | + 02:02 | + 02:02 |
| 20 | Abner González | Movistar Team | + 02:02 | + 02:02 |
| 21 | Harold Tejada | Astana Qazaqstan Team | + 02:06 | + 02:06 |
| 22 | Marco Frigo | Israel-Premier Tech | + 02:08 | + 02:08 |
| 23 | Daniel Martinez | INEOS Grenadiers | + 02:15 | + 02:15 |
| 24 | Jonas Koch | BORA-hansgrohe | + 02:16 | + 02:16 |
| 25 | Yves Lampaert | Soudal Quick-Step | + 02:20 | + 02:20 |
| 26 | Oscar Sevilla | Team Medellin-EPM | + 02:20 | + 02:20 |
| 27 | Manuele Tarozzi | Green Project-Bardiani CSF-Faizanè | + 02:30 | + 02:30 |
| 28 | Jan Hirt | Soudal Quick-Step | + 02:39 | + 02:39 |
| 29 | Brayan Sanchez | Team Medellin-EPM | + 02:45 | + 02:45 |
| 30 | Martin López | Astana Qazaqstan Team | + 02:55 | + 02:55 |
| 31 | Alan Jousseaume | TotalEnergies | + 03:02 | + 03:02 |
| 32 | Yuriy Natarov | Astana Qazaqstan Team | + 03:02 | + 03:02 |
| 33 | Filippo Magli | Green Project-Bardiani CSF-Faizanè | + 03:02 | + 03:02 |
| 34 | Steven Haro | Team Banco Guayaquil | + 03:16 | + 03:16 |
| 35 | Franklin Archibold | Panamá es Cultura y Valores | + 03:25 | + 03:25 |
| 36 | Antwan Tolhoek | Lidl-Trek | + 03:41 | + 03:41 |
| 37 | Diego Sevilla | EOLO-Kometa Cycling Team | + 03:47 | + 03:47 |
| 38 | Franco Buchanan | AV Fatima-San Juan Biker Motos-Electro 3 | + 03:58 | + 03:58 |
| 39 | Mathias Vacek | Lidl-Trek | + 04:16 | + 04:16 |
| 40 | Mattia Petrucci | Green Project-Bardiani CSF-Faizanè | + 04:23 | + 04:23 |
| 41 | Fabien Grellier | TotalEnergies | + 04:27 | + 04:27 |
| 42 | Rafael Lourenço | AP Hotels & Resorts / Tavira / SC Farense | + 04:36 | + 04:36 |
| 43 | Stefano Gandin | Corratec Selle Italia | + 04:49 | + 04:49 |
| 44 | Cristhian Montoya | Team Banco Guayaquil | + 04:55 | + 04:55 |
| 45 | Pieter Serry | Soudal Quick-Step | + 04:55 | + 04:55 |
| 46 | Eric Fagundez | Uruguay | + 05:08 | + 05:08 |
| 47 | Delio Fernandez | AP Hotels & Resorts / Tavira / SC Farense | + 05:08 | + 05:08 |
| 48 | Andrey Zeits | Astana Qazaqstan Team | + 05:08 | + 05:08 |
| 49 | Nixon Rosero | Team Banco Guayaquil | + 07:12 | + 07:12 |
| 50 | Venceslau Fernandes | AP Hotels & Resorts / Tavira / SC Farense | + 07:12 | + 07:12 |
| 51 | Javier Jamaica | Team Medellin-EPM | + 07:12 | + 07:12 |
| 52 | Alexander Gil | AV Fatima-San Juan Biker Motos-Electro 3 | + 07:12 | + 07:12 |
| 53 | Erik Fetter | EOLO-Kometa Cycling Team | + 07:14 | + 07:14 |
| 54 | Alexander Konychev | Corratec Selle Italia | + 07:52 | + 07:52 |
| 55 | Hector Quintana | Chile | + 07:58 | + 07:58 |
| 56 | Laureano Rosas | Gremios por el Deporte Cutral Co. | + 08:01 | + 08:01 |
| 57 | Nicolas Traico Maximiliano | Gremios por el Deporte Cutral Co. | + 08:01 | + 08:01 |
| 58 | Daniel Diaz Rodrigo | Municipalidad de Rawson | + 08:07 | + 08:07 |
| 59 | Leonardo Cobarrubia | SEP San Juan | + 08:19 | + 08:19 |
| 60 | Marco Brenner | Team dsm-firmenich | + 08:26 | + 08:26 |
| 61 | Niklas Märkl | Team dsm-firmenich | + 08:26 | + 08:26 |
| 62 | Alejandro Duran | Chimbas te Quiero | + 08:39 | + 08:39 |
| 63 | Iversby Hvideberg Jonas | Team dsm-firmenich | + 09:18 | + 09:18 |
| 64 | Martin Reyes Jose | EC Municipalidad de Pocito | + 09:26 | + 09:26 |
| 65 | Bolivar Espinosa | Panamá es Cultura y Valores | + 09:44 | + 09:44 |
| 66 | Ricardo Escuela | EC Municipalidad de Pocito | + 10:17 | + 10:17 |
| 67 | Emiliano Ibarra | Gremios por el Deporte Cutral Co. | + 10:17 | + 10:17 |
| 68 | Ruben Simao | AP Hotels & Resorts / Tavira / SC Farense | + 10:37 | + 10:37 |
| 69 | Miguel Salgueiro | AP Hotels & Resorts / Tavira / SC Farense | + 10:37 | + 10:37 |
| 70 | Daniel Oss | TotalEnergies | + 10:37 | + 10:37 |
| 71 | Carlos Gutiérrez | Team Banco Guayaquil | + 10:37 | + 10:37 |
| 72 | Danny van Poppel | BORA-hansgrohe | + 10:37 | + 10:37 |
| 73 | Tobias Lund Andresen | Team dsm-firmenich | + 10:37 | + 10:37 |
| 74 | Vinicius Rangel | Movistar Team | + 13:35 | + 13:35 |
| 75 | Albert Torres | Movistar Team | + 13:35 | + 13:35 |
| 76 | Alex Strah | Panamá es Cultura y Valores | + 13:35 | + 13:35 |
| 77 | Dario Cataldo | Lidl-Trek | + 13:35 | + 13:35 |
| 78 | Nahuel Mendez Marcelo | Municipalidad de Rawson | + 15:26 | + 15:26 |
| 79 | Simone Raccani | EOLO-Kometa Cycling Team | + 16:24 | + 16:24 |
| 80 | Pedro Gordillo | Municipalidad de Rawson | + 16:43 | + 16:43 |
| 81 | Javier Snihur Maximiliano | SEP San Juan | + 17:21 | + 17:21 |
| 82 | Enrico Zanoncello | Green Project-Bardiani CSF-Faizanè | + 17:21 | + 17:21 |
| 83 | Giacomo Nizzolo | Israel-Premier Tech | + 17:21 | + 17:21 |
| 84 | Jens Reynders | Israel-Premier Tech | + 17:21 | + 17:21 |
| 85 | Iker Bonillo | Green Project-Bardiani CSF-Faizanè | + 17:21 | + 17:21 |
| 86 | Rick Zabel | Israel-Premier Tech | + 17:21 | + 17:21 |
| 87 | Attilio Viviani | Corratec Selle Italia | + 17:21 | + 17:21 |
| 88 | Sergio Aguirre | Municipalidad de Rawson | + 17:21 | + 17:21 |
| 89 | Cristofer Jurado | Panamá es Cultura y Valores | + 17:21 | + 17:21 |
| 90 | Fabio Duarte | Team Medellin-EPM | + 17:21 | + 17:21 |
| 91 | Davide Bais | EOLO-Kometa Cycling Team | + 17:21 | + 17:21 |
| 92 | Nicolas Cabrera | Chile | + 17:21 | + 17:21 |
| 93 | Giovanni Lonardi | EOLO-Kometa Cycling Team | + 17:21 | + 17:21 |
| 94 | Simone Bevilacqua | EOLO-Kometa Cycling Team | + 17:21 | + 17:21 |
| 95 | Sam Welsford | Team dsm-firmenich | + 17:21 | + 17:21 |
| 96 | Hugo Ruiz | SEP San Juan | + 17:21 | + 17:21 |
| 97 | Maciej Bodnar | TotalEnergies | + 17:21 | + 17:21 |
| 98 | Jon Aberasturi | Lidl-Trek | + 17:21 | + 17:21 |
| 99 | Victor Ocampo | Team Medellin-EPM | + 17:21 | + 17:21 |
| 100 | Luis Rodriguez Jose | Chile | + 17:21 | + 17:21 |
| 101 | Peter Sagan | TotalEnergies | + 17:21 | + 17:21 |
| 102 | Juan Caorsi | Uruguay | + 17:21 | + 17:21 |
| 103 | Roderyck Asconeguy | Uruguay | + 17:21 | + 17:21 |
| 104 | Gerardo Tivani | AV Fatima-San Juan Biker Motos-Electro 3 | + 17:26 | + 17:26 |
| 105 | Agustin Videla | Municipalidad de Rawson | + 21:25 | + 21:25 |
| 106 | Javier Melivillo Juan | EC Municipalidad de Pocito | + 21:25 | + 21:25 |
| 107 | Maximiliano Navarrete | Gremios por el Deporte Cutral Co. | + 21:25 | + 21:25 |
| 108 | Michele Scartezzini | Italy | + 21:25 | + 21:25 |
| 109 | Lukas Dundic | Argentina | + 21:25 | + 21:25 |
| 110 | Gleb Syritsa | Astana Qazaqstan Team | + 21:25 | + 21:25 |
| 111 | Mauro Richeze | Chimbas te Quiero | + 21:25 | + 21:25 |
| 112 | Francesco Lamon | Italy | + 21:25 | + 21:25 |
| 113 | Mattia Pinazzi | Italy | + 21:25 | + 21:25 |
| 114 | Yevgeniy Gidich | Astana Qazaqstan Team | + 21:25 | + 21:25 |
| 115 | Manlio Moro | Italy | + 21:25 | + 21:25 |
| 116 | German Lopez Franco | Argentina | + 21:25 | + 21:25 |
| 117 | Ryan Mullen | BORA-hansgrohe | + 21:25 | + 21:25 |
| 118 | Fernando Gaviria | Movistar Team | + 21:25 | + 21:25 |
| 119 | Yelko Gomez | Panamá es Cultura y Valores | + 21:25 | + 21:25 |
| 120 | Sam Bennett | BORA-hansgrohe | + 21:25 | + 21:25 |
| 121 | Elia Viviani | INEOS Grenadiers | + 21:25 | + 21:25 |
| 122 | Davide Boscaro | Italy | + 21:25 | + 21:25 |
| 123 | Wladimir Martinez | AV Fatima-San Juan Biker Motos-Electro 3 | + 21:25 | + 21:25 |
| 124 | Liam Bertazzo | Italy | + 21:25 | + 21:25 |
| 125 | Maximiliano Richeze | Argentina | + 21:51 | + 21:51 |
| 126 | Manuel Lira Eguiguren | Chile | + 22:20 | + 22:20 |
| 127 | Mauricio Paez | Gremios por el Deporte Cutral Co. | + 23:02 | + 23:02 |
| 128 | Ricardo Dominguez Mauricio | AV Fatima-San Juan Biker Motos-Electro 3 | + 23:02 | + 23:02 |
| 129 | Alejandro Quilci | Gremios por el Deporte Cutral Co. | + 23:28 | + 23:28 |
| 130 | Javier Lopez Federico | EC Municipalidad de Pocito | + 23:28 | + 23:28 |
| 131 | Michael Mørkøv | Soudal Quick-Step | + 24:38 | + 24:38 |
| 132 | Fabio Jakobsen | Soudal Quick-Step | + 24:38 | + 24:38 |
| 133 | Marcos Mendez | Argentina | + 24:38 | + 24:38 |
| 134 | Emiliano Contreras | Chimbas te Quiero | + 24:38 | + 24:38 |
| 135 | Francisco Kotsakis Lagos | Chile | + 31:50 | + 31:50 |
| 136 | Bredio Ruiz | Panamá es Cultura y Valores | + 31:50 | + 31:50 |
| 137 | Cristian Pita | Team Banco Guayaquil | + 43:24 | + 43:24 |