| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Fabian Cancellara | Team CSC - Saxo Bank | 04:34:41 | |
| 2 | Alessandro Ballan | Lampre | + 00 | |
| 3 | Linus Gerdemann | Team Columbia | + 15 | + 15 |
| 4 | Martijn Maaskant | Garmin-Chipotle p/b H30 | + 15 | + 15 |
| 5 | Baden Cooke | Barloworld | + 15 | + 15 |
| 6 | Patrick Calcagni | Barloworld | + 15 | + 15 |
| 7 | Niklas Axelsson | Serramenti - Androni Giocattoli | + 15 | + 15 |
| 8 | Thomas Löfkvist | Team Columbia | + 15 | + 15 |
| 9 | Pavel Brutt | Tinkoff Credit Systems | + 38 | + 38 |
| 10 | Ryder Hesjedal | Garmin-Chipotle p/b H30 | + 38 | + 38 |
| 11 | Giairo Ermeti | LPR Brakes - Ballan | + 38 | + 38 |
| 12 | Paolo Longo Borghini | Barloworld | + 38 | + 38 |
| 13 | Carlo Scognamiglio | Barloworld | + 38 | + 38 |
| 14 | Murilo Fischer | Liquigas | + 01:10 | + 01:10 |
| 15 | Daniele Pietropolli | LPR Brakes - Ballan | + 01:10 | + 01:10 |
| 16 | Steven Cozza | Garmin-Chipotle p/b H30 | + 01:10 | + 01:10 |
| 17 | Ángel Gómez | Scott - American Beef | + 01:10 | + 01:10 |
| 18 | Asle Arvesen Kurt | Team CSC - Saxo Bank | + 01:10 | + 01:10 |
| 19 | Gerald Ciolek | Team Columbia | + 01:10 | + 01:10 |
| 20 | Danilo Hondo | Serramenti - Androni Giocattoli | + 01:10 | + 01:10 |
| 21 | Fabian Wegmann | Gerolsteiner | + 01:10 | + 01:10 |
| 22 | Leonardo Duque | Cofidis | + 01:10 | + 01:10 |
| 23 | Rubens Bertogliati | Scott - American Beef | + 01:10 | + 01:10 |
| 24 | Vincenzo Nibali | Liquigas | + 01:10 | + 01:10 |
| 25 | Matti Breschel | Team CSC - Saxo Bank | + 01:10 | + 01:10 |
| 26 | Volker Ordowski | Gerolsteiner | + 01:50 | + 01:50 |
| 27 | Marco Velo | Team Milram | + 01:50 | + 01:50 |
| 28 | Mauro Santambrogio | Lampre | + 01:50 | + 01:50 |
| 29 | Anders Lund | Team CSC - Saxo Bank | + 01:50 | + 01:50 |
| 30 | Florent Brard | Cofidis | + 01:50 | + 01:50 |
| 31 | Ivan Rovny | Tinkoff Credit Systems | + 01:50 | + 01:50 |
| 32 | Julien Loubet | AG2R La Mondiale | + 01:50 | + 01:50 |
| 33 | Mauro Da Dalto | Liquigas | + 01:50 | + 01:50 |
| 34 | Stuart O'grady | Team CSC - Saxo Bank | + 01:50 | + 01:50 |
| 35 | Chris Sutton | Garmin-Chipotle p/b H30 | + 01:50 | + 01:50 |
| 36 | Emanuele Sella | CSF Group - Navigare | + 01:50 | + 01:50 |
| 37 | Tiziano Dall'antonia | CSF Group - Navigare | + 01:50 | + 01:50 |
| 38 | Giovanni Visconti | Quick Step | + 01:50 | + 01:50 |
| 39 | Francesco Ginanni | Serramenti - Androni Giocattoli | + 01:50 | + 01:50 |
| 40 | Maximiliano Richeze | CSF Group - Navigare | + 01:50 | + 01:50 |
| 41 | Francesco Bellotti | Barloworld | + 02:20 | + 02:20 |
| 42 | Mauro Facci | Quick Step | + 04:10 | + 04:10 |
| 43 | Yuriy Krivtsov | AG2R La Mondiale | + 04:10 | + 04:10 |
| 44 | Mikhail Ignatiev | Tinkoff Credit Systems | + 04:10 | + 04:10 |
| 45 | Matteo Priamo | CSF Group - Navigare | + 05:20 | + 05:20 |
| 46 | Luis Laverde | CSF Group - Navigare | + 05:20 | + 05:20 |
| 47 | Christian Murro | Lampre | + 05:20 | + 05:20 |
| 48 | Jure Golcer | LPR Brakes - Ballan | + 05:20 | + 05:20 |
| 49 | Stephane Augé | Cofidis | + 05:20 | + 05:20 |
| 50 | Kilian Patour | Garmin-Chipotle p/b H30 | + 05:20 | + 05:20 |
| 51 | Giampaolo Cheula | Barloworld | + 05:20 | + 05:20 |
| 52 | Frederik Willems | Liquigas | + 05:20 | + 05:20 |
| 53 | Bert Grabsch | Team Columbia | + 07:15 | + 07:15 |
| 54 | Kanstantsin Sivtsov | Team Columbia | + 07:15 | + 07:15 |
| 55 | Eros Capecchi | Scott - American Beef | + 08:20 | + 08:20 |
| 56 | Ermanno Capelli | Scott - American Beef | + 08:20 | + 08:20 |
| 57 | Raivis Belohvosciks | Scott - American Beef | + 08:20 | + 08:20 |
| 58 | Riccardo Chiarini | LPR Brakes - Ballan | + 08:20 | + 08:20 |
| 59 | Paolo Bailetti | LPR Brakes - Ballan | + 08:20 | + 08:20 |
| 60 | Lars Bak | Team CSC - Saxo Bank | + 09:30 | + 09:30 |
| 61 | William Frischkorn | Garmin-Chipotle p/b H30 | + 09:30 | + 09:30 |
| 62 | Fabio Sabatini | Team Milram | + 10:00 | + 10:00 |
| 63 | Paolo Bettini | Quick Step | + 10:00 | + 10:00 |