| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Thomas Löfkvist | Team Columbia - HTC | 04:59:02 | |
| 2 | Fabian Wegmann | Team Milram | + 04 | + 04 |
| 3 | Martin Elmiger | AG2R La Mondiale | + 06 | + 06 |
| 4 | Edvald Boasson-hagen | Team Columbia - HTC | + 08 | + 08 |
| 5 | Linus Gerdemann | Team Milram | + 12 | + 12 |
| 6 | Giovanni Visconti | ISD - Neri | + 14 | + 14 |
| 7 | Peter Velits | Team Milram | + 14 | + 14 |
| 8 | Andy Schleck | Team Saxo Bank | + 16 | + 16 |
| 9 | Daniel Lloyd | Cervelo TestTeam | + 19 | + 19 |
| 10 | Ryder Hesjedal | Garmin-Slipstream | + 22 | + 22 |
| 11 | Daniele Bennati | Liquigas | + 29 | + 29 |
| 12 | Sergei Ivanov | Team Katusha | + 30 | + 30 |
| 13 | Ignatas Konovalovas | Cervelo TestTeam | + 34 | + 34 |
| 14 | Roger Hammond | Cervelo TestTeam | + 34 | + 34 |
| 15 | Riccardo Chiarini | LPR Brakes - Farnese Vini | + 50 | + 50 |
| 16 | Oscar Gatto | ISD - Neri | + 01:18 | + 01:18 |
| 17 | Daniele Righi | Lampre-NGC | + 01:36 | + 01:36 |
| 18 | Dominique Rollin | Cervelo TestTeam | + 01:36 | + 01:36 |
| 19 | Manuel Belletti | Serramenti - Androni Giocattoli | + 01:36 | + 01:36 |
| 20 | Matti Breschel | Team Saxo Bank | + 01:36 | + 01:36 |
| 21 | Leonardo Giordani | Ceramica Flaminia-Bossini Docce | + 01:36 | + 01:36 |
| 22 | Martijn Maaskant | Garmin-Slipstream | + 01:36 | + 01:36 |
| 23 | Bernhard Eisel | Team Columbia - HTC | + 01:40 | + 01:40 |
| 24 | Mark Cavendish | Team Columbia - HTC | + 01:41 | + 01:41 |
| 25 | Paolo Longo Borghini | Barloworld - Bianchi | + 01:41 | + 01:41 |
| 26 | Francesco Failli | Acqua e Sapone - Caffe Mokambo | + 01:41 | + 01:41 |
| 27 | Luca Mazzanti | Team Katusha | + 01:47 | + 01:47 |
| 28 | Andriy Grivko | ISD - Neri | + 01:47 | + 01:47 |
| 29 | Lorenzo Bernucci | LPR Brakes - Farnese Vini | + 02:14 | + 02:14 |
| 30 | Diego Caccia | Barloworld - Bianchi | + 02:14 | + 02:14 |
| 31 | Andreas Klier | Cervelo TestTeam | + 02:16 | + 02:16 |
| 32 | Alexandr Pliuschin | AG2R La Mondiale | + 02:22 | + 02:22 |
| 33 | Filippo Pozzato | Team Katusha | + 04:20 | + 04:20 |
| 34 | Francesco Ginanni | Serramenti - Androni Giocattoli | + 04:20 | + 04:20 |
| 35 | Michael Albasini | Team Columbia - HTC | + 04:20 | + 04:20 |
| 36 | Dario Cioni | ISD - Neri | + 04:33 | + 04:33 |
| 37 | Marcus Ljungqvist | Team Saxo Bank | + 05:43 | + 05:43 |
| 38 | Manuel Quinziato | Liquigas | + 05:43 | + 05:43 |
| 39 | Serguei Klimov | Team Katusha | + 05:43 | + 05:43 |
| 40 | Oleksandr Kvachuk | ISD - Neri | + 05:43 | + 05:43 |
| 41 | Tyler Farrar | Garmin-Slipstream | + 05:43 | + 05:43 |
| 42 | William Frischkorn | Garmin-Slipstream | + 05:43 | + 05:43 |
| 43 | Gabriel Rasch | Cervelo TestTeam | + 05:43 | + 05:43 |
| 44 | Pavel Brutt | Team Katusha | + 05:43 | + 05:43 |
| 45 | Maxim Belkov | ISD - Neri | + 05:43 | + 05:43 |
| 46 | Svein Tuft | Garmin-Slipstream | + 06:00 | + 06:00 |
| 47 | Chris Sutton | Garmin-Slipstream | + 06:00 | + 06:00 |
| 48 | Stuart O'grady | Team Saxo Bank | + 06:00 | + 06:00 |
| 49 | Maurizio Biondo | Ceramica Flaminia-Bossini Docce | + 06:00 | + 06:00 |
| 50 | Fabio Sabatini | Liquigas | + 09:56 | + 09:56 |
| 51 | Leonardo Scarselli | ISD - Neri | + 09:56 | + 09:56 |
| 52 | Brett Lancaster | Cervelo TestTeam | + 09:56 | + 09:56 |
| 53 | Daniel Oss | Liquigas | + 09:56 | + 09:56 |
| 54 | Luca Pierfelici | Acqua e Sapone - Caffe Mokambo | + 09:56 | + 09:56 |
| 55 | Asle Arvesen Kurt | Team Saxo Bank | + 12:27 | + 12:27 |
| 56 | Martin Reimer | Cervelo TestTeam | + 12:27 | + 12:27 |
| 57 | Kasper Klostergaard | Team Saxo Bank | + 12:27 | + 12:27 |
| 58 | Mauro Da Dalto | Lampre-NGC | + 12:27 | + 12:27 |
| 59 | Jean-charles Senac | AG2R La Mondiale | + 12:27 | + 12:27 |
| 60 | Alberto Ongarato | LPR Brakes - Farnese Vini | + 12:27 | + 12:27 |
| 61 | Mike Friedman | Garmin-Slipstream | + 12:27 | + 12:27 |
| 62 | Julien Loubet | AG2R La Mondiale | + 12:27 | + 12:27 |
| 63 | Vitaliy Buts | Lampre-NGC | + 12:27 | + 12:27 |
| 64 | Ben Swift | Team Katusha | + 12:27 | + 12:27 |
| 65 | Giampaolo Cheula | Barloworld - Bianchi | + 12:27 | + 12:27 |
| 66 | Carlo Scognamiglio | Barloworld - Bianchi | + 12:27 | + 12:27 |
| 67 | Luigi Sestili | Ceramica Flaminia-Bossini Docce | + 13:57 | + 13:57 |
| 68 | Adriano Angeloni | Ceramica Flaminia-Bossini Docce | + 13:57 | + 13:57 |
| 69 | Angel Mate Luis | Serramenti - Androni Giocattoli | + 13:57 | + 13:57 |
| 70 | Fabio Negri | LPR Brakes - Farnese Vini | + 13:57 | + 13:57 |
| 71 | Andrea Masciarelli | Acqua e Sapone - Caffe Mokambo | + 13:57 | + 13:57 |
| 72 | Simone Masciarelli | Acqua e Sapone - Caffe Mokambo | + 13:57 | + 13:57 |