| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Maxim Iglinskiy | Astana | 04:59:48 | |
| 2 | Thomas Löfkvist | Sky Professional Cycling Team | + 01 | + 01 |
| 3 | Michael Rogers | Team HTC - Columbia | + 01 | + 01 |
| 4 | Filippo Pozzato | Team Katusha | + 18 | + 18 |
| 5 | Ryder Hesjedal | Garmin-Transitions | + 19 | + 19 |
| 6 | Francesco Ginanni | Androni Giocattoli | + 24 | + 24 |
| 7 | Leonardo Bertagnolli | Androni Giocattoli | + 43 | + 43 |
| 8 | Antonio Flecha Juan | Sky Professional Cycling Team | + 43 | + 43 |
| 9 | Enrico Gasparotto | Astana | + 49 | + 49 |
| 10 | Daniele Righi | Lampre-Farnese Vini | + 49 | + 49 |
| 11 | Fabian Cancellara | Team Saxo Bank | + 01:19 | + 01:19 |
| 12 | Matti Breschel | Team Saxo Bank | + 01:41 | + 01:41 |
| 13 | Daniel Oss | Liquigas-Doimo | + 01:41 | + 01:41 |
| 14 | Greg van Avermaet | Omega Pharma-Lotto | + 01:41 | + 01:41 |
| 15 | Simon Spilak | Lampre-Farnese Vini | + 01:41 | + 01:41 |
| 16 | Stefano Garzelli | Acqua e Sapone | + 01:41 | + 01:41 |
| 17 | Luca Paolini | Acqua e Sapone | + 01:41 | + 01:41 |
| 18 | Simon Clarke | ISD - Neri | + 03:04 | + 03:04 |
| 19 | Franco Pellizotti | Liquigas-Doimo | + 03:04 | + 03:04 |
| 20 | Assan Bazayev | Astana | + 03:04 | + 03:04 |
| 21 | Peter Velits | Team HTC - Columbia | + 03:04 | + 03:04 |
| 22 | Lorenzo Bernucci | Lampre-Farnese Vini | + 03:04 | + 03:04 |
| 23 | Giovanni Visconti | ISD - Neri | + 03:04 | + 03:04 |
| 24 | Thomas Frei | BMC Racing Team | + 03:40 | + 03:40 |
| 25 | Michael Schär | BMC Racing Team | + 03:40 | + 03:40 |
| 26 | René Mandri | AG2R La Mondiale | + 03:43 | + 03:43 |
| 27 | Massimo Codol | Acqua e Sapone | + 03:43 | + 03:43 |
| 28 | Marco Pinotti | Team HTC - Columbia | + 04:08 | + 04:08 |
| 29 | Bernhard Eisel | Team HTC - Columbia | + 04:08 | + 04:08 |
| 30 | Luca Mazzanti | Team Katusha | + 06:15 | + 06:15 |
| 31 | Carlo Scognamiglio | ISD - Neri | + 06:15 | + 06:15 |
| 32 | Michael Barry | Sky Professional Cycling Team | + 06:15 | + 06:15 |
| 33 | Francesco Failli | Acqua e Sapone | + 06:15 | + 06:15 |
| 34 | Lars Bak | Team HTC - Columbia | + 06:15 | + 06:15 |
| 35 | Nikita Eskov | Team Katusha | + 06:15 | + 06:15 |
| 36 | Paolo Ciavatta | Acqua e Sapone | + 06:15 | + 06:15 |
| 37 | Matthew Wilson | Garmin-Transitions | + 06:15 | + 06:15 |
| 38 | Vincenzo Nibali | Liquigas-Doimo | + 06:15 | + 06:15 |
| 39 | Martijn Maaskant | Garmin-Transitions | + 06:15 | + 06:15 |
| 40 | Paolo Longo Borghini | ISD - Neri | + 06:15 | + 06:15 |
| 41 | Alexander Efimkin | AG2R La Mondiale | + 06:15 | + 06:15 |
| 42 | Alessandro Donati | Acqua e Sapone | + 06:15 | + 06:15 |
| 43 | Kristjan Koren | Liquigas-Doimo | + 06:15 | + 06:15 |
| 44 | Chris Sutton | Sky Professional Cycling Team | + 06:15 | + 06:15 |
| 45 | Gabriel Rasch | Cervelo TestTeam | + 06:15 | + 06:15 |
| 46 | Martin Elmiger | AG2R La Mondiale | + 06:15 | + 06:15 |
| 47 | Allan Davis | Astana | + 06:15 | + 06:15 |
| 48 | Ian Stannard | Sky Professional Cycling Team | + 06:15 | + 06:15 |
| 49 | Ludovic Turpin | AG2R La Mondiale | + 06:15 | + 06:15 |
| 50 | Rubens Bertogliati | Androni Giocattoli | + 06:15 | + 06:15 |
| 51 | Massimo Giunti | Androni Giocattoli | + 06:15 | + 06:15 |
| 52 | Jan Bakelants | Omega Pharma-Lotto | + 06:15 | + 06:15 |
| 53 | Murilo Fischer | Garmin-Transitions | + 06:15 | + 06:15 |
| 54 | Roger Hammond | Cervelo TestTeam | + 06:15 | + 06:15 |
| 55 | Tiziano Dall'antonia | Liquigas-Doimo | + 06:15 | + 06:15 |
| 56 | Cadel Evans | BMC Racing Team | + 06:15 | + 06:15 |
| 57 | Stuart O'grady | Team Saxo Bank | + 06:15 | + 06:15 |
| 58 | Mathew Hayman | Sky Professional Cycling Team | + 06:15 | + 06:15 |
| 59 | Kim Kirchen | Team Katusha | + 06:48 | + 06:48 |
| 60 | Davide Appollonio | Cervelo TestTeam | + 08:12 | + 08:12 |
| 61 | Valeriy Dmitriyev | Astana | + 10:17 | + 10:17 |
| 62 | Andrea Masciarelli | Acqua e Sapone | + 10:17 | + 10:17 |
| 63 | Marco Bandiera | Team Katusha | + 13:30 | + 13:30 |
| 64 | Dmitro Krivtsov | ISD - Neri | + 13:30 | + 13:30 |
| 65 | Brent Bookwalter | BMC Racing Team | + 13:30 | + 13:30 |