| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | José Rujano | Androni Giocattoli - CIPI | 04:45:54 | |
| 2 | Alberto Contador | Saxo Bank - SunGard | + 00 | |
| 3 | John Gadret | AG2R La Mondiale | + 01:27 | + 01:27 |
| 4 | Hubert Dupont | AG2R La Mondiale | + 01:29 | + 01:29 |
| 5 | Igor Anton | Euskaltel-Euskadi | + 01:29 | + 01:29 |
| 6 | Roman Kreuziger | Pro Team Astana | + 01:36 | + 01:36 |
| 7 | Michele Scarponi | Lampre-ISD | + 01:36 | + 01:36 |
| 8 | Vincenzo Nibali | Liquigas-Cannondale | + 01:36 | + 01:36 |
| 9 | Vasil Kiryienka | Movistar Team | + 01:36 | + 01:36 |
| 10 | Denis Menchov | Geox-TMC | + 01:36 | + 01:36 |
| 11 | David Arroyo | Movistar Team | + 01:36 | + 01:36 |
| 12 | Joaquim Rodríguez | Katusha Team | + 01:52 | + 01:52 |
| 13 | Steven Kruijswijk | Rabobank | + 01:52 | + 01:52 |
| 14 | Matteo Carrara | Vacansoleil - DCM Pro Cycling Team | + 02:02 | + 02:02 |
| 15 | Francesco Masciarelli | Pro Team Astana | + 02:14 | + 02:14 |
| 16 | Mikel Nieve | Euskaltel-Euskadi | + 02:28 | + 02:28 |
| 17 | Kanstantsin Sivtsov | Team HTC - Highroad | + 02:42 | + 02:42 |
| 18 | Przemyslaw Niemiec | Lampre-ISD | + 02:42 | + 02:42 |
| 19 | Johann Tschopp | BMC Racing Team | + 02:42 | + 02:42 |
| 20 | Dario Cataldo | Quick Step | + 02:42 | + 02:42 |
| 21 | Emanuele Sella | Androni Giocattoli - CIPI | + 03:13 | + 03:13 |
| 22 | Daniel Moreno | Katusha Team | + 03:13 | + 03:13 |
| 23 | Paolo Tiralongo | Pro Team Astana | + 03:13 | + 03:13 |
| 24 | Tiago Machado | Team RadioShack | + 03:13 | + 03:13 |
| 25 | Peter Stetina | Garmin-Cervélo | + 03:49 | + 03:49 |
| 26 | Christophe Le Mével | Garmin-Cervélo | + 03:49 | + 03:49 |
| 27 | Ivan Rovny | Team RadioShack | + 03:49 | + 03:49 |
| 28 | Dario Cioni | Sky ProCycling | + 03:49 | + 03:49 |
| 29 | Thomas Löfkvist | Sky ProCycling | + 03:49 | + 03:49 |
| 30 | Stefano Garzelli | Acqua e Sapone | + 03:49 | + 03:49 |
| 31 | Marco Pinotti | Team HTC - Highroad | + 03:49 | + 03:49 |
| 32 | Sergio Pardilla | Movistar Team | + 03:49 | + 03:49 |
| 33 | Jesús Hernández | Saxo Bank - SunGard | + 03:49 | + 03:49 |
| 34 | Eros Capecchi | Liquigas-Cannondale | + 03:49 | + 03:49 |
| 35 | Cayetano Sarmiento Jose | Acqua e Sapone | + 03:49 | + 03:49 |
| 36 | Bart de Clercq | Omega Pharma-Lotto | + 04:01 | + 04:01 |
| 37 | José Ochoa Carles | Androni Giocattoli - CIPI | + 04:18 | + 04:18 |
| 38 | Jan Bakelants | Omega Pharma-Lotto | + 04:38 | + 04:38 |
| 39 | Philip Deignan | Team RadioShack | + 05:01 | + 05:01 |
| 40 | Vladimir Miholjevic | Acqua e Sapone | + 05:26 | + 05:26 |
| 41 | Cristiano Salerno | Liquigas-Cannondale | + 05:26 | + 05:26 |
| 42 | Massimo Codol | Acqua e Sapone | + 05:46 | + 05:46 |
| 43 | Stefano Pirazzi | Colnago - CSF Inox | + 06:17 | + 06:17 |
| 44 | Francis de Greef | Omega Pharma-Lotto | + 06:19 | + 06:19 |
| 45 | Pieter Weening | Rabobank | + 06:42 | + 06:42 |
| 46 | Ben Gastauer | AG2R La Mondiale | + 07:21 | + 07:21 |
| 47 | Diego Ulissi | Lampre-ISD | + 07:21 | + 07:21 |
| 48 | Marcel Wyss | Geox-TMC | + 07:26 | + 07:26 |
| 49 | Carlos Sastre | Geox-TMC | + 07:26 | + 07:26 |
| 50 | Kristof Vandewalle | Quick Step | + 07:26 | + 07:26 |
| 51 | Eduard Vorganov | Katusha Team | + 07:26 | + 07:26 |
| 52 | Andrea Noè | Farnese Vini - Neri Sottoli | + 07:26 | + 07:26 |
| 53 | Bram Tankink | Rabobank | + 07:33 | + 07:33 |
| 54 | Alberto Losada | Katusha Team | + 09:11 | + 09:11 |
| 55 | Johnny Hoogerland | Vacansoleil - DCM Pro Cycling Team | + 10:23 | + 10:23 |
| 56 | Cyril Dessel | AG2R La Mondiale | + 10:34 | + 10:34 |
| 57 | Marco Marzano | Lampre-ISD | + 10:51 | + 10:51 |
| 58 | Valerio Agnoli | Liquigas-Cannondale | + 12:24 | + 12:24 |
| 59 | Daniel Navarro | Saxo Bank - SunGard | + 12:24 | + 12:24 |
| 60 | José Serpa | Androni Giocattoli - CIPI | + 12:24 | + 12:24 |
| 61 | Giampaolo Caruso | Katusha Team | + 12:24 | + 12:24 |
| 62 | Petter Nordhaug Lars | Sky ProCycling | + 12:24 | + 12:24 |
| 63 | Sebastian Lang | Omega Pharma-Lotto | + 12:24 | + 12:24 |
| 64 | Sergey Lagutin | Vacansoleil - DCM Pro Cycling Team | + 12:24 | + 12:24 |
| 65 | Filippo Savini | Colnago - CSF Inox | + 12:24 | + 12:24 |
| 66 | Carlos Betancur | Acqua e Sapone | + 12:29 | + 12:29 |
| 67 | Fumiyuki Beppu | Team RadioShack | + 13:17 | + 13:17 |
| 68 | Stef Clement | Rabobank | + 13:17 | + 13:17 |
| 69 | David Blanco | Geox-TMC | + 13:17 | + 13:17 |
| 70 | Michael Barry | Sky ProCycling | + 13:17 | + 13:17 |
| 71 | Joan Horrach | Katusha Team | + 13:17 | + 13:17 |
| 72 | Craig Lewis | Team HTC - Highroad | + 13:17 | + 13:17 |
| 74 | Yaroslav Popovych | Team RadioShack | + 13:48 | + 13:48 |
| 75 | Dmitriy Kozontchuk | Geox-TMC | + 13:48 | + 13:48 |
| 76 | Peter Kennaugh | Sky ProCycling | + 14:01 | + 14:01 |
| 77 | Carlos Oyarzun | Movistar Team | + 14:10 | + 14:10 |
| 78 | Evgeni Petrov | Pro Team Astana | + 14:10 | + 14:10 |
| 79 | Addy Engels | Quick Step | + 14:19 | + 14:19 |
| 80 | Pablo Lastras | Movistar Team | + 14:19 | + 14:19 |
| 81 | David Millar | Garmin-Cervélo | + 14:21 | + 14:21 |
| 82 | Laurent Didier | Saxo Bank - SunGard | + 14:45 | + 14:45 |
| 83 | Luis Pasamontes | Movistar Team | + 14:45 | + 14:45 |
| 84 | Volodymir Gustov | Saxo Bank - SunGard | + 15:50 | + 15:50 |
| 85 | Branislau Samoilau | Movistar Team | + 15:50 | + 15:50 |
| 86 | Morris Possoni | Sky ProCycling | + 15:50 | + 15:50 |
| 87 | Richie Porte | Saxo Bank - SunGard | + 15:50 | + 15:50 |
| 88 | Ignatas Konovalovas | Movistar Team | + 15:50 | + 15:50 |
| 89 | Simone Stortoni | Colnago - CSF Inox | + 15:50 | + 15:50 |
| 90 | Maxim Belkov | Vacansoleil - DCM Pro Cycling Team | + 15:50 | + 15:50 |
| 91 | Robert Kiserlovski | Pro Team Astana | + 15:50 | + 15:50 |
| 92 | Matteo Montaguti | AG2R La Mondiale | + 15:58 | + 15:58 |
| 93 | Giampaolo Cheula | Geox-TMC | + 15:58 | + 15:58 |
| 94 | Angel Vicioso | Androni Giocattoli - CIPI | + 16:00 | + 16:00 |
| 95 | Rinaldo Nocentini | AG2R La Mondiale | + 16:00 | + 16:00 |
| 96 | Jorge Azanza | Euskaltel-Euskadi | + 16:09 | + 16:09 |
| 97 | Bjorn Selander | Team RadioShack | + 16:09 | + 16:09 |
| 98 | Mirko Selvaggi | Vacansoleil - DCM Pro Cycling Team | + 16:09 | + 16:09 |
| 99 | Brett Lancaster | Garmin-Cervélo | + 16:09 | + 16:09 |
| 100 | Aleksandr Kuschynski | Katusha Team | + 16:09 | + 16:09 |
| 101 | Francesco Reda | Quick Step | + 16:09 | + 16:09 |
| 102 | Pavel Brutt | Katusha Team | + 16:09 | + 16:09 |
| 103 | Mikaël Chérel | AG2R La Mondiale | + 16:09 | + 16:09 |
| 104 | Alessandro Vanotti | Liquigas-Cannondale | + 16:09 | + 16:09 |
| 105 | Alessandro Spezialetti | Lampre-ISD | + 16:09 | + 16:09 |
| 106 | Murilo Fischer | Garmin-Cervélo | + 16:15 | + 16:15 |
| 107 | Julien Bérard | AG2R La Mondiale | + 16:15 | + 16:15 |
| 108 | Fabio Taborre | Acqua e Sapone | + 16:15 | + 16:15 |
| 109 | Fredrik Veuchelen | Vacansoleil - DCM Pro Cycling Team | + 16:15 | + 16:15 |
| 110 | Danilo Di Luca | Katusha Team | + 16:18 | + 16:18 |
| 111 | Cameron Meyer | Garmin-Cervélo | + 16:18 | + 16:18 |
| 112 | Simon Zahner | BMC Racing Team | + 18:56 | + 18:56 |
| 113 | Josep Jufré | Pro Team Astana | + 18:56 | + 18:56 |
| 114 | Maxim Gourov | Pro Team Astana | + 18:56 | + 18:56 |
| 115 | Gorazd Stangelj | Pro Team Astana | + 18:56 | + 18:56 |
| 116 | Danilo Wyss | BMC Racing Team | + 18:56 | + 18:56 |
| 117 | Kjell Carlström | Sky ProCycling | + 18:56 | + 18:56 |
| 118 | Rafael Valls | Geox-TMC | + 18:56 | + 18:56 |
| 119 | Simon Spilak | Lampre-ISD | + 18:56 | + 18:56 |
| 120 | Ruggero Marzoli | Acqua e Sapone | + 18:56 | + 18:56 |
| 121 | Michal Golas | Vacansoleil - DCM Pro Cycling Team | + 18:56 | + 18:56 |
| 122 | Daniele Righi | Lampre-ISD | + 19:42 | + 19:42 |
| 123 | Robert Hunter | Team RadioShack | + 20:43 | + 20:43 |
| 124 | Fabio Sabatini | Liquigas-Cannondale | + 20:43 | + 20:43 |
| 125 | Claudio Corioni | Acqua e Sapone | + 20:43 | + 20:43 |
| 127 | Tiziano Dall'antonia | Liquigas-Cannondale | + 20:43 | + 20:43 |
| 128 | Chad Beyer | BMC Racing Team | + 20:43 | + 20:43 |
| 129 | Lars Bak | Team HTC - Highroad | + 20:43 | + 20:43 |
| 130 | Oscar Gatto | Farnese Vini - Neri Sottoli | + 20:43 | + 20:43 |
| 131 | Patrick Gretsch | Team HTC - Highroad | + 20:43 | + 20:43 |
| 132 | Alan Marangoni | Liquigas-Cannondale | + 20:43 | + 20:43 |
| 133 | Gert Dockx | Omega Pharma-Lotto | + 20:43 | + 20:43 |
| 134 | José Oroz Juan | Euskaltel-Euskadi | + 20:43 | + 20:43 |
| 135 | Sylwester Szmyd | Liquigas-Cannondale | + 20:43 | + 20:43 |
| 136 | Iñaki Isasi | Euskaltel-Euskadi | + 20:43 | + 20:43 |
| 137 | Olivier Kaisen | Omega Pharma-Lotto | + 20:43 | + 20:43 |
| 138 | Dennis van Winden | Rabobank | + 20:43 | + 20:43 |
| 139 | Luca Mazzanti | Farnese Vini - Neri Sottoli | + 20:43 | + 20:43 |
| 140 | Mauricio Ardila | Geox-TMC | + 20:43 | + 20:43 |
| 141 | Federico Canuti | Colnago - CSF Inox | + 20:43 | + 20:43 |
| 142 | Klaas Lodewyck | Omega Pharma-Lotto | + 20:43 | + 20:43 |
| 143 | Yuriy Krivtsov | AG2R La Mondiale | + 20:43 | + 20:43 |
| 144 | Matteo Tosatto | Saxo Bank - SunGard | + 20:43 | + 20:43 |
| 145 | Rick Flens | Rabobank | + 20:43 | + 20:43 |
| 146 | Domenico Pozzovivo | Colnago - CSF Inox | + 20:43 | + 20:43 |
| 147 | Daniel Sesma Sorbet | Euskaltel-Euskadi | + 20:43 | + 20:43 |
| 148 | Jussi Veikkanen | Omega Pharma-Lotto | + 20:43 | + 20:43 |
| 149 | Jos van Emden | Rabobank | + 20:43 | + 20:43 |
| 150 | Mathias Frank | BMC Racing Team | + 20:43 | + 20:43 |
| 151 | Kevin Seeldraeyers | Quick Step | + 20:43 | + 20:43 |
| 152 | Davide Ricci Bitti | Farnese Vini - Neri Sottoli | + 20:43 | + 20:43 |
| 153 | Giovanni Visconti | Farnese Vini - Neri Sottoli | + 20:43 | + 20:43 |
| 154 | Matteo Rabottini | Farnese Vini - Neri Sottoli | + 20:43 | + 20:43 |
| 155 | Pierre Cazaux | Euskaltel-Euskadi | + 20:43 | + 20:43 |
| 156 | Alberto Ongarato | Vacansoleil - DCM Pro Cycling Team | + 20:43 | + 20:43 |
| 157 | Jérôme Pineau | Quick Step | + 20:43 | + 20:43 |
| 158 | Roberto Ferrari | Androni Giocattoli - CIPI | + 20:57 | + 20:57 |
| 159 | Alessandro de Marchi | Androni Giocattoli - CIPI | + 20:57 | + 20:57 |
| 160 | Alexsandr Dyachenko | Pro Team Astana | + 20:57 | + 20:57 |
| 161 | Leonardo Giordani | Farnese Vini - Neri Sottoli | + 20:57 | + 20:57 |
| 162 | Giairo Ermeti | Androni Giocattoli - CIPI | + 20:57 | + 20:57 |
| 163 | Manuel Cardoso | Team RadioShack | + 21:21 | + 21:21 |
| 164 | Alex Rasmussen | Team HTC - Highroad | + 23:25 | + 23:25 |
| 165 | Michael Mørkøv | Saxo Bank - SunGard | + 23:25 | + 23:25 |
| 166 | Javier Aramendia | Euskaltel-Euskadi | + 23:25 | + 23:25 |
| 167 | Matthew Wilson | Garmin-Cervélo | + 23:25 | + 23:25 |
| 168 | Davide Malacarne | Quick Step | + 23:25 | + 23:25 |
| 169 | Alexander Kristoff | BMC Racing Team | + 23:25 | + 23:25 |
| 170 | Kasper Klostergaard | Saxo Bank - SunGard | + 23:25 | + 23:25 |
| 171 | Gianluca Brambilla | Colnago - CSF Inox | + 23:25 | + 23:25 |
| 172 | Miguel Minguez | Euskaltel-Euskadi | + 23:25 | + 23:25 |
| 173 | Francesco Chicchi | Quick Step | + 23:25 | + 23:25 |
| 174 | Russell Downing | Sky ProCycling | + 23:25 | + 23:25 |
| 175 | Elia Favilli | Farnese Vini - Neri Sottoli | + 23:51 | + 23:51 |
| 176 | Frantisek Rabon | Team HTC - Highroad | + 23:54 | + 23:54 |