| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Igor Anton | Euskaltel-Euskadi | 05:04:26 | |
| 2 | Alberto Contador | Saxo Bank - SunGard | + 33 | + 33 |
| 3 | Vincenzo Nibali | Liquigas-Cannondale | + 40 | + 40 |
| 4 | Michele Scarponi | Lampre-ISD | + 01:11 | + 01:11 |
| 5 | Denis Menchov | Geox-TMC | + 01:21 | + 01:21 |
| 6 | John Gadret | AG2R La Mondiale | + 01:38 | + 01:38 |
| 7 | Mikel Nieve | Euskaltel-Euskadi | + 01:52 | + 01:52 |
| 8 | Hubert Dupont | AG2R La Mondiale | + 01:55 | + 01:55 |
| 9 | Kanstantsin Sivtsov | Team HTC - Highroad | + 02:05 | + 02:05 |
| 10 | José Rujano | Androni Giocattoli - CIPI | + 02:11 | + 02:11 |
| 11 | Joaquim Rodríguez | Katusha Team | + 02:24 | + 02:24 |
| 12 | Steven Kruijswijk | Rabobank | + 02:40 | + 02:40 |
| 13 | Przemyslaw Niemiec | Lampre-ISD | + 02:57 | + 02:57 |
| 14 | Paolo Tiralongo | Pro Team Astana | + 03:29 | + 03:29 |
| 15 | Peter Stetina | Garmin-Cervélo | + 03:29 | + 03:29 |
| 16 | Roman Kreuziger | Pro Team Astana | + 03:32 | + 03:32 |
| 17 | David Arroyo | Movistar Team | + 03:39 | + 03:39 |
| 18 | Matteo Carrara | Vacansoleil - DCM Pro Cycling Team | + 03:55 | + 03:55 |
| 19 | Christophe Le Mével | Garmin-Cervélo | + 04:00 | + 04:00 |
| 20 | Dario Cataldo | Quick Step | + 04:03 | + 04:03 |
| 21 | Tiago Machado | Team RadioShack | + 04:03 | + 04:03 |
| 22 | Vasil Kiryienka | Movistar Team | + 04:30 | + 04:30 |
| 23 | Marcel Wyss | Geox-TMC | + 05:05 | + 05:05 |
| 24 | Jan Bakelants | Omega Pharma-Lotto | + 05:07 | + 05:07 |
| 25 | Ivan Rovny | Team RadioShack | + 05:07 | + 05:07 |
| 26 | Emanuele Sella | Androni Giocattoli - CIPI | + 05:18 | + 05:18 |
| 27 | Jesús Hernández | Saxo Bank - SunGard | + 05:23 | + 05:23 |
| 28 | Vladimir Miholjevic | Acqua e Sapone | + 05:27 | + 05:27 |
| 29 | Giovanni Visconti | Farnese Vini - Neri Sottoli | + 05:27 | + 05:27 |
| 30 | Daniel Moreno | Katusha Team | + 05:32 | + 05:32 |
| 31 | Sergio Pardilla | Movistar Team | + 05:53 | + 05:53 |
| 32 | Bram Tankink | Rabobank | + 05:54 | + 05:54 |
| 33 | Diego Ulissi | Lampre-ISD | + 05:54 | + 05:54 |
| 34 | Rinaldo Nocentini | AG2R La Mondiale | + 05:54 | + 05:54 |
| 35 | Marco Pinotti | Team HTC - Highroad | + 05:54 | + 05:54 |
| 36 | Thomas Löfkvist | Sky ProCycling | + 06:04 | + 06:04 |
| 37 | Johann Tschopp | BMC Racing Team | + 06:04 | + 06:04 |
| 38 | Matteo Rabottini | Farnese Vini - Neri Sottoli | + 06:43 | + 06:43 |
| 39 | Eduard Vorganov | Katusha Team | + 06:57 | + 06:57 |
| 40 | Francis de Greef | Omega Pharma-Lotto | + 07:17 | + 07:17 |
| 41 | Danilo Di Luca | Katusha Team | + 07:26 | + 07:26 |
| 42 | Dario Cioni | Sky ProCycling | + 07:57 | + 07:57 |
| 43 | Jérôme Pineau | Quick Step | + 08:06 | + 08:06 |
| 44 | Bart de Clercq | Omega Pharma-Lotto | + 08:10 | + 08:10 |
| 45 | Matteo Montaguti | AG2R La Mondiale | + 08:35 | + 08:35 |
| 46 | Maxim Belkov | Vacansoleil - DCM Pro Cycling Team | + 09:03 | + 09:03 |
| 47 | Carlos Sastre | Geox-TMC | + 09:14 | + 09:14 |
| 48 | Johnny Hoogerland | Vacansoleil - DCM Pro Cycling Team | + 09:54 | + 09:54 |
| 49 | Robert Kiserlovski | Pro Team Astana | + 10:08 | + 10:08 |
| 50 | Evgeni Petrov | Pro Team Astana | + 10:14 | + 10:14 |
| 51 | Jorge Azanza | Euskaltel-Euskadi | + 10:29 | + 10:29 |
| 52 | Gianluca Brambilla | Colnago - CSF Inox | + 11:24 | + 11:24 |
| 53 | Aleksandr Kuschynski | Katusha Team | + 11:27 | + 11:27 |
| 54 | Gorazd Stangelj | Pro Team Astana | + 11:27 | + 11:27 |
| 55 | Stefano Pirazzi | Colnago - CSF Inox | + 11:32 | + 11:32 |
| 56 | Massimo Codol | Acqua e Sapone | + 11:58 | + 11:58 |
| 57 | Fumiyuki Beppu | Team RadioShack | + 12:07 | + 12:07 |
| 58 | Cayetano Sarmiento Jose | Acqua e Sapone | + 12:10 | + 12:10 |
| 59 | Sebastian Lang | Omega Pharma-Lotto | + 12:12 | + 12:12 |
| 60 | Stef Clement | Rabobank | + 12:12 | + 12:12 |
| 61 | Giampaolo Caruso | Katusha Team | + 12:23 | + 12:23 |
| 62 | Cristiano Salerno | Liquigas-Cannondale | + 12:30 | + 12:30 |
| 63 | Domenico Pozzovivo | Colnago - CSF Inox | + 12:32 | + 12:32 |
| 64 | Francesco Masciarelli | Pro Team Astana | + 12:41 | + 12:41 |
| 65 | Daniel Navarro | Saxo Bank - SunGard | + 12:56 | + 12:56 |
| 66 | David Millar | Garmin-Cervélo | + 12:58 | + 12:58 |
| 67 | Valerio Agnoli | Liquigas-Cannondale | + 13:05 | + 13:05 |
| 68 | Eros Capecchi | Liquigas-Cannondale | + 13:05 | + 13:05 |
| 69 | Gert Dockx | Omega Pharma-Lotto | + 13:15 | + 13:15 |
| 70 | Alessandro Spezialetti | Lampre-ISD | + 13:29 | + 13:29 |
| 71 | Yaroslav Popovych | Team RadioShack | + 13:32 | + 13:32 |
| 72 | José Ochoa Carles | Androni Giocattoli - CIPI | + 13:34 | + 13:34 |
| 73 | Joan Horrach | Katusha Team | + 13:36 | + 13:36 |
| 74 | Patrick Gretsch | Team HTC - Highroad | + 13:39 | + 13:39 |
| 76 | Petter Nordhaug Lars | Sky ProCycling | + 14:25 | + 14:25 |
| 77 | Michael Barry | Sky ProCycling | + 14:52 | + 14:52 |
| 78 | Peter Kennaugh | Sky ProCycling | + 14:54 | + 14:54 |
| 79 | Morris Possoni | Sky ProCycling | + 14:54 | + 14:54 |
| 80 | Cyril Dessel | AG2R La Mondiale | + 14:54 | + 14:54 |
| 81 | Josep Jufré | Pro Team Astana | + 14:54 | + 14:54 |
| 82 | Philip Deignan | Team RadioShack | + 14:54 | + 14:54 |
| 83 | Daniele Righi | Lampre-ISD | + 15:14 | + 15:14 |
| 85 | Ben Gastauer | AG2R La Mondiale | + 15:32 | + 15:32 |
| 86 | David Blanco | Geox-TMC | + 15:32 | + 15:32 |
| 87 | Filippo Savini | Colnago - CSF Inox | + 15:36 | + 15:36 |
| 88 | Carlos Betancur | Acqua e Sapone | + 15:46 | + 15:46 |
| 89 | Iñaki Isasi | Euskaltel-Euskadi | + 16:13 | + 16:13 |
| 90 | Richie Porte | Saxo Bank - SunGard | + 16:13 | + 16:13 |
| 91 | Kjell Carlström | Sky ProCycling | + 16:13 | + 16:13 |
| 92 | Dmitriy Kozontchuk | Geox-TMC | + 16:13 | + 16:13 |
| 93 | Pablo Lastras | Movistar Team | + 16:13 | + 16:13 |
| 94 | Bjorn Selander | Team RadioShack | + 16:13 | + 16:13 |
| 95 | Alexsandr Dyachenko | Pro Team Astana | + 16:20 | + 16:20 |
| 96 | José Serpa | Androni Giocattoli - CIPI | + 16:22 | + 16:22 |
| 97 | Sylwester Szmyd | Liquigas-Cannondale | + 16:26 | + 16:26 |
| 98 | Yuriy Krivtsov | AG2R La Mondiale | + 16:33 | + 16:33 |
| 99 | Federico Canuti | Colnago - CSF Inox | + 16:36 | + 16:36 |
| 100 | Addy Engels | Quick Step | + 16:37 | + 16:37 |
| 101 | Craig Lewis | Team HTC - Highroad | + 16:40 | + 16:40 |
| 102 | Mauricio Ardila | Geox-TMC | + 16:46 | + 16:46 |
| 103 | Luca Mazzanti | Farnese Vini - Neri Sottoli | + 17:02 | + 17:02 |
| 104 | Stefano Garzelli | Acqua e Sapone | + 17:12 | + 17:12 |
| 105 | Maxim Gourov | Pro Team Astana | + 17:12 | + 17:12 |
| 106 | Fabio Sabatini | Liquigas-Cannondale | + 17:12 | + 17:12 |
| 107 | Alessandro Vanotti | Liquigas-Cannondale | + 17:12 | + 17:12 |
| 108 | Branislau Samoilau | Movistar Team | + 17:12 | + 17:12 |
| 109 | Leonardo Giordani | Farnese Vini - Neri Sottoli | + 17:12 | + 17:12 |
| 110 | Angel Vicioso | Androni Giocattoli - CIPI | + 17:12 | + 17:12 |
| 111 | Michal Golas | Vacansoleil - DCM Pro Cycling Team | + 17:12 | + 17:12 |
| 112 | Davide Malacarne | Quick Step | + 17:12 | + 17:12 |
| 113 | Pieter Weening | Rabobank | + 17:37 | + 17:37 |
| 114 | Luis Pasamontes | Movistar Team | + 17:41 | + 17:41 |
| 115 | Alberto Losada | Katusha Team | + 17:41 | + 17:41 |
| 116 | Javier Aramendia | Euskaltel-Euskadi | + 17:50 | + 17:50 |
| 117 | Olivier Kaisen | Omega Pharma-Lotto | + 18:08 | + 18:08 |
| 118 | Jussi Veikkanen | Omega Pharma-Lotto | + 18:11 | + 18:11 |
| 119 | Jos van Emden | Rabobank | + 18:11 | + 18:11 |
| 120 | Simon Zahner | BMC Racing Team | + 18:11 | + 18:11 |
| 121 | Mathias Frank | BMC Racing Team | + 18:14 | + 18:14 |
| 122 | Oscar Gatto | Farnese Vini - Neri Sottoli | + 18:14 | + 18:14 |
| 123 | Davide Ricci Bitti | Farnese Vini - Neri Sottoli | + 18:14 | + 18:14 |
| 124 | Mikaël Chérel | AG2R La Mondiale | + 18:14 | + 18:14 |
| 125 | Simone Stortoni | Colnago - CSF Inox | + 18:14 | + 18:14 |
| 126 | Robert Hunter | Team RadioShack | + 18:14 | + 18:14 |
| 127 | Alan Marangoni | Liquigas-Cannondale | + 18:19 | + 18:19 |
| 128 | Kristof Vandewalle | Quick Step | + 18:19 | + 18:19 |
| 129 | Russell Downing | Sky ProCycling | + 18:19 | + 18:19 |
| 130 | Lars Bak | Team HTC - Highroad | + 18:28 | + 18:28 |
| 131 | Rick Flens | Rabobank | + 18:28 | + 18:28 |
| 132 | Alessandro de Marchi | Androni Giocattoli - CIPI | + 18:37 | + 18:37 |
| 133 | Carlos Oyarzun | Movistar Team | + 18:39 | + 18:39 |
| 134 | Danilo Wyss | BMC Racing Team | + 18:39 | + 18:39 |
| 135 | Julien Bérard | AG2R La Mondiale | + 18:39 | + 18:39 |
| 136 | Pavel Brutt | Katusha Team | + 18:39 | + 18:39 |
| 137 | Klaas Lodewyck | Omega Pharma-Lotto | + 18:46 | + 18:46 |
| 138 | Ignatas Konovalovas | Movistar Team | + 18:50 | + 18:50 |
| 139 | Dennis van Winden | Rabobank | + 18:56 | + 18:56 |
| 140 | Volodymir Gustov | Saxo Bank - SunGard | + 18:56 | + 18:56 |
| 141 | Brett Lancaster | Garmin-Cervélo | + 18:56 | + 18:56 |
| 142 | José Oroz Juan | Euskaltel-Euskadi | + 19:17 | + 19:17 |
| 143 | Claudio Corioni | Acqua e Sapone | + 19:17 | + 19:17 |
| 144 | Pierre Cazaux | Euskaltel-Euskadi | + 19:17 | + 19:17 |
| 145 | Murilo Fischer | Garmin-Cervélo | + 19:20 | + 19:20 |
| 146 | Simon Spilak | Lampre-ISD | + 19:20 | + 19:20 |
| 147 | Alberto Ongarato | Vacansoleil - DCM Pro Cycling Team | + 19:23 | + 19:23 |
| 148 | Francesco Reda | Quick Step | + 19:25 | + 19:25 |
| 149 | Tiziano Dall'antonia | Liquigas-Cannondale | + 19:27 | + 19:27 |
| 150 | Fredrik Veuchelen | Vacansoleil - DCM Pro Cycling Team | + 19:32 | + 19:32 |
| 151 | Elia Favilli | Farnese Vini - Neri Sottoli | + 19:42 | + 19:42 |
| 152 | Marco Marzano | Lampre-ISD | + 19:51 | + 19:51 |
| 153 | Giampaolo Cheula | Geox-TMC | + 20:01 | + 20:01 |
| 154 | Giairo Ermeti | Androni Giocattoli - CIPI | + 20:16 | + 20:16 |
| 155 | Roberto Ferrari | Androni Giocattoli - CIPI | + 20:19 | + 20:19 |
| 156 | Ruggero Marzoli | Acqua e Sapone | + 20:19 | + 20:19 |
| 157 | Fabio Taborre | Acqua e Sapone | + 20:28 | + 20:28 |
| 158 | Matthew Wilson | Garmin-Cervélo | + 20:28 | + 20:28 |
| 159 | Mirko Selvaggi | Vacansoleil - DCM Pro Cycling Team | + 20:48 | + 20:48 |
| 160 | Chad Beyer | BMC Racing Team | + 20:48 | + 20:48 |
| 161 | Sergey Lagutin | Vacansoleil - DCM Pro Cycling Team | + 20:55 | + 20:55 |
| 162 | Daniel Sesma Sorbet | Euskaltel-Euskadi | + 20:55 | + 20:55 |
| 163 | Alex Rasmussen | Team HTC - Highroad | + 21:10 | + 21:10 |
| 164 | Frantisek Rabon | Team HTC - Highroad | + 21:10 | + 21:10 |
| 165 | Matteo Tosatto | Saxo Bank - SunGard | + 21:49 | + 21:49 |
| 166 | Manuel Cardoso | Team RadioShack | + 21:49 | + 21:49 |
| 167 | Michael Mørkøv | Saxo Bank - SunGard | + 21:49 | + 21:49 |
| 168 | Kasper Klostergaard | Saxo Bank - SunGard | + 21:49 | + 21:49 |
| 169 | Laurent Didier | Saxo Bank - SunGard | + 21:49 | + 21:49 |
| 170 | Cameron Meyer | Garmin-Cervélo | + 21:49 | + 21:49 |
| 171 | Miguel Minguez | Euskaltel-Euskadi | + 21:49 | + 21:49 |
| 172 | Alexander Kristoff | BMC Racing Team | + 22:23 | + 22:23 |
| 173 | Kevin Seeldraeyers | Quick Step | + 26:54 | + 26:54 |