| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Paolo Tiralongo | Pro Team Astana | 05:26:27 | |
| 2 | Alberto Contador | Saxo Bank - SunGard | + 00 | |
| 3 | Vincenzo Nibali | Liquigas-Cannondale | + 03 | + 03 |
| 4 | John Gadret | AG2R La Mondiale | + 06 | + 06 |
| 5 | Joaquim Rodríguez | Katusha Team | + 06 | + 06 |
| 6 | Steven Kruijswijk | Rabobank | + 06 | + 06 |
| 7 | Michele Scarponi | Lampre-ISD | + 08 | + 08 |
| 8 | Roman Kreuziger | Pro Team Astana | + 21 | + 21 |
| 9 | Hubert Dupont | AG2R La Mondiale | + 29 | + 29 |
| 10 | Kanstantsin Sivtsov | Team HTC - Highroad | + 34 | + 34 |
| 11 | Mikel Nieve | Euskaltel-Euskadi | + 34 | + 34 |
| 12 | Dario Cataldo | Quick Step | + 40 | + 40 |
| 13 | Daniel Moreno | Katusha Team | + 56 | + 56 |
| 14 | Sergey Lagutin | Vacansoleil - DCM Pro Cycling Team | + 01:00 | + 01:00 |
| 15 | Denis Menchov | Geox-TMC | + 01:01 | + 01:01 |
| 16 | Tiago Machado | Team RadioShack | + 01:04 | + 01:04 |
| 17 | Francis de Greef | Omega Pharma-Lotto | + 01:09 | + 01:09 |
| 18 | David Arroyo | Movistar Team | + 01:15 | + 01:15 |
| 19 | Daniel Navarro | Saxo Bank - SunGard | + 01:20 | + 01:20 |
| 20 | Richie Porte | Saxo Bank - SunGard | + 01:20 | + 01:20 |
| 21 | Mikaël Chérel | AG2R La Mondiale | + 01:22 | + 01:22 |
| 22 | Matteo Carrara | Vacansoleil - DCM Pro Cycling Team | + 01:24 | + 01:24 |
| 23 | José Rujano | Androni Giocattoli - CIPI | + 02:20 | + 02:20 |
| 24 | Branislau Samoilau | Movistar Team | + 02:20 | + 02:20 |
| 25 | Evgeni Petrov | Pro Team Astana | + 02:20 | + 02:20 |
| 26 | Cayetano Sarmiento Jose | Acqua e Sapone | + 02:20 | + 02:20 |
| 27 | Pablo Lastras | Movistar Team | + 02:20 | + 02:20 |
| 29 | Christophe Le Mével | Garmin-Cervélo | + 02:20 | + 02:20 |
| 30 | Peter Stetina | Garmin-Cervélo | + 02:20 | + 02:20 |
| 31 | Sylwester Szmyd | Liquigas-Cannondale | + 02:25 | + 02:25 |
| 32 | Jesús Hernández | Saxo Bank - SunGard | + 02:25 | + 02:25 |
| 33 | Bart de Clercq | Omega Pharma-Lotto | + 02:25 | + 02:25 |
| 34 | Johann Tschopp | BMC Racing Team | + 02:58 | + 02:58 |
| 35 | Thomas Löfkvist | Sky ProCycling | + 03:17 | + 03:17 |
| 36 | David Blanco | Geox-TMC | + 04:29 | + 04:29 |
| 37 | Eduard Vorganov | Katusha Team | + 04:29 | + 04:29 |
| 38 | Bram Tankink | Rabobank | + 05:28 | + 05:28 |
| 39 | Fumiyuki Beppu | Team RadioShack | + 06:00 | + 06:00 |
| 40 | Rinaldo Nocentini | AG2R La Mondiale | + 06:52 | + 06:52 |
| 41 | Giovanni Visconti | Farnese Vini - Neri Sottoli | + 06:52 | + 06:52 |
| 42 | Alessandro Vanotti | Liquigas-Cannondale | + 07:09 | + 07:09 |
| 43 | Matteo Rabottini | Farnese Vini - Neri Sottoli | + 07:09 | + 07:09 |
| 44 | Danilo Di Luca | Katusha Team | + 07:09 | + 07:09 |
| 45 | Gorazd Stangelj | Pro Team Astana | + 07:09 | + 07:09 |
| 46 | Giampaolo Cheula | Geox-TMC | + 07:30 | + 07:30 |
| 47 | Marcel Wyss | Geox-TMC | + 07:30 | + 07:30 |
| 48 | Alberto Losada | Katusha Team | + 08:46 | + 08:46 |
| 49 | Dmitriy Kozontchuk | Geox-TMC | + 10:32 | + 10:32 |
| 50 | Stefano Garzelli | Acqua e Sapone | + 11:01 | + 11:01 |
| 51 | Diego Ulissi | Lampre-ISD | + 11:07 | + 11:07 |
| 52 | Giairo Ermeti | Androni Giocattoli - CIPI | + 13:17 | + 13:17 |
| 53 | Jérôme Pineau | Quick Step | + 14:53 | + 14:53 |
| 54 | Sebastian Lang | Omega Pharma-Lotto | + 15:45 | + 15:45 |
| 55 | Jan Bakelants | Omega Pharma-Lotto | + 15:45 | + 15:45 |
| 56 | Vladimir Miholjevic | Acqua e Sapone | + 15:45 | + 15:45 |
| 57 | Cristiano Salerno | Liquigas-Cannondale | + 15:45 | + 15:45 |
| 58 | Maxim Belkov | Vacansoleil - DCM Pro Cycling Team | + 15:45 | + 15:45 |
| 59 | Simone Stortoni | Colnago - CSF Inox | + 15:45 | + 15:45 |
| 60 | Lars Bak | Team HTC - Highroad | + 15:45 | + 15:45 |
| 61 | Daniele Righi | Lampre-ISD | + 15:45 | + 15:45 |
| 62 | Peter Kennaugh | Sky ProCycling | + 15:53 | + 15:53 |
| 63 | Petter Nordhaug Lars | Sky ProCycling | + 19:31 | + 19:31 |
| 64 | Igor Anton | Euskaltel-Euskadi | + 19:31 | + 19:31 |
| 65 | Iñaki Isasi | Euskaltel-Euskadi | + 19:31 | + 19:31 |
| 66 | Michael Barry | Sky ProCycling | + 19:31 | + 19:31 |
| 67 | Joan Horrach | Katusha Team | + 19:31 | + 19:31 |
| 68 | Luis Pasamontes | Movistar Team | + 19:31 | + 19:31 |
| 69 | Przemyslaw Niemiec | Lampre-ISD | + 19:31 | + 19:31 |
| 70 | Volodymir Gustov | Saxo Bank - SunGard | + 19:31 | + 19:31 |
| 71 | Sergio Pardilla | Movistar Team | + 19:31 | + 19:31 |
| 72 | Dario Cioni | Sky ProCycling | + 19:31 | + 19:31 |
| 73 | Yuriy Krivtsov | AG2R La Mondiale | + 19:31 | + 19:31 |
| 74 | Giampaolo Caruso | Katusha Team | + 19:31 | + 19:31 |
| 75 | Simon Spilak | Lampre-ISD | + 19:31 | + 19:31 |
| 76 | Robert Kiserlovski | Pro Team Astana | + 19:31 | + 19:31 |
| 77 | Danilo Wyss | BMC Racing Team | + 19:31 | + 19:31 |
| 78 | Mathias Frank | BMC Racing Team | + 19:31 | + 19:31 |
| 79 | Johnny Hoogerland | Vacansoleil - DCM Pro Cycling Team | + 19:31 | + 19:31 |
| 80 | Aleksandr Kuschynski | Katusha Team | + 19:31 | + 19:31 |
| 81 | Luca Mazzanti | Farnese Vini - Neri Sottoli | + 20:19 | + 20:19 |
| 82 | Ignatas Konovalovas | Movistar Team | + 20:19 | + 20:19 |
| 83 | Massimo Codol | Acqua e Sapone | + 20:19 | + 20:19 |
| 84 | Federico Canuti | Colnago - CSF Inox | + 20:19 | + 20:19 |
| 85 | Alessandro Spezialetti | Lampre-ISD | + 20:19 | + 20:19 |
| 86 | Fabio Sabatini | Liquigas-Cannondale | + 20:19 | + 20:19 |
| 87 | Davide Malacarne | Quick Step | + 20:19 | + 20:19 |
| 89 | Morris Possoni | Sky ProCycling | + 20:19 | + 20:19 |
| 90 | Pavel Brutt | Katusha Team | + 20:19 | + 20:19 |
| 91 | Jussi Veikkanen | Omega Pharma-Lotto | + 20:19 | + 20:19 |
| 92 | Josep Jufré | Pro Team Astana | + 20:19 | + 20:19 |
| 93 | Pierre Cazaux | Euskaltel-Euskadi | + 20:19 | + 20:19 |
| 94 | Jorge Azanza | Euskaltel-Euskadi | + 20:19 | + 20:19 |
| 95 | Stefano Pirazzi | Colnago - CSF Inox | + 20:19 | + 20:19 |
| 96 | David Millar | Garmin-Cervélo | + 20:19 | + 20:19 |
| 97 | Kristof Vandewalle | Quick Step | + 20:19 | + 20:19 |
| 98 | Carlos Oyarzun | Movistar Team | + 20:19 | + 20:19 |
| 99 | Ben Gastauer | AG2R La Mondiale | + 20:19 | + 20:19 |
| 100 | Fredrik Veuchelen | Vacansoleil - DCM Pro Cycling Team | + 20:19 | + 20:19 |
| 101 | Carlos Sastre | Geox-TMC | + 20:19 | + 20:19 |
| 102 | Carlos Betancur | Acqua e Sapone | + 20:19 | + 20:19 |
| 103 | Cyril Dessel | AG2R La Mondiale | + 20:19 | + 20:19 |
| 104 | Tiziano Dall'antonia | Liquigas-Cannondale | + 20:19 | + 20:19 |
| 105 | Matteo Tosatto | Saxo Bank - SunGard | + 20:19 | + 20:19 |
| 106 | Emanuele Sella | Androni Giocattoli - CIPI | + 20:19 | + 20:19 |
| 107 | Vasil Kiryienka | Movistar Team | + 20:19 | + 20:19 |
| 108 | Francesco Reda | Quick Step | + 20:19 | + 20:19 |
| 109 | Kevin Seeldraeyers | Quick Step | + 20:19 | + 20:19 |
| 110 | Matteo Montaguti | AG2R La Mondiale | + 20:19 | + 20:19 |
| 111 | Pieter Weening | Rabobank | + 20:19 | + 20:19 |
| 112 | Addy Engels | Quick Step | + 20:19 | + 20:19 |
| 113 | Philip Deignan | Team RadioShack | + 20:19 | + 20:19 |
| 114 | Cameron Meyer | Garmin-Cervélo | + 20:19 | + 20:19 |
| 115 | Laurent Didier | Saxo Bank - SunGard | + 20:19 | + 20:19 |
| 116 | Alessandro de Marchi | Androni Giocattoli - CIPI | + 21:22 | + 21:22 |
| 117 | Angel Vicioso | Androni Giocattoli - CIPI | + 21:55 | + 21:55 |
| 118 | Roberto Ferrari | Androni Giocattoli - CIPI | + 21:55 | + 21:55 |
| 119 | Stef Clement | Rabobank | + 26:26 | + 26:26 |
| 120 | Valerio Agnoli | Liquigas-Cannondale | + 26:26 | + 26:26 |
| 121 | Michael Mørkøv | Saxo Bank - SunGard | + 26:26 | + 26:26 |
| 122 | Klaas Lodewyck | Omega Pharma-Lotto | + 26:26 | + 26:26 |
| 123 | Alan Marangoni | Liquigas-Cannondale | + 26:26 | + 26:26 |
| 124 | Claudio Corioni | Acqua e Sapone | + 26:26 | + 26:26 |
| 125 | Kjell Carlström | Sky ProCycling | + 26:26 | + 26:26 |
| 126 | Davide Ricci Bitti | Farnese Vini - Neri Sottoli | + 26:26 | + 26:26 |
| 127 | Oscar Gatto | Farnese Vini - Neri Sottoli | + 26:26 | + 26:26 |
| 128 | Rick Flens | Rabobank | + 26:26 | + 26:26 |
| 129 | Julien Bérard | AG2R La Mondiale | + 26:26 | + 26:26 |
| 130 | Daniel Sesma Sorbet | Euskaltel-Euskadi | + 26:26 | + 26:26 |
| 131 | Alex Rasmussen | Team HTC - Highroad | + 26:26 | + 26:26 |
| 132 | Bjorn Selander | Team RadioShack | + 26:26 | + 26:26 |
| 133 | Gert Dockx | Omega Pharma-Lotto | + 26:26 | + 26:26 |
| 134 | Simon Zahner | BMC Racing Team | + 26:26 | + 26:26 |
| 135 | Miguel Minguez | Euskaltel-Euskadi | + 26:26 | + 26:26 |
| 136 | Eros Capecchi | Liquigas-Cannondale | + 26:26 | + 26:26 |
| 137 | Mirko Selvaggi | Vacansoleil - DCM Pro Cycling Team | + 26:26 | + 26:26 |
| 138 | Javier Aramendia | Euskaltel-Euskadi | + 26:26 | + 26:26 |
| 139 | José Ochoa Carles | Androni Giocattoli - CIPI | + 26:26 | + 26:26 |
| 140 | Maxim Gourov | Pro Team Astana | + 26:26 | + 26:26 |
| 141 | Patrick Gretsch | Team HTC - Highroad | + 26:26 | + 26:26 |
| 142 | Olivier Kaisen | Omega Pharma-Lotto | + 26:26 | + 26:26 |
| 143 | Dennis van Winden | Rabobank | + 26:26 | + 26:26 |
| 144 | Alexsandr Dyachenko | Pro Team Astana | + 26:26 | + 26:26 |
| 145 | Gianluca Brambilla | Colnago - CSF Inox | + 26:26 | + 26:26 |
| 146 | Chad Beyer | BMC Racing Team | + 26:26 | + 26:26 |
| 147 | Kasper Klostergaard | Saxo Bank - SunGard | + 26:26 | + 26:26 |
| 148 | Leonardo Giordani | Farnese Vini - Neri Sottoli | + 26:26 | + 26:26 |
| 149 | José Serpa | Androni Giocattoli - CIPI | + 26:26 | + 26:26 |
| 150 | Alberto Ongarato | Vacansoleil - DCM Pro Cycling Team | + 26:26 | + 26:26 |
| 151 | Elia Favilli | Farnese Vini - Neri Sottoli | + 26:26 | + 26:26 |
| 152 | Fabio Taborre | Acqua e Sapone | + 26:26 | + 26:26 |
| 153 | Yaroslav Popovych | Team RadioShack | + 26:26 | + 26:26 |
| 154 | Ivan Rovny | Team RadioShack | + 26:26 | + 26:26 |
| 155 | Mauricio Ardila | Geox-TMC | + 26:26 | + 26:26 |
| 156 | Alexander Kristoff | BMC Racing Team | + 26:26 | + 26:26 |
| 157 | Ruggero Marzoli | Acqua e Sapone | + 26:38 | + 26:38 |
| 158 | Jos van Emden | Rabobank | + 26:49 | + 26:49 |
| 159 | Russell Downing | Sky ProCycling | + 27:26 | + 27:26 |