| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Damiano Cunego | Lampre | 05:37:04 | |
| 2 | Janez Brajkovic | Astana | + 24 | + 24 |
| 3 | Rigoberto Uran | Caisse d'Epargne | + 24 | + 24 |
| 4 | Giovanni Visconti | Quick Step | + 33 | + 33 |
| 5 | Karsten Kroon | Team CSC - Saxo Bank | + 33 | + 33 |
| 6 | Mauro Finetto | CSF Group - Navigare | + 33 | + 33 |
| 7 | Chris Horner | Astana | + 33 | + 33 |
| 8 | Stefano Garzelli | Acqua e Sapone - Caffe Mokambo | + 33 | + 33 |
| 9 | Morris Possoni | Team Columbia | + 33 | + 33 |
| 10 | Francesco Failli | Acqua e Sapone - Caffe Mokambo | + 33 | + 33 |
| 11 | Craig Lewis | Team Columbia | + 33 | + 33 |
| 12 | Christian Pfannberger | Barloworld | + 33 | + 33 |
| 13 | Alexandr Kolobnev | Team CSC - Saxo Bank | + 33 | + 33 |
| 14 | Alessandro Ballan | Lampre | + 33 | + 33 |
| 15 | Matteo Tosatto | Quick Step | + 33 | + 33 |
| 16 | Federico Canuti | CSF Group - Navigare | + 33 | + 33 |
| 17 | Andrea Noè | Liquigas | + 33 | + 33 |
| 18 | Matthew Lloyd | Silence - Lotto | + 33 | + 33 |
| 19 | Luca Mazzanti | Tinkoff Credit Systems | + 33 | + 33 |
| 20 | Rinaldo Nocentini | AG2R La Mondiale | + 33 | + 33 |
| 21 | Pieter Weening | Rabobank | + 33 | + 33 |
| 22 | Domenico Pozzovivo | CSF Group - Navigare | + 01:04 | + 01:04 |
| 23 | Roger Beuchat | Serramenti - Androni Giocattoli | + 01:34 | + 01:34 |
| 24 | Sylwester Szmyd | Lampre | + 01:34 | + 01:34 |
| 25 | Manuele Mori | Scott - American Beef | + 01:34 | + 01:34 |
| 26 | Cadel Evans | Silence - Lotto | + 01:34 | + 01:34 |
| 27 | Samuel Sánchez | Euskaltel-Euskadi | + 01:34 | + 01:34 |
| 28 | Joaquim Rodríguez | Caisse d'Epargne | + 01:34 | + 01:34 |
| 29 | Jurgen van Goolen | Team CSC - Saxo Bank | + 02:20 | + 02:20 |
| 30 | Serguei Klimov | Tinkoff Credit Systems | + 02:20 | + 02:20 |
| 31 | Maxime Monfort | Cofidis | + 02:20 | + 02:20 |
| 32 | Andrea Masciarelli | Acqua e Sapone - Caffe Mokambo | + 02:20 | + 02:20 |
| 33 | Mauricio Soler | Barloworld | + 02:20 | + 02:20 |
| 34 | Daniel Moreno | Caisse d'Epargne | + 02:20 | + 02:20 |
| 35 | Gilberto Simoni | Serramenti - Androni Giocattoli | + 02:20 | + 02:20 |
| 36 | Vladimir Miholjevic | Liquigas | + 02:20 | + 02:20 |
| 37 | Vincenzo Nibali | Liquigas | + 02:20 | + 02:20 |
| 38 | Kanstantsin Sivtsov | Team Columbia | + 02:20 | + 02:20 |
| 39 | Leonardo Bertagnolli | Liquigas | + 02:20 | + 02:20 |
| 40 | Massimo Codol | Acqua e Sapone - Caffe Mokambo | + 02:26 | + 02:26 |
| 41 | Niklas Axelsson | Serramenti - Androni Giocattoli | + 03:04 | + 03:04 |
| 42 | Ivan Velasco | Euskaltel-Euskadi | + 03:17 | + 03:17 |
| 43 | John Gadret | AG2R La Mondiale | + 03:17 | + 03:17 |
| 44 | Alberto Losada | Caisse d'Epargne | + 03:17 | + 03:17 |
| 45 | Marco Pinotti | Team Columbia | + 04:10 | + 04:10 |
| 46 | Francesco Gavazzi | Lampre | + 04:10 | + 04:10 |
| 47 | Martin Velits | Team Milram | + 05:52 | + 05:52 |
| 48 | Andriy Grivko | Team Milram | + 05:52 | + 05:52 |
| 49 | Walter Pedraza | Tinkoff Credit Systems | + 05:52 | + 05:52 |
| 50 | Alan Perez Lezaun | Euskaltel-Euskadi | + 05:52 | + 05:52 |
| 51 | Iñigo Landaluze | Euskaltel-Euskadi | + 05:52 | + 05:52 |
| 52 | Michael Barry | Team Columbia | + 05:52 | + 05:52 |
| 53 | Gorazd Stangelj | Liquigas | + 05:52 | + 05:52 |
| 54 | Gustav Larsson | Team CSC - Saxo Bank | + 05:52 | + 05:52 |
| 55 | Francesco Bellotti | Barloworld | + 05:52 | + 05:52 |
| 56 | Danilo Hondo | Serramenti - Androni Giocattoli | + 07:37 | + 07:37 |
| 57 | Michele Scarponi | Serramenti - Androni Giocattoli | + 07:37 | + 07:37 |
| 58 | Pablo Lastras | Caisse d'Epargne | + 07:37 | + 07:37 |
| 59 | Robert Gesink | Rabobank | + 09:27 | + 09:27 |
| 60 | Grischa Niermann | Rabobank | + 09:27 | + 09:27 |
| 61 | Ermanno Capelli | Scott - American Beef | + 09:27 | + 09:27 |
| 62 | René Mandri | AG2R La Mondiale | + 09:27 | + 09:27 |
| 63 | Wim van Huffel | Silence - Lotto | + 09:27 | + 09:27 |
| 64 | Kjell Carlström | Liquigas | + 09:27 | + 09:27 |
| 65 | Andrea Tonti | Quick Step | + 09:27 | + 09:27 |
| 66 | Sébastien Minard | Cofidis | + 09:27 | + 09:27 |
| 67 | Roberto Traficante | Serramenti - Androni Giocattoli | + 09:27 | + 09:27 |
| 68 | Gabriele Missaglia | Serramenti - Androni Giocattoli | + 09:27 | + 09:27 |
| 69 | Evgeni Petrov | Tinkoff Credit Systems | + 09:27 | + 09:27 |
| 70 | Anker Sørensen Chris | Team CSC - Saxo Bank | + 09:27 | + 09:27 |
| 71 | Volodymir Gustov | Team CSC - Saxo Bank | + 09:27 | + 09:27 |
| 72 | Valerio Agnoli | Liquigas | + 09:27 | + 09:27 |
| 73 | Pavel Brutt | Tinkoff Credit Systems | + 09:27 | + 09:27 |
| 74 | Steve Cummings | Barloworld | + 09:27 | + 09:27 |
| 75 | Jean-eudes Demaret | Cofidis | + 09:27 | + 09:27 |
| 76 | Imanol Erviti | Caisse d'Epargne | + 09:27 | + 09:27 |
| 77 | Michael Rogers | Team Columbia | + 09:27 | + 09:27 |
| 78 | Davide Vigano | Quick Step | + 09:27 | + 09:27 |
| 79 | Nicolas Hartmann | Cofidis | + 10:17 | + 10:17 |
| 80 | Francesco Masciarelli | Acqua e Sapone - Caffe Mokambo | + 10:27 | + 10:27 |
| 81 | Michael Schär | Astana | + 11:19 | + 11:19 |
| 82 | Matej Mugerli | Liquigas | + 11:19 | + 11:19 |
| 83 | Marlon Perez | Caisse d'Epargne | + 11:19 | + 11:19 |
| 84 | Javier Aramendia | Euskaltel-Euskadi | + 11:19 | + 11:19 |
| 85 | Olivier Bonnaire | Bouygues Telecom | + 11:19 | + 11:19 |
| 86 | Roman Kireyev | Astana | + 11:19 | + 11:19 |
| 87 | Benoit Joachim | Astana | + 11:19 | + 11:19 |
| 88 | John-lee Augustyn | Barloworld | + 11:19 | + 11:19 |
| 89 | Matthieu Ladagnous | Française des Jeux | + 11:19 | + 11:19 |
| 90 | Giuseppe Palumbo | Acqua e Sapone - Caffe Mokambo | + 11:19 | + 11:19 |
| 91 | Florent Brard | Cofidis | + 11:19 | + 11:19 |
| 92 | Lucas Euser | Garmin-Chipotle p/b H30 | + 11:19 | + 11:19 |
| 93 | Kevin Hulsmans | Quick Step | + 16:20 | + 16:20 |
| 94 | Tom Danielson | Garmin-Chipotle p/b H30 | + 16:20 | + 16:20 |