| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Alejandro Valverde | Caisse d'Epargne | 05:29:11 | |
| 2 | Alexandr Kolobnev | Team CSC - Saxo Bank | + 00 | |
| 3 | Davide Rebellin | Gerolsteiner | + 00 | |
| 4 | Paolo Bettini | Quick Step | + 00 | |
| 5 | Franco Pellizotti | Liquigas | + 00 | |
| 6 | Denis Menchov | Rabobank | + 00 | |
| 7 | Samuel Sánchez | Euskaltel-Euskadi | + 00 | |
| 8 | Stéphane Goubert | AG2R La Mondiale | + 00 | |
| 9 | Haimar Zubeldia | Euskaltel-Euskadi | + 02 | + 02 |
| 10 | David Moncoutié | Cofidis | + 02 | + 02 |
| 11 | Yaroslav Popovych | Silence - Lotto | + 12 | + 12 |
| 12 | Joaquim Rodríguez | Caisse d'Epargne | + 17 | + 17 |
| 13 | Thomas Voeckler | Bouygues Telecom | + 54 | + 54 |
| 14 | Sylvain Chavanel | Cofidis | + 01:03 | + 01:03 |
| 15 | David Arroyo | Caisse d'Epargne | + 01:03 | + 01:03 |
| 16 | David de La Fuente | Scott - American Beef | + 01:33 | + 01:33 |
| 17 | Marzio Bruseghin | Lampre | + 01:33 | + 01:33 |
| 18 | Andy Schleck | Team CSC - Saxo Bank | + 01:33 | + 01:33 |
| 19 | Vladimir Karpets | Caisse d'Epargne | + 01:36 | + 01:36 |
| 20 | Roman Kreuziger | Liquigas | + 01:36 | + 01:36 |
| 21 | Andrea Tonti | Quick Step | + 01:44 | + 01:44 |
| 22 | Michael Barry | Team Columbia | + 01:44 | + 01:44 |
| 23 | Eduard Vorganov | Xacobeo-Galicia | + 01:44 | + 01:44 |
| 24 | Gorka Verdugo | Euskaltel-Euskadi | + 01:48 | + 01:48 |
| 25 | Kanstantsin Sivtsov | Team Columbia | + 01:48 | + 01:48 |
| 26 | Alberto Contador | Astana | + 01:48 | + 01:48 |
| 27 | Vincenzo Nibali | Liquigas | + 03:31 | + 03:31 |
| 28 | Luis Arrieta José | AG2R La Mondiale | + 03:44 | + 03:44 |
| 29 | Andrea Noè | Liquigas | + 05:17 | + 05:17 |
| 30 | Manuel Garate Juan | Quick Step | + 08:35 | + 08:35 |
| 31 | Jurgen van de Walle | Quick Step | + 08:35 | + 08:35 |
| 32 | Yannick Talabardon | Crédit Agricole | + 09:23 | + 09:23 |
| 33 | Martin Velits | Team Milram | + 09:23 | + 09:23 |
| 34 | David García Dapena | Xacobeo-Galicia | + 09:34 | + 09:34 |
| 35 | Rubén Pérez Moreno | Euskaltel-Euskadi | + 09:34 | + 09:34 |
| 36 | Damiano Cunego | Lampre | + 09:34 | + 09:34 |
| 37 | Morris Possoni | Team Columbia | + 09:34 | + 09:34 |
| 38 | Vladimir Miholjevic | Liquigas | + 09:34 | + 09:34 |
| 39 | Gorazd Stangelj | Liquigas | + 09:34 | + 09:34 |
| 40 | Paolo Tiralongo | Lampre | + 09:34 | + 09:34 |
| 41 | Matteo Carrara | Quick Step | + 09:34 | + 09:34 |
| 42 | Pablo Lastras | Caisse d'Epargne | + 09:34 | + 09:34 |
| 43 | Benjamin Noval | Astana | + 13:12 | + 13:12 |
| 44 | Alberto Losada | Caisse d'Epargne | + 15:52 | + 15:52 |
| 45 | Alan Perez Lezaun | Euskaltel-Euskadi | + 15:52 | + 15:52 |
| 46 | Sergio Ghisalberti | Team Milram | + 15:52 | + 15:52 |