| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | León Sánchez Luis | Caisse d'Epargne | 05:47:13 | |
| 2 | Alexandre Vinokourov | Astana | + 00 | |
| 3 | Carlos Sastre | Cervelo TestTeam | + 00 | |
| 4 | Haimar Zubeldia | RadioShack | + 34 | + 34 |
| 5 | Joaquim Rodríguez | Team Katusha | + 37 | + 37 |
| 6 | Ryder Hesjedal | Garmin-Transitions | + 37 | + 37 |
| 7 | Robert Gesink | Rabobank | + 37 | + 37 |
| 8 | Nicolas Roche | AG2R La Mondiale | + 37 | + 37 |
| 9 | Samuel Sánchez | Euskaltel-Euskadi | + 37 | + 37 |
| 10 | Richie Porte | Team Saxo Bank | + 37 | + 37 |
| 11 | Greg van Avermaet | Omega Pharma-Lotto | + 02:14 | + 02:14 |
| 12 | Carlos Barredo | Quick Step | + 02:14 | + 02:14 |
| 13 | Anker Sørensen Chris | Team Saxo Bank | + 02:14 | + 02:14 |
| 14 | Javier Moreno | Andalucía - Cajasur | + 02:14 | + 02:14 |
| 15 | Eros Capecchi | Footon-Servetto | + 03:43 | + 03:43 |
| 16 | Christian Knees | Team Milram | + 03:43 | + 03:43 |
| 17 | Rubén Plaza | Caisse d'Epargne | + 03:43 | + 03:43 |
| 18 | Iván Gutiérrez | Caisse d'Epargne | + 03:43 | + 03:43 |
| 19 | Imanol Erviti | Caisse d'Epargne | + 03:43 | + 03:43 |
| 20 | André Steensen | Team Saxo Bank | + 03:43 | + 03:43 |
| 21 | Iñaki Isasi | Euskaltel-Euskadi | + 03:43 | + 03:43 |
| 22 | Christophe Le Mével | Française des Jeux | + 03:43 | + 03:43 |
| 23 | David García Dapena | Xacobeo-Galicia | + 03:43 | + 03:43 |
| 24 | Gorka Verdugo | Euskaltel-Euskadi | + 03:43 | + 03:43 |
| 25 | Damiano Cunego | Lampre-Farnese Vini | + 03:43 | + 03:43 |
| 26 | Morris Possoni | Sky Professional Cycling Team | + 03:43 | + 03:43 |
| 27 | Dries Devenyns | Quick Step | + 03:43 | + 03:43 |
| 28 | Giampaolo Caruso | Ceramica Flaminia | + 03:43 | + 03:43 |
| 29 | Daniel Moreno | Omega Pharma-Lotto | + 03:43 | + 03:43 |
| 30 | Laurent Didier | Team Saxo Bank | + 03:43 | + 03:43 |
| 31 | Luis Pasamontes | Caisse d'Epargne | + 03:43 | + 03:43 |
| 32 | Jakob Fuglsang | Team Saxo Bank | + 03:43 | + 03:43 |
| 33 | Robert Kiserlovski | Liquigas-Doimo | + 03:43 | + 03:43 |
| 34 | Ezequiel Mosquera | Xacobeo-Galicia | + 03:43 | + 03:43 |
| 35 | David Arroyo | Caisse d'Epargne | + 03:43 | + 03:43 |
| 36 | Gustavo Veloso | Xacobeo-Galicia | + 03:43 | + 03:43 |
| 37 | Igor Anton | Euskaltel-Euskadi | + 03:43 | + 03:43 |
| 38 | Levi Leipheimer | RadioShack | + 03:43 | + 03:43 |
| 39 | Volodymir Gustov | Cervelo TestTeam | + 03:43 | + 03:43 |
| 40 | Alberto Benitez Jose | Footon-Servetto | + 07:28 | + 07:28 |
| 41 | Marco Pinotti | Team HTC - Columbia | + 07:28 | + 07:28 |
| 42 | Nicki Sørensen | Team Saxo Bank | + 07:28 | + 07:28 |
| 43 | Philippe Gilbert | Omega Pharma-Lotto | + 07:28 | + 07:28 |
| 44 | Marcos Garcia | Xacobeo-Galicia | + 07:28 | + 07:28 |
| 45 | Jurgen van de Walle | Quick Step | + 07:28 | + 07:28 |
| 46 | Alexandr Kolobnev | Team Katusha | + 07:28 | + 07:28 |
| 47 | José Gomez | Andalucía - Cajasur | + 07:28 | + 07:28 |
| 48 | Antonio Flecha Juan | Sky Professional Cycling Team | + 07:28 | + 07:28 |
| 49 | David de La Fuente | Astana | + 07:28 | + 07:28 |
| 50 | Stefan Denifl | Cervelo TestTeam | + 07:28 | + 07:28 |
| 51 | David López | Caisse d'Epargne | + 07:28 | + 07:28 |
| 52 | Anthony Roux | Française des Jeux | + 09:01 | + 09:01 |
| 53 | Niki Terpstra | Team Milram | + 09:44 | + 09:44 |
| 54 | Manuel Garate Juan | Rabobank | + 09:44 | + 09:44 |
| 55 | Valerio Agnoli | Liquigas-Doimo | + 09:44 | + 09:44 |
| 56 | Steven Kruijswijk | Rabobank | + 09:44 | + 09:44 |
| 57 | Angel Vicioso | Andalucía - Cajasur | + 09:44 | + 09:44 |
| 58 | David Gutierrez G. | Footon-Servetto | + 09:44 | + 09:44 |
| 59 | Gorazd Stangelj | Astana | + 09:44 | + 09:44 |
| 60 | Martin Elmiger | AG2R La Mondiale | + 09:44 | + 09:44 |
| 61 | Luca Mazzanti | Team Katusha | + 09:44 | + 09:44 |
| 62 | Francesco Gavazzi | Lampre-Farnese Vini | + 09:44 | + 09:44 |
| 63 | Markel Irizar | RadioShack | + 09:44 | + 09:44 |
| 64 | Tejay van Garderen | Team HTC - Columbia | + 09:44 | + 09:44 |
| 65 | Jelle Vanendert | Omega Pharma-Lotto | + 09:44 | + 09:44 |
| 66 | Jan Bakelants | Omega Pharma-Lotto | + 09:44 | + 09:44 |
| 67 | Mickaël Delage | Omega Pharma-Lotto | + 09:44 | + 09:44 |
| 68 | Gonzalo Rabuñal | Xacobeo-Galicia | + 11:59 | + 11:59 |
| 69 | Luis Rubiera José | RadioShack | + 11:59 | + 11:59 |
| 70 | Rubén Pérez Moreno | Euskaltel-Euskadi | + 11:59 | + 11:59 |
| 71 | Alan Perez Lezaun | Euskaltel-Euskadi | + 11:59 | + 11:59 |
| 72 | Maxim Iglinskiy | Astana | + 11:59 | + 11:59 |
| 73 | Íñigo Cuesta | Cervelo TestTeam | + 11:59 | + 11:59 |
| 74 | Luis Arrieta José | AG2R La Mondiale | + 11:59 | + 11:59 |
| 75 | Delio Fernandez | Xacobeo-Galicia | + 11:59 | + 11:59 |
| 76 | Vincenzo Nibali | Liquigas-Doimo | + 11:59 | + 11:59 |
| 77 | Daniele Pietropolli | Lampre-Farnese Vini | + 11:59 | + 11:59 |
| 78 | Marco Marzano | Lampre-Farnese Vini | + 11:59 | + 11:59 |
| 79 | Thibaut Pinot | Française des Jeux | + 11:59 | + 11:59 |
| 80 | Arthur Vichot | Française des Jeux | + 11:59 | + 11:59 |
| 81 | John-lee Augustyn | Sky Professional Cycling Team | + 11:59 | + 11:59 |
| 82 | Francesco Bellotti | Liquigas-Doimo | + 11:59 | + 11:59 |
| 83 | Pedro Merino Criado | Footon-Servetto | + 11:59 | + 11:59 |
| 84 | Francesco Reda | Quick Step | + 11:59 | + 11:59 |
| 85 | Laurens ten Dam | Rabobank | + 11:59 | + 11:59 |
| 86 | Martin Montenegro Adaro Jorge | Andalucía - Cajasur | + 17:18 | + 17:18 |
| 87 | Julien Loubet | AG2R La Mondiale | + 17:18 | + 17:18 |
| 88 | Fabio Felline | Footon-Servetto | + 17:18 | + 17:18 |
| 89 | Josep Jufré | Astana | + 17:18 | + 17:18 |
| 90 | Gert Dockx | Team HTC - Columbia | + 17:18 | + 17:18 |
| 91 | Serafín Martinez | Xacobeo-Galicia | + 17:18 | + 17:18 |
| 92 | Andrey Kashechkin | Lampre-Farnese Vini | + 17:18 | + 17:18 |
| 93 | Steven Cozza | Garmin-Transitions | + 17:18 | + 17:18 |
| 94 | Manuel Ortega | Andalucía - Cajasur | + 17:18 | + 17:18 |