| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Alejandro Valverde | Caisse d'Epargne | 04:04:58 | |
| 2 | Egoi Martinez | Euskaltel-Euskadi | + 00 | |
| 3 | Jose Herrada | Contentpolis - Ampo | + 00 | |
| 4 | Mikel Astarloza | Euskaltel-Euskadi | + 00 | |
| 5 | Vincenzo Nibali | Liquigas | + 00 | |
| 6 | Damiano Cunego | Lampre-NGC | + 00 | |
| 7 | David Blanco | Palmeiras Resort - Prio | + 00 | |
| 8 | Gorka Verdugo | Euskaltel-Euskadi | + 00 | |
| 9 | Ezequiel Mosquera | Xacobeo-Galicia | + 00 | |
| 10 | Angel Vicioso | Andalucía - Cajasur | + 00 | |
| 11 | Fränk Schleck | Team Saxo Bank | + 00 | |
| 12 | Joaquim Rodríguez | Caisse d'Epargne | + 11 | + 11 |
| 13 | Aitor Hernández | Euskaltel-Euskadi | + 32 | + 32 |
| 14 | Jonathan Castroviejo | Orbea | + 39 | + 39 |
| 15 | Karsten Kroon | Team Saxo Bank | + 39 | + 39 |
| 16 | Julian Sanchez | Contentpolis - Ampo | + 39 | + 39 |
| 17 | Antonio Piedra | Andalucía - Cajasur | + 39 | + 39 |
| 18 | Ivan Velasco | Euskaltel-Euskadi | + 39 | + 39 |
| 19 | Mikel Nieve | Euskaltel-Euskadi | + 39 | + 39 |
| 20 | Marcos Garcia | Xacobeo-Galicia | + 41 | + 41 |
| 21 | Ricardo Serrano | Fuji-Servetto | + 41 | + 41 |
| 22 | David Arroyo | Caisse d'Epargne | + 41 | + 41 |
| 23 | Angel Vallejo Dominquez | Andorra - GrandValira | + 41 | + 41 |
| 24 | Dionisio Galparsoro | Contentpolis - Ampo | + 41 | + 41 |
| 25 | Gustav Larsson | Team Saxo Bank | + 47 | + 47 |
| 26 | Igor Astarloa | Amica Chips - Knauf | + 47 | + 47 |
| 27 | David de La Fuente | Fuji-Servetto | + 47 | + 47 |
| 28 | Jorge Azanza | Euskaltel-Euskadi | + 47 | + 47 |
| 29 | Branislau Samoilau | Quick Step | + 47 | + 47 |
| 30 | Antonio Redondo Jose | Andalucía - Cajasur | + 47 | + 47 |
| 31 | Iñigo Landaluze | Euskaltel-Euskadi | + 47 | + 47 |
| 32 | Javier Etxarri | Contentpolis - Ampo | + 01:04 | + 01:04 |
| 33 | Anker Sørensen Chris | Team Saxo Bank | + 01:04 | + 01:04 |
| 34 | Miguel Minguez | Orbea | + 01:04 | + 01:04 |
| 35 | Nicki Sørensen | Team Saxo Bank | + 01:04 | + 01:04 |
| 36 | Francisco Mourón Juan | Xacobeo-Galicia | + 01:04 | + 01:04 |
| 37 | Eduard Prades | Andorra - GrandValira | + 01:21 | + 01:21 |
| 38 | Amets Txurruka | Euskaltel-Euskadi | + 01:21 | + 01:21 |
| 39 | Eddy Ratti | Amica Chips - Knauf | + 01:23 | + 01:23 |
| 40 | Alejandro Marque | Palmeiras Resort - Prio | + 02:15 | + 02:15 |
| 41 | Samuel Caldeira | Palmeiras Resort - Prio | + 02:15 | + 02:15 |
| 42 | Alexander Ryabkin | Lokomotiv | + 02:18 | + 02:18 |
| 43 | Jurgen van Goolen | Team Saxo Bank | + 02:23 | + 02:23 |
| 44 | Edoardo Girardi | Amica Chips - Knauf | + 02:23 | + 02:23 |
| 45 | Iban Mayoz | Xacobeo-Galicia | + 02:23 | + 02:23 |
| 46 | Antonio Olmo | Boyaca es para Vivirla | + 02:23 | + 02:23 |
| 47 | Rafael Infantino | Amica Chips - Knauf | + 02:23 | + 02:23 |
| 48 | Gorka Verdugo | Euskaltel-Euskadi | + 02:23 | + 02:23 |
| 49 | Aitor Perez Arrieta | Contentpolis - Ampo | + 02:23 | + 02:23 |
| 50 | Diego Gallego | Burgos Monumental - Castilla y Leon | + 02:23 | + 02:23 |
| 51 | Raul Santamarta Valvuena | Burgos Monumental - Castilla y Leon | + 02:23 | + 02:23 |
| 52 | Avelino Antuna Coro Andres | Burgos Monumental - Castilla y Leon | + 02:23 | + 02:23 |
| 53 | Manuel Vazquez | Contentpolis - Ampo | + 02:23 | + 02:23 |
| 54 | David Belda | Boyaca es para Vivirla | + 02:23 | + 02:23 |
| 55 | Jaume Rovira | Andorra - GrandValira | + 02:40 | + 02:40 |
| 56 | Serafín Martinez | Xacobeo-Galicia | + 02:51 | + 02:51 |
| 57 | Dmitriy Ignatiev | Lokomotiv | + 04:18 | + 04:18 |
| 58 | Alejandro Paleo | Xacobeo-Galicia | + 04:18 | + 04:18 |
| 59 | Andrea Noè | Liquigas | + 04:18 | + 04:18 |
| 60 | Arkimedes Arguelyes | + 04:18 | + 04:18 | |
| 61 | Luis Silva | Palmeiras Resort - Prio | + 04:18 | + 04:18 |
| 62 | Henrique Casimiro | Palmeiras Resort - Prio | + 04:18 | + 04:18 |
| 63 | Nelson Vitorino | Palmeiras Resort - Prio | + 04:18 | + 04:18 |
| 64 | Eritz Ruiz | Orbea | + 04:18 | + 04:18 |
| 65 | Benat Urain Epelde | Orbea | + 04:18 | + 04:18 |
| 66 | Eduard Vorganov | Xacobeo-Galicia | + 05:53 | + 05:53 |
| 67 | Alessandro Vanotti | Liquigas | + 06:22 | + 06:22 |
| 68 | Ricardo Garcia | Orbea | + 06:22 | + 06:22 |
| 69 | Eneko Echeverz Carte | Orbea | + 06:22 | + 06:22 |
| 70 | Artem Ovechkin | Team Katusha | + 06:22 | + 06:22 |
| 71 | Josep Jufré | Fuji-Servetto | + 06:22 | + 06:22 |
| 72 | Raul Garcia de Mateos | Andorra - GrandValira | + 06:22 | + 06:22 |
| 73 | Manuel Ortega | Andalucía - Cajasur | + 06:22 | + 06:22 |
| 74 | Javier Estrada Juan | Andalucía - Cajasur | + 06:22 | + 06:22 |
| 75 | David López | Caisse d'Epargne | + 06:22 | + 06:22 |
| 76 | Brian Vandborg | Liquigas | + 06:22 | + 06:22 |
| 77 | Enrico Gasparotto | Lampre-NGC | + 06:22 | + 06:22 |
| 78 | Igor Anton | Euskaltel-Euskadi | + 10:32 | + 10:32 |
| 79 | Carlos Torrent | Andorra - GrandValira | + 10:32 | + 10:32 |
| 80 | Esteban Plaza Mira | Andalucía - Cajasur | + 10:32 | + 10:32 |
| 81 | Xabier Zandio | Caisse d'Epargne | + 10:32 | + 10:32 |
| 82 | Javier Mejias | Fuji-Servetto | + 10:32 | + 10:32 |
| 83 | Vicente Garcia de Mateos | Andorra - GrandValira | + 11:09 | + 11:09 |
| 84 | Valery Kaykov | Lokomotiv | + 11:09 | + 11:09 |
| 85 | José Casas Claudio | Contentpolis - Ampo | + 11:09 | + 11:09 |
| 86 | Aitor Olano Orbegozo | Orbea | + 11:09 | + 11:09 |
| 87 | Tiago Fiorilli | Boyaca es para Vivirla | + 11:09 | + 11:09 |
| 88 | Alexander Rybakov | Lokomotiv | + 11:09 | + 11:09 |
| 89 | Ramiro Rincón | Boyaca es para Vivirla | + 11:09 | + 11:09 |
| 90 | William Aranzazu Escobar | Boyaca es para Vivirla | + 11:09 | + 11:09 |
| 91 | Artur Ershov | + 11:09 | + 11:09 | |
| 92 | Vincenzo Garofalo | Amica Chips - Knauf | + 11:11 | + 11:11 |