| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Davide Rebellin | Gerolsteiner | 04:55:15 | |
| 2 | Danilo Di Luca | Liquigas | + 00 | |
| 3 | Giuseppe Di Grande | Miche | + 02 | + 02 |
| 4 | Santo Anzà | Selle Italia - Serramenti | + 10 | + 10 |
| 5 | Riccardo Riccò | Saunier Duval - Prodir | + 15 | + 15 |
| 6 | Simone Masciarelli | Acqua e Sapone - Caffe Mokambo | + 21 | + 21 |
| 7 | Ruggero Marzoli | Lampre-Fondital | + 21 | + 21 |
| 8 | Oliver Zaugg | Saunier Duval - Prodir | + 21 | + 21 |
| 9 | Luca Mazzanti | Ceramica Panaria - Navigare | + 30 | + 30 |
| 10 | Kanstantsin Sivtsov | Acqua e Sapone - Caffe Mokambo | + 33 | + 33 |
| 11 | John Gadret | AG2R Prévoyance | + 38 | + 38 |
| 12 | Alexandr Kolobnev | Rabobank | + 46 | + 46 |
| 13 | Alexandre Bazhenov | Naturino - Sapore di Mare | + 46 | + 46 |
| 14 | Alexander Arekeev | Acqua e Sapone - Caffe Mokambo | + 51 | + 51 |
| 15 | Przemyslaw Niemiec | Miche | + 01:01 | + 01:01 |
| 16 | Stéphane Goubert | AG2R Prévoyance | + 01:05 | + 01:05 |
| 17 | Fränk Schleck | Team CSC | + 01:18 | + 01:18 |
| 18 | Mauricio Soler | Acqua e Sapone - Caffe Mokambo | + 01:43 | + 01:43 |
| 19 | Theo Eltink | Rabobank | + 01:57 | + 01:57 |
| 20 | Mirko Celestino | Team Milram | + 02:08 | + 02:08 |
| 21 | Andrea Peron | Team CSC | + 02:31 | + 02:31 |
| 22 | Nicki Sørensen | Team CSC | + 02:31 | + 02:31 |
| 23 | Brian Vandborg | Team CSC | + 02:31 | + 02:31 |
| 24 | Manuele Spadi | Ceramica Flaminia | + 02:42 | + 02:42 |
| 25 | Leonardo Giordani | Naturino - Sapore di Mare | + 02:42 | + 02:42 |
| 26 | Julio Perez Cuapio | Ceramica Panaria - Navigare | + 02:42 | + 02:42 |
| 27 | Emanuele Sella | Ceramica Panaria - Navigare | + 02:47 | + 02:47 |
| 28 | Mikhaylo Khalilov | Team LPR | + 02:47 | + 02:47 |
| 29 | Andrea Moletta | Gerolsteiner | + 02:47 | + 02:47 |
| 30 | Andrea Tonti | Acqua e Sapone - Caffe Mokambo | + 02:51 | + 02:51 |
| 31 | Vincenzo Nibali | Liquigas | + 02:54 | + 02:54 |
| 32 | Frank Vandenbroucke | Unibet.com | + 03:00 | + 03:00 |
| 33 | Andrea Noè | Liquigas | + 03:00 | + 03:00 |
| 34 | Sylwester Szmyd | Lampre-Fondital | + 03:00 | + 03:00 |
| 35 | Marco Osella | Team 3C Casalinghi Jet | + 03:00 | + 03:00 |
| 36 | Guido Trentin | Saunier Duval - Prodir | + 03:00 | + 03:00 |
| 37 | Marlon Perez | Team Tenax Salmilano | + 03:16 | + 03:16 |
| 38 | Matteo Tosatto | Quick Step - Innergetic | + 03:16 | + 03:16 |
| 39 | Rigoberto Uran | Team Tenax Salmilano | + 03:28 | + 03:28 |
| 40 | Pieter Weening | Rabobank | + 03:28 | + 03:28 |
| 41 | Valerio Agnoli | Naturino - Sapore di Mare | + 03:41 | + 03:41 |
| 42 | Jamie Burrow | OTC Doors - Lauretana | + 03:41 | + 03:41 |
| 43 | Matteo Carrara | Lampre-Fondital | + 04:21 | + 04:21 |
| 44 | Paolo Bailetti | Team 3C Casalinghi Jet | + 04:21 | + 04:21 |
| 45 | Fabian Wegmann | Gerolsteiner | + 04:34 | + 04:34 |
| 46 | Niklas Axelsson | Selle Italia - Serramenti | + 05:56 | + 05:56 |
| 47 | Marco Pinotti | Saunier Duval - Prodir | + 06:42 | + 06:42 |
| 48 | Luca Solari | Team 3C Casalinghi Jet | + 06:42 | + 06:42 |
| 49 | Pedro Arreitunandia | Barloworld | + 07:13 | + 07:13 |