| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Robert Gesink | Rabobank | 04:49:32 | |
| 2 | Jakob Fuglsang | Team Saxo Bank | + 00 | |
| 3 | Thomas Löfkvist | Team Columbia - HTC | + 00 | |
| 4 | Cadel Evans | Silence - Lotto | + 20 | + 20 |
| 5 | Alexandre Vinokourov | Astana | + 30 | + 30 |
| 6 | Fredrik Kessiakoff | Fuji-Servetto | + 32 | + 32 |
| 7 | Alexandr Kolobnev | Team Saxo Bank | + 40 | + 40 |
| 8 | Anker Sørensen Chris | Team Saxo Bank | + 43 | + 43 |
| 9 | Alberto Fernandez | Fuji-Servetto | + 43 | + 43 |
| 10 | Paolo Tiralongo | Lampre-NGC | + 50 | + 50 |
| 11 | Mauricio Soler | Barloworld - Bianchi | + 53 | + 53 |
| 12 | Domenico Pozzovivo | CSF Group - Navigare | + 01:00 | + 01:00 |
| 13 | Bartosz Huzarski | ISD - Neri | + 01:00 | + 01:00 |
| 14 | Damiano Cunego | Lampre-NGC | + 01:03 | + 01:03 |
| 15 | Leonardo Bertagnolli | Serramenti - Androni Giocattoli | + 01:15 | + 01:15 |
| 16 | Ivan Basso | Liquigas | + 01:20 | + 01:20 |
| 17 | Maxime Monfort | Team Columbia - HTC | + 01:20 | + 01:20 |
| 18 | Chris Horner | Astana | + 01:20 | + 01:20 |
| 19 | Davide Malacarne | Quick Step | + 01:45 | + 01:45 |
| 20 | Emanuele Sella | Carmiooro - A-Style | + 02:01 | + 02:01 |
| 21 | Matthew Lloyd | Silence - Lotto | + 02:01 | + 02:01 |
| 22 | Sylwester Szmyd | Liquigas | + 02:07 | + 02:07 |
| 23 | Riccardo Chiarini | LPR Brakes - Farnese Vini | + 02:15 | + 02:15 |
| 24 | Ruslan Pidgornyy | ISD - Neri | + 02:20 | + 02:20 |
| 25 | Tadej Valjavec | AG2R La Mondiale | + 02:20 | + 02:20 |
| 26 | Angel Rubiano Miguel | Centri della Calzatura | + 02:20 | + 02:20 |
| 27 | Morris Possoni | Team Columbia - HTC | + 02:20 | + 02:20 |
| 28 | Enrico Zen | CSF Group - Navigare | + 02:20 | + 02:20 |
| 29 | Yauhen Sobal | Centri della Calzatura | + 02:20 | + 02:20 |
| 30 | Paul Martens | Rabobank | + 02:27 | + 02:27 |
| 31 | Gustav Larsson | Team Saxo Bank | + 02:27 | + 02:27 |
| 32 | Alexandre Botcharov | Team Katusha | + 02:27 | + 02:27 |
| 33 | Andrea Masciarelli | Acqua e Sapone - Caffe Mokambo | + 02:36 | + 02:36 |
| 34 | Leonardo Giordani | Ceramica Flaminia-Bossini Docce | + 02:36 | + 02:36 |
| 35 | Carlos Barredo | Quick Step | + 02:51 | + 02:51 |
| 36 | Luca Mazzanti | Team Katusha | + 02:57 | + 02:57 |
| 37 | Cristiano Salerno | LPR Brakes - Farnese Vini | + 03:02 | + 03:02 |
| 38 | Bauke Mollema | Rabobank | + 03:10 | + 03:10 |
| 39 | Luca Zanasca | Centri della Calzatura | + 03:16 | + 03:16 |
| 40 | Gorazd Stangelj | Liquigas | + 03:16 | + 03:16 |
| 41 | Craig Lewis | Team Columbia - HTC | + 03:25 | + 03:25 |
| 42 | Vladimir Karpets | Team Katusha | + 03:30 | + 03:30 |
| 43 | Francesco Failli | Acqua e Sapone - Caffe Mokambo | + 03:41 | + 03:41 |
| 44 | Francesco Bellotti | Barloworld - Bianchi | + 03:41 | + 03:41 |
| 45 | Francesco Masciarelli | Acqua e Sapone - Caffe Mokambo | + 03:50 | + 03:50 |
| 46 | Oliver Zaugg | Liquigas | + 03:57 | + 03:57 |
| 47 | Evgeni Petrov | Team Katusha | + 04:15 | + 04:15 |
| 48 | Vladimir Miholjevic | Liquigas | + 05:10 | + 05:10 |
| 49 | Alexsandr Dyachenko | Astana | + 05:10 | + 05:10 |
| 50 | Nicolas Roche | AG2R La Mondiale | + 05:43 | + 05:43 |
| 51 | Santo Anzà | ISD - Neri | + 07:30 | + 07:30 |
| 52 | Massimo Codol | Acqua e Sapone - Caffe Mokambo | + 08:30 | + 08:30 |
| 53 | Marco Marzano | Lampre-NGC | + 08:30 | + 08:30 |
| 54 | Hubert Schwab | Quick Step | + 08:30 | + 08:30 |
| 55 | Diego Caccia | Barloworld - Bianchi | + 08:30 | + 08:30 |
| 56 | Luca Solari | Serramenti - Androni Giocattoli | + 08:30 | + 08:30 |
| 57 | Paolo Longo Borghini | Barloworld - Bianchi | + 08:30 | + 08:30 |
| 58 | Paolo Bailetti | Fuji-Servetto | + 08:30 | + 08:30 |
| 59 | Dries Devenyns | Quick Step | + 08:30 | + 08:30 |
| 60 | Fabio Taborre | Serramenti - Androni Giocattoli | + 08:30 | + 08:30 |
| 61 | Alessandro Bisolti | CSF Group - Navigare | + 08:30 | + 08:30 |
| 62 | Manuele Mori | Lampre-NGC | + 08:30 | + 08:30 |