| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Fränk Schleck | Team CSC | 05:22:17 | |
| 2 | Davide Rebellin | Gerolsteiner | + 01 | + 01 |
| 3 | Chris Horner | Predictor - Lotto | + 05 | + 05 |
| 4 | Riccardo Riccò | Saunier Duval - Prodir | + 10 | + 10 |
| 5 | Damiano Cunego | Lampre-Fondital | + 18 | + 18 |
| 6 | Cadel Evans | Predictor - Lotto | + 25 | + 25 |
| 7 | Sylwester Szmyd | Lampre-Fondital | + 26 | + 26 |
| 8 | Luca Mazzanti | Ceramica Panaria - Navigare | + 48 | + 48 |
| 9 | Alexandr Kolobnev | Team CSC | + 56 | + 56 |
| 10 | Robert Gesink | Rabobank | + 01:00 | + 01:00 |
| 11 | Giairo Ermeti | Tenax - Menikini | + 01:19 | + 01:19 |
| 12 | Simone Masciarelli | Acqua e Sapone | + 01:55 | + 01:55 |
| 13 | Danilo Di Luca | Liquigas | + 02:01 | + 02:01 |
| 14 | Giovanni Visconti | Quick Step - Innergetic | + 02:01 | + 02:01 |
| 15 | Paolo Bailetti | Team LPR | + 02:02 | + 02:02 |
| 16 | Carlos Sastre | Team CSC | + 02:02 | + 02:02 |
| 17 | Dario Cioni | Predictor - Lotto | + 02:03 | + 02:03 |
| 18 | Leonardo Moser | Serramenti - Selle Italia | + 02:05 | + 02:05 |
| 19 | Hubert Schwab | Quick Step - Innergetic | + 02:05 | + 02:05 |
| 20 | Alexander Arekeev | Acqua e Sapone | + 02:08 | + 02:08 |
| 21 | Daniele Nardello | Team LPR | + 02:21 | + 02:21 |
| 22 | Hubert Krys | Ceramica Flaminia | + 02:47 | + 02:47 |
| 23 | Dario Cataldo | Liquigas | + 02:47 | + 02:47 |
| 24 | Niklas Axelsson | Serramenti - Selle Italia | + 02:47 | + 02:47 |
| 25 | Gilberto Simoni | Saunier Duval - Prodir | + 02:47 | + 02:47 |
| 26 | Pieter Weening | Rabobank | + 02:47 | + 02:47 |
| 27 | Alessandro Bertuola | Tenax - Menikini | + 02:47 | + 02:47 |
| 28 | Andrey Mizurov | Astana | + 02:47 | + 02:47 |
| 29 | Philip Deignan | AG2R Prévoyance | + 02:47 | + 02:47 |
| 30 | Evgeni Petrov | Tinkoff Credit Systems | + 02:55 | + 02:55 |
| 31 | Koos Moerenhout | Rabobank | + 03:01 | + 03:01 |
| 32 | Santo Anzà | Serramenti - Selle Italia | + 03:26 | + 03:26 |
| 33 | Emanuele Sella | Ceramica Panaria - Navigare | + 03:39 | + 03:39 |
| 34 | Luca Solari | Team LPR | + 03:39 | + 03:39 |
| 35 | Andrea Tonti | Quick Step - Innergetic | + 04:02 | + 04:02 |
| 36 | Marco Marzano | Lampre-Fondital | + 04:03 | + 04:03 |
| 37 | Matthew Lloyd | Predictor - Lotto | + 04:49 | + 04:49 |
| 38 | Cristiano Salerno | Tenax - Menikini | + 06:56 | + 06:56 |
| 39 | Blaise Sonnery | AG2R Prévoyance | + 06:58 | + 06:58 |
| 40 | Andrea Masciarelli | Acqua e Sapone | + 06:59 | + 06:59 |
| 41 | Domenico Pozzovivo | Ceramica Panaria - Navigare | + 07:07 | + 07:07 |
| 42 | Pedro Arreitunandia | Barloworld | + 07:09 | + 07:09 |