| # | Rider | Team | Time | Gap |
|---|---|---|---|---|
| 1 | Carlos Betancur | Acqua e Sapone | 05:05:05 | |
| 2 | Bauke Mollema | Rabobank | + 24 | + 24 |
| 3 | Rigoberto Uran | Sky ProCycling | + 24 | + 24 |
| 4 | Davide Rebellin | Miche - Guerciotti | + 24 | + 24 |
| 5 | Przemyslaw Niemiec | Lampre-ISD | + 24 | + 24 |
| 6 | Joaquim Rodríguez | Katusha Team | + 28 | + 28 |
| 7 | Riccardo Chiarini | Androni Giocattoli - CIPI | + 31 | + 31 |
| 8 | Domenico Pozzovivo | Colnago - CSF Inox | + 31 | + 31 |
| 9 | Oliver Zaugg | Leopard-Trek | + 33 | + 33 |
| 10 | Vincenzo Nibali | Liquigas-Cannondale | + 39 | + 39 |
| 11 | Christophe Le Mével | Garmin-Cervélo | + 40 | + 40 |
| 12 | Fabio Duarte | Geox-TMC | + 40 | + 40 |
| 13 | Thomas Löfkvist | Sky ProCycling | + 42 | + 42 |
| 14 | Daniel Moreno | Katusha Team | + 47 | + 47 |
| 15 | David de La Fuente | Geox-TMC | + 53 | + 53 |
| 16 | Fabio Taborre | Acqua e Sapone | + 54 | + 54 |
| 17 | Francesco Masciarelli | Astana | + 01:02 | + 01:02 |
| 18 | Andrey Zeits | Astana | + 01:15 | + 01:15 |
| 19 | Angel Rubiano Miguel | D'Angelo & Antenucci - Nippo | + 01:18 | + 01:18 |
| 20 | Stefan Schumacher | Miche - Guerciotti | + 01:19 | + 01:19 |
| 21 | Daniel Martin | Garmin-Cervélo | + 01:19 | + 01:19 |
| 22 | Fabio Felline | Geox-TMC | + 01:19 | + 01:19 |
| 23 | Federico Canuti | Colnago - CSF Inox | + 01:35 | + 01:35 |
| 24 | Ivan Basso | Liquigas-Cannondale | + 01:35 | + 01:35 |
| 25 | Emanuele Sella | Androni Giocattoli - CIPI | + 01:35 | + 01:35 |
| 26 | Robert Kiserlovski | Astana | + 01:35 | + 01:35 |
| 27 | Giovanni Visconti | Farnese Vini - Neri Sottoli | + 01:35 | + 01:35 |
| 28 | Gianluca Brambilla | Colnago - CSF Inox | + 02:17 | + 02:17 |
| 29 | Michael Rogers | Sky ProCycling | + 02:17 | + 02:17 |
| 30 | Jakob Fuglsang | Leopard-Trek | + 02:59 | + 02:59 |
| 31 | Alexsandr Dyachenko | Astana | + 03:18 | + 03:18 |
| 32 | Giampaolo Caruso | Katusha Team | + 03:18 | + 03:18 |
| 33 | Tom-jelte Slagter | Rabobank | + 04:49 | + 04:49 |
| 34 | Mathieu Perget | AG2R La Mondiale | + 04:49 | + 04:49 |
| 35 | Carlos Barredo | Rabobank | + 04:49 | + 04:49 |
| 36 | Cristiano Salerno | Liquigas-Cannondale | + 04:49 | + 04:49 |
| 37 | David Gutierrez G. | Geox-TMC | + 04:49 | + 04:49 |
| 38 | Damiano Caruso | Liquigas-Cannondale | + 04:49 | + 04:49 |
| 39 | Peter Stetina | Garmin-Cervélo | + 04:49 | + 04:49 |
| 40 | Rafal Majka | Saxo Bank - SunGard | + 04:49 | + 04:49 |
| 41 | Sylwester Szmyd | Liquigas-Cannondale | + 04:49 | + 04:49 |
| 42 | Evgeni Petrov | Astana | + 04:49 | + 04:49 |
| 43 | Laurens ten Dam | Rabobank | + 04:49 | + 04:49 |
| 44 | Steven Kruijswijk | Rabobank | + 04:49 | + 04:49 |
| 45 | Pieter Weening | Rabobank | + 04:49 | + 04:49 |
| 46 | David Le Lay | AG2R La Mondiale | + 05:36 | + 05:36 |
| 47 | Arthur Vichot | FDJ | + 05:36 | + 05:36 |
| 48 | Laurent Didier | Saxo Bank - SunGard | + 06:08 | + 06:08 |
| 49 | Silva Pires Bruno | Leopard-Trek | + 09:22 | + 09:22 |
| 50 | Rémi Pauriol | FDJ | + 09:22 | + 09:22 |
| 51 | Maxime Bouet | AG2R La Mondiale | + 09:22 | + 09:22 |
| 52 | Damiano Margutti | De Rosa - Ceramica Flaminia | + 09:22 | + 09:22 |
| 53 | Grischa Niermann | Rabobank | + 09:22 | + 09:22 |
| 54 | Thibaut Pinot | FDJ | + 09:22 | + 09:22 |